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Thromb Haemost 1992; 67(04): 478-483
DOI: 10.1055/s-0038-1648474
Original Articles
Schattauer GmbH Stuttgart

Transforming Growth Factor β (TGF-β)Inhibits Expression of Fibrinogen and Factor VII in a Hepatoma Cell Line

Jane H Hassan
1   The Department of Hematology and Oncology, Istituto Superiore di Sanita, Rome, Italy
2   European Molecular Biology Laboratory, Heidelberg, FRG
,
Cristiana Chelucci
1   The Department of Hematology and Oncology, Istituto Superiore di Sanita, Rome, Italy
,
Cesare Peschle
1   The Department of Hematology and Oncology, Istituto Superiore di Sanita, Rome, Italy
,
Vincenzo Sorrentino
2   European Molecular Biology Laboratory, Heidelberg, FRG
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Publikationsverlauf

Received 07. April 1991

Accepted after revision 09. Oktober 1991

Publikationsdatum:
03. Juli 2018 (online)

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Summary

We have investigated the effect(s) of transforming growth factor (TGF)-β1 and interleukin (IL)-6 on the expression of fibrinogen and blood coagulation factors VII, IX, X mRNAs in a hepatoma cell line (Hep 3B). The results indicate that TGF-β induces a decrease of the basal level of fibrinogen and factor VII mRNAs, but does not affect factor X expression. Furthermore, TGF-β efficiently antagonizes the IL-6 induction of fibrinogen mRNA at late (12-48 h) but not early (6 h) times: this effect is apparently mediated by posttranscriptional mechanism(s). These findings, together with previously reported data on the inhibitory effect of TGF-β on acute phase genes (e.g., ApoAl and albumin), suggest a role for TGF-β in the regulation of liver genes expression. The early stimulatory and late inhibitory effect exerted by IL-6 and TGF-β respectively on fibrinogen mRNA level may play a role in the regulatory mechanism(s) of clot formation in a variety of conditions.

 

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