Thromb Haemost 1977; 37(02): 283-290
DOI: 10.1055/s-0038-1649229
Original Article
Schattauer GmbH

Kinetic Parameters of Platelet Aggregation as an Expression of Platelet Responsiveness

Ennio C Rossi
1   Hematology Section, Department of Medicine, Northwestern University School of Medicine, Chicago, Illinois, USA
,
Gerlinde Louis
1   Hematology Section, Department of Medicine, Northwestern University School of Medicine, Chicago, Illinois, USA
› Author Affiliations
Further Information

Publication History

Received 30 September 1976

Accepted 20 December 1976

Publication Date:
03 July 2018 (online)

Summary

The kinetics of platelet aggregation induced by collagen and by ADP were studied. The maximum aggregation (ΔLTmax) and the ADP and collagen concentrations required to produce half-maximum aggregation (Kd) were determined using platelets obtained from normal individuals, individuals who ingested aspirin and individuals whose platelet-rich plasma (PRP) demonstrated spontaneous aggregation. The Kd and ΔLTmaxfor ADP-induced platelet aggregation were variable and markedly affected by the citrate concentration. Conversely, kinetic parameters of collagen-induced aggregation were more reproducible and less affected by citrate. The Kd for collagen in platelet aggregation was increased following aspirin ingestion and decreased in samples of PRP that demonstrated spontaneous aggregation. These results suggest that kinetic parameters of platelet aggregation may be useful to express the responsiveness of platelets.

 
  • References

  • 1 Born G. V. R. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature 1962; 194: 927
  • 2 Born G. V. R, Cross M. J. The aggregation of blood platelets. Journal of Physiology 1963; 168: 178
  • 3 Born G. V. R, Cross M. J. Effects of inorganic ions and of plasma proteins on the aggregation of blood platelets by adenosine diphosphate. Journal of Physiology 1964; 170: 397
  • 4 Born G. V. R, Hume H. Effects of the numbers of sizes of platelet aggregates on the optical density of plasma. Nature 1967; 215: 1027
  • 5 Day H. J, Hardisty R. M. Report of the task force on platelet standards. Thrombosis et Diathesis Haemorrhagica, Supplement 1974; 59: 307
  • 6 Frojmovic M. M. Quantitative parameterization of the light transmission properties of citrated platelet-rich plasma as a function of platelet and adenosine diphosphate concentrations and temperature. Journal of Laboratory and Clinical Medicine 1973; 82: 137
  • 7 Goldstein A, Aronow L, Kalman S. M. Consequences of drugreceptor interactions: Analysis of the graded dose-response relationship. In Principles of Drug Action: The Basis of Pharmacology (Second Edition). John Wiley and Sons; New York: 1974. p. 82
  • 8 Green D, Dunne B, Schmid F. R, Rossi E. C, Louis G. A study of the variable response of human platelets to collagen: relation to aspirin-induced inhibition of aggregation. American Journal of Clinical Pathology 1973; 60: 920
  • 9 Holmsen H. Biochemistry of the platelet release reaction. In Biochemistry and Pharmacology of Platelets (Ciba Foundation Symposium 35). Excerpta Medica Foundation; Amsterdam: 1975. p. 175
  • 10 Mürer E. H. Thrombin induced release of calcium from blood platelets. Science 1969; 166: 623
  • 11 Mürer E. H, Holme R. A study of the release of calcium from human blood platelets and its inhibition of metabolic inhibitors, N-ethylmaleimide and aspirin. Biochemica et Biophysica Acta 1970; 222: 197
  • 12 O’Brien J. R. Effects of salicylates on human platelets. Lancet 1968; 1: 779
  • 13 Rossi E. C, Levin N. W. Inhibition of ADP-induced aggregation by furosemide. Journal of Laboratory and Clinical Medicine 1973; a 81: 140
  • 14 Rossi E. C, Levin N. W. Inhibition of primary ADP-induced platelet aggregation in normal subjects after administration of nitrofurantoin (Furadantin). Journal of Clinical Investigation 1973; b 52: 2457
  • 15 Rossi E. C, Louis G. A time-dependent increase in the responsiveness of platelet-rich plasma to epinephrine. Journal of Laboratory and Clinical Medicine 1975; 85: 300
  • 16 Skoza L, Zucker M. B, Jerushalmy Z, Grant R. Kinetic studies of platelet aggregation induced by adenosine diphosphate and its inhibition by chelating agents, guanidino compounds, and adenosine. Thrombosis et Diathesis Haemorrhagica 1967; 18: 713
  • 17 Spaet T, Zucker M. B. Mechanism of platelet plug formation and role of adenosine diphosphate. American Journal of Physiology 1964; 206: 1267
  • 18 Weiss H. J, Albert L. M. Impaired platelet/connective tissue reaction in man after aspirin ingestion. Lancet 1967; 2: 495
  • 19 Woessner J. F Jr.. The determination of hydroxyproline in tissue and protein samples containing small proportions of imino acid. Archives of Biochemistry and Biophysics 1961; 93: 440