Subscribe to RSS
DOI: 10.1055/s-0038-1649255
A Method for the Measurement of Fibrinolytic Activity Based on One Dimensional Diffusion Using Small Glass Tubes
II. With Special Reference to the Differences between the Use of Plasminogen-Rich Fibrinogen and that of Plasminogen-Free Fibrinogen as the SubstratesPublication History
Received 24 April 1976
Accepted 28 February 1977
Publication Date:
03 July 2018 (online)

Summary
The optimal conditions for the measurement of the fibrinolytic factors of plasma were examined using human and bovine plasminogen-rich fibrinogen or plasminogen-free fibrinogen as the substrates using the one dimensional diffusion method.
The results were as follows:
1. There was no essential difference found between using human or bovine fibrinogen.
2. The levels of proactivator-plasminogen and plasminogen could be measured while using either plasminogen-rich or plasminogen-free fibrinogen. But, in using the latter, the proactivator-plasminogen level could not be measured, if a final concentration of more than 2,000 Christensen units of streptokinase were employed.
3. When using plasminogen-rich fibrinogen, anti-plasmin(s) and anti-activator(s) could be measured while using urokinase and plasmin, but not while using streptokinase. However, further study should be given to the measurement of the inhibitors, when using plasminogen-free fibrinogen.
-
References
- 1 Bachmann F. Development of Antibodies against Perorally and Rectally Administrated Streptokinase in Man. Journal of Laboratory and Clinical Medicine 1968; 72: 228
- 2 Brogden R. N, Speigt T. M, Avery G. S. A Review of its Clinical Pharmacology, Mechanism of Action and Therapeutic Uses. Drugs 1975; 5: 357
- 3 Deutsch D. G, Mertz E. T. Plasminogen: Purification from Human Plasma by Affinity Chromatography. Science 1970; 170: 1095
- 4 Downie G. R, Cliffton E. E. A Method for Assay of Serum Proteolytic Activity. Proceedings of the Society for Experimental Biology and Medicine 1949; 85: 142
- 5 James D. C. O. Anti Streptokinase Level in Various Hospital Patient Groups. Postgraduate Medical Journal (August Suppl.); 1973: 26
- 6 Kοκ P, Astrup T. Differentiation between Plasminogen Activators by Means of Epsilon-Aminocaproic Acid. Thrombosis et Diathesis Haemorrhagica 1972; 27: 77
- 7 Kwalski M, Kopek M, Niewaroski S. An Evaluation of Fibrinolysis. Journal of Clinical Pathology 1959; 12: 215
- 8 Maki M, Saito H, Yoneya T. Method of Antiplasmin Assay in Human Plasma. Tohoku Journal of Experimental Medicine 1964; 83: 168
- 9 Milston H. A Factor in Normal Human Blood which Participates in Streptococcal Fibrinolysis. Journal of Immunology 1941; 42: 109
- 10 Ouchterlony O. Diffusion-in-Gel Method for Immunological Analysis. In: Kallós, P. (ed.), Progress in Allergy. Vol. 5.S. Karger AG; Basel & New York: 1958: 16
- 11 Yasukouchi T, Watanabe T. A New Method Based on One Dimensional Diffusion Using Small Glass Tubes for the Measurement of Fibrinolytic Activity I. Fibrinolytic Activity of Plasmin and “Euglobulin + Streptokinase”. Thrombosis et Diathesis Haemorrhagica 1972; 28: 329