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Thromb Haemost 1977; 37(03): 456-463
DOI: 10.1055/s-0038-1649255
Original Article
Schattauer GmbH

A Method for the Measurement of Fibrinolytic Activity Based on One Dimensional Diffusion Using Small Glass Tubes

II. With Special Reference to the Differences between the Use of Plasminogen-Rich Fibrinogen and that of Plasminogen-Free Fibrinogen as the Substrates
Taro Yasukouchi
1   Second Department of Internal Medicine, Hokkaido University School of Medicine, West 5, North 14, Kita-ku, Sapporo 060, Japan
,
Masaru Fujine
1   Second Department of Internal Medicine, Hokkaido University School of Medicine, West 5, North 14, Kita-ku, Sapporo 060, Japan
,
Sukehiro Kohn
1   Second Department of Internal Medicine, Hokkaido University School of Medicine, West 5, North 14, Kita-ku, Sapporo 060, Japan
,
Shoki Sakurama
1   Second Department of Internal Medicine, Hokkaido University School of Medicine, West 5, North 14, Kita-ku, Sapporo 060, Japan
,
Tokiyo Morioka
1   Second Department of Internal Medicine, Hokkaido University School of Medicine, West 5, North 14, Kita-ku, Sapporo 060, Japan
,
Akio Mikami
1   Second Department of Internal Medicine, Hokkaido University School of Medicine, West 5, North 14, Kita-ku, Sapporo 060, Japan
,
Morimasa Iju
1   Second Department of Internal Medicine, Hokkaido University School of Medicine, West 5, North 14, Kita-ku, Sapporo 060, Japan
,
Shoichi Nakagawa
1   Second Department of Internal Medicine, Hokkaido University School of Medicine, West 5, North 14, Kita-ku, Sapporo 060, Japan
› Author Affiliations
Further Information

Publication History

Received 24 April 1976

Accepted 28 February 1977

Publication Date:
03 July 2018 (online)

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Summary

The optimal conditions for the measurement of the fibrinolytic factors of plasma were examined using human and bovine plasminogen-rich fibrinogen or plasminogen-free fibrinogen as the substrates using the one dimensional diffusion method.

The results were as follows:

1. There was no essential difference found between using human or bovine fibrinogen.

2. The levels of proactivator-plasminogen and plasminogen could be measured while using either plasminogen-rich or plasminogen-free fibrinogen. But, in using the latter, the proactivator-plasminogen level could not be measured, if a final concentration of more than 2,000 Christensen units of streptokinase were employed.

3. When using plasminogen-rich fibrinogen, anti-plasmin(s) and anti-activator(s) could be measured while using urokinase and plasmin, but not while using streptokinase. However, further study should be given to the measurement of the inhibitors, when using plasminogen-free fibrinogen.

 

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