Download PDF
Thromb Haemost 1993; 70(06): 0963-0969
DOI: 10.1055/s-0038-1649708
Original Article
Coagulation
Schattauer GmbH Stuttgart

Expression and Localization of Tissue Factor-Based Procoagulant Activity (PCA) in Pigeon Monocyte-Derived Macrophages

Madhu Gupta
1   The Department of Pathology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina, USA
,
George J Doellgas
2   The Department of Biochemistry, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina, USA
3   Elcatech Inc., Winston-Salem, North Carolina, USA
,
Therese Cheng
3   Elcatech Inc., Winston-Salem, North Carolina, USA
,
Jon C Lewis
1   The Department of Pathology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina, USA
› Author Affiliations
Further Information

Publication History

Received 09 March 1993

Accepted after revision 30 July 1993

Publication Date:
06 July 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

Monocyte-derived macrophages, focal to initiation and progression of atherosclerosis, have been implicated in thrombotic complication of this disease. In the present study we demonstrated tissue factor based procoagulant activity in cultured macrophages from the White Carneau pigeon following endotoxin (1-2 μg/ml) stimulation. This macrophage procoagulant activity paralleled activity obtained with pigeon brain homogenate. We used Enzyme-Linked Coagulation Assay (ELCA), an ultrasensitive microtiter plate assay, to measure procoagulant activity in these cells. Through the use of clotting factors purified from pigeon plasma, procoagulant activity could be detected with as few as 1-3 cells. Tissue factor antigen, detected through the use of immunogold labelling in conjunction with a polyclonal antibody which was highly specific to human tissue factor, was distributed uniformly over the plasma membrane of the endotoxin-stimulated cells. These studies suggest that this procoagulant activity might play an important role in the pathobiology of atherosclerosis in White Carneau pigeons by initiating fibrin polymerization and thus leading to thrombotic complications of the disease.

 

  • References

  • 1 Lewis JC, Taylor RG, Jerome WG. Foam cell characteristics in coronary arteries and aortas of White Cameau pigeons with moderate hypercholesterolemia. Ann NY Acad Sci 1985; 454: 091-100
    CrossrefPubMedSearch in Google Scholar
  • 2 Ross R. The pathogenesis of atherosclerosis: an update. N Engl J Med 1986; 326: 488-500
    PubMedSearch in Google Scholar
  • 3 Davies PF. Vascular cell interactions with special reference to pathogenesis of atherosclerosis. Lab Invest 1986; 55: 05-26
    PubMedSearch in Google Scholar
  • 4 Nawroth PP, Handley DA, Esmon CT, Stem DM. Interleukin-1 induces endothelial cell procoagulant while suppressing cell surface anticoagulant activity. Proc Natl Acad Sci 1986; 83: 3460-3464
    CrossrefPubMedSearch in Google Scholar
  • 5 Bevilacqua MP, Pober JS, Wheeler ME, Costran RS, Gimbrone MA. Interleukin-1 activation of vascular endothelium. Effect on procoagulant activity and leukocyte adhesion. Am J Pathol 1985; 121: 393-403
    PubMedSearch in Google Scholar
  • 6 Smith EB, Alexander KM, Massie IB. Insoluble fibrin in human aortic intima. Quantitation studies on the relationship between insoluble fibrin, soluble fibrinogen and low density lipoprotein. Atherosclerosis 1976; 23: 19-39
    CrossrefPubMedSearch in Google Scholar
  • 7 Smith EB. Fibrinogen, fibrin and fibrin degradation products in relation to atherosclerosis. In: Atherosclerosis VII. Fidge NH, Nestel PJ. eds Amsterdam: Elsevier Science Publishers BV; 1986: 459-462
    Search in Google Scholar
  • 8 Geczy CL. Induction of macrophage procoagulant by products of activated lymphocyte. Haemostasis 1984; 14: 400-411
    PubMedSearch in Google Scholar
  • 9 Semeraro N, Biondi A, Lorenzet R, Locati D, Mantovani A, Donati MB. Direct induction of tissue factor synthesis by endotoxin in human macrophages from diverse anatomical sites. Immunology 1983; 50: 529-535
    PubMedSearch in Google Scholar
  • 10 Semeraro N, Montemurro P, Conese M, Giordano D, Stella M, Restaino A, Cagnazzo G, Colucci M. Procoagulant activity of mononuclear phagozytes from different anatomical sites in patients with gynecological malignancies. Int J Cancer 1990; 45: 251-254
    CrossrefPubMedSearch in Google Scholar
  • 11 Lyberg T, Prydz H. Phorbol esters induce synthesis of thromboplastin activity in human monocytes. Biochem J 1981; 94: 699-706
    PubMedSearch in Google Scholar
  • 12 Semeraro N, DeLucia O, Lattanzio A, Montemurro P, Domenico G, Loizzi M, Carpagnano F. Procoagulant activity of human alveolar macrophages: different expression in patients with lung cancer. Int J Cancer 1986; 37: 525-529
    CrossrefPubMedSearch in Google Scholar
  • 13 Zeldis SM, Nemerson Y, Pitlick FA, Lentz TL. Tissue factor (thromboplastin): localization to plasma membranes by peroxidase-conjugated antibodies. Science 1972; 175: 766-768
    CrossrefPubMedSearch in Google Scholar
  • 14 Tipping PG, Malliaros J, Holdsworth SR. Procoagulant activity expression by macrophages from atheromatous vascular plaques. Atherosclerosis 1989; 79: 237-243
    CrossrefPubMedSearch in Google Scholar
  • 15 Wilcox JN, Smith KM, Schwartz SM, Gordon D. Localization of tissue factor in the normal vessel wall and in the atherosclerotic plaque. Proc Natl Acad Sci 1989; 86: 2839-2843
    CrossrefPubMedSearch in Google Scholar
  • 16 Edwards RL, Rickies FR. Macrophage procoagulants. In: Progress in Haemostasis and Thrombosis Vol. 7. Spact TH. ed. New York: Grune & Stratton; 1984: 183-201
    Search in Google Scholar
  • 17 Bach RR. Initiation of coagulation by tissue factor. CRC Crit Rev Biochem 1988; 23: 339-368
    PubMedSearch in Google Scholar
  • 18 Nemerson Y, Bach R. Tissue factor revisited. Prog Hemostas Thromb 1982; 6: 237-261
    PubMedSearch in Google Scholar
  • 19 Osterud B, Rapaport SI. Activation of factor IX by the reaction product of tissue factor and factor VII: additional pathway for initiating blood coagulation. Proc Natl Acad Sci USA 1977; 74: 5260-5264
    CrossrefPubMedSearch in Google Scholar
  • 20 Clarkson TB, Prichard RW, Netsky MG, Lofland HB. Atherosclerosis in pigeons. AMA Arch Pathol 1959; 68: 143-147
    PubMedSearch in Google Scholar
  • 21 Prichard RW, Clarkson TB, Goodman HO, Lofland HB. Aortic atherosclerosis in pigeons and its complications. AMA Arch Pathol 1964; 77: 244-257
    PubMedSearch in Google Scholar
  • 22 Jones NL, Gupta M, Deckelman C, Hantgan RR, Lewis JC. Monocyte/macrophages: a potential role in thrombosis secondary to atherosclerosis. Arteriosclerosis 1990; 10: A822
    PubMedSearch in Google Scholar
  • 23 Gupta M, Jones NL, Hantgan RR, Lewis JC. Pigeon monocyte derived macrophages express glycoproteins Ilb/IIIa. Arteriosclerosis 1990; 10: A822
    PubMedSearch in Google Scholar
  • 24 Jones NL, Allen NS, Lewis JC. βVLDL uptake by pigeon monocyte derived macrophages. Correlation of binding dynamics with 3dimensional ultrastructure. Cell Motil Cytoskel 1991; 19: 139-151
    CrossrefPubMedSearch in Google Scholar
  • 25 Jones NL, Lewis JC, Kilpatrick BA. Cytoskeletal disruption during human cytomegalovirus infection of human lung fibroblasts. Eur J Cell Biol 1986; 41: 304-312
    PubMedSearch in Google Scholar
  • 26 Doellgast GJ, Rothberger H. Enzyme-linked coagulation assay. II. A sensitive assay for tissue factor and factors II, VII, and XI. Anal Biochem 1986; 152: 199-207
    CrossrefPubMedSearch in Google Scholar
  • 27 Pitlick FA, Nemerson Y. Purification and characterization of tissue factor apoprotein. Meth Enzymol 1976; 45: 37-48
    PubMedSearch in Google Scholar
  • 28 Doellgast GJ. Enzyme-linked coagulation assay. IV. Sensitive sandwich enzyme-linked immunosorbent assays using Russell’s viper venom factor X activator-antibody conjugates. Anal Biochem 1987; 167: 097-105
    CrossrefPubMedSearch in Google Scholar
  • 29 Miletich JP, Broze Jr GJ, Majerus PW. The synthesis of sulphated dextran beads for isolation of human plasma coagulation factors II, IX and X. Anal Biochem 1980; 105: 304-310
    CrossrefPubMedSearch in Google Scholar
  • 30 Miletich JP, Broze Jr GJ, Majerus PW. Purification of human coagulation factors II, IX and X using sulphated dextran beads. In: Methods in Enzymology. Lorand L. ed. New York: Academic Press; 1981: 221-228
    Search in Google Scholar
  • 31 Broze GJ. Majerus PW Purification and properties of human coagulation factor VII. J Biol Chem 1980; 255: 1242-1247
    PubMedSearch in Google Scholar
  • 32 Hetland O, Brovold AB, Holme R, Gaudernack G, Prydz H. Thromboplastin (tissue factor) in plasma membranes of human monocytes. Biochem J 1985; 228: 735-743
    CrossrefPubMedSearch in Google Scholar
  • 33 Surprenant YM, Zuckerman SH. A novel microtiter plate assay for the quantitation of procoagulant activity on adherent monocytes, macrophage and endothelial cells. Thromb Res 1989; 53: 339-346
    CrossrefPubMedSearch in Google Scholar
  • 34 Prydz H, Allison AC. Tissue thromboplastin activity of isolated human monocytes. Thromb Haemostas 1978; 39: 582-591
    PubMedSearch in Google Scholar
  • 35 Schuff-Werner P, Claus G, Armstrong VW, Kostering H, Seidel D. Enhanced procoagulatory activity (PCA) of human monocytes/macrophages after in vitro stimulation with chemically modified LDL. Atherosclerosis 1989; 78: 109-112
    CrossrefPubMedSearch in Google Scholar
  • 36 Lyberg T, Gaidal KS, Evenson SA, Prydz H. Cellular cooperation in endothelial cell thromboplastin synthesis. Brit J Haematol 1983; 53: 85-95
    CrossrefPubMedSearch in Google Scholar
  • 37 Osterud B, Bjorklid E. The production and availability of tissue thromboplastin in cellular populations of whole blood exposed to various concentrations of endotoxin. An assay for detection of endotoxin. Scand J Haematol 1982; 29: 175-184
    PubMedSearch in Google Scholar
  • 38 Kaplan JE, Cardarelli PM, Rourke FJ, Weston LK, Moon DG, Blumenstock FA. Fibronectin augments binding of fibrin to macrophages. J Lab Clin Med 1989; 113: 168-176
    PubMedSearch in Google Scholar
  • 39 Owen J, Grossman BA, Palmer RH. Hyperlipidemia and in vivo hemostatic system activation. Sem Thromb Hemostas 1988; 14: 241-245
    Thieme ConnectPubMedSearch in Google Scholar
  • 40 Thompson WD, Smith EB. Atherosclerosis and the coagulation system. J Pathol 1989; 159: 097-106
    CrossrefPubMedSearch in Google Scholar
  • 41 Bini A, Fenoglio Jr J, Sobel J, Owen J, Fejgl M, Kaplan KL. Immunochemical characterization of fibrinogen, fibrin I and fibrin II in human thrombi and atherosclerotic lesions. Blood 1987; 69: 1038-1045
    PubMedSearch in Google Scholar