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Thromb Haemost 1997; 77(03): 585-590
DOI: 10.1055/s-0038-1656009
Vessel Wall
Schattauer GmbH Stuttgart

Haemostatic Properties of Human Pulmonary and Cerebral Microvascular Endothelial Cells

Georges E Grau
1   The Department of Anaesthesiology, Pharmacology and Surgical Intensive Care, Geneva, Switzerland
,
Philippe de Moerloose
2   Department of Division of Haemostasis, University Hospital and University Medical Centre, Geneva, Switzerland
,
Oana Bulla
2   Department of Division of Haemostasis, University Hospital and University Medical Centre, Geneva, Switzerland
,
Jinning Lou
1   The Department of Anaesthesiology, Pharmacology and Surgical Intensive Care, Geneva, Switzerland
,
Zheng Lei
1   The Department of Anaesthesiology, Pharmacology and Surgical Intensive Care, Geneva, Switzerland
,
Guido Reber
2   Department of Division of Haemostasis, University Hospital and University Medical Centre, Geneva, Switzerland
,
Nabil Mili
1   The Department of Anaesthesiology, Pharmacology and Surgical Intensive Care, Geneva, Switzerland
,
Bara Ricou
1   The Department of Anaesthesiology, Pharmacology and Surgical Intensive Care, Geneva, Switzerland
,
Denis R Morel
1   The Department of Anaesthesiology, Pharmacology and Surgical Intensive Care, Geneva, Switzerland
,
Peter M Suter
1   The Department of Anaesthesiology, Pharmacology and Surgical Intensive Care, Geneva, Switzerland
› Author Affiliations
Further Information

Publication History

Received 30 August 1996

Accepted after revision 14 October 1996

Publication Date:
11 July 2018 (online)

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Summary

Little is known on the haemostatic profiles of human microvascular endothelial cells (MVEC) from different tissues. In addition it is not known whether MVEC from patients display the same haemostatic pattern as MVEC coming from healthy controls. To address these questions MVEC from human lung and brain were isolated and stimulated with tumour necrosis factor α (TNF) and E. coli lipopolysaccharide (LPS) for 24 h. The level and the kinetics of procoagulant activity (PCA) and thrombomodulin (TM) expression were found to be different depending on the tissue of origin and on the agonist used. In particular, the inducible PCA was higher in lung than in brain MVEC, an observation that may be related to the frequency of lung involvement in septic shock. Differences were also observed for tissue plasminogen activator (t-PA) and plasminogen activator inhibitor 1 (PAI-1) with MVEC supernatants or cell lysates. These variables were then measured in lung MVEC purified from patients with acute respiratory distress syndrome (ARDS) and compared to controls. Cells from ARDS patients constitutively expressed more PCA and PAI-1 than controls. The fibrinolytic potential, expressed as t-PA/PAI-1 ratio, was lower in ARDS than in lung MVEC. It is concluded that MVEC display different haemostatic features depending on the tissue they come from and that lung MVEC from ARDS patients present a procoagulant profile when compared with those from controls.

 

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