Thrombosis and Haemostasis, Inhaltsverzeichnis Thromb Haemost 1984; 51(03): 388-391DOI: 10.1055/s-0038-1661108 Original Article Schattauer GmbH Stuttgart Immunocytochemical Localization of Albumin and Factor XIII in Thin Cryo Sections of Human Blood Platelets Autor*innen Institutsangaben Jan J Sixma 1 The Dept. of Haematology, State University Utrecht, The Netherlands Agnes van den Berg 2 The Center for Electron Microscopy, State University Utrecht, The Netherlands Marion Schiphorst 1 The Dept. of Haematology, State University Utrecht, The Netherlands Hans J Geuze 2 The Center for Electron Microscopy, State University Utrecht, The Netherlands Jan McDonagh 3 The Dept. of Pathology, Harvard Medical School and Beth Israel Hospital, Boston, Mass., U.S.A. Artikel empfehlen Abstract als PDF herunterladen(opens in new window) Artikel einzeln kaufen(opens in new window) Summary Albumin and factor XIIIa2 were localized in human blood platelets by immunofluorescence and protein A-colloidal gold immuno-electron microscopy using thin and ultrathin cryosections of mildly fixed human blood platelets. Albumin was found in α-granules, co-distributing with β-thromboglobulin in double label experiments. In platelets directly fixed in plasma, without previous washing, albumin was also localized on the plasma membrane and in the surface connecting system. Factor XIIIa2 was localized diffusely through the entire cytoplasmic matrix. Keywords KeywordsAlbumin - Factor XIII - α-granules - Immuno-electron microscopy PDF (325 kb) Referenzen References 1 Holmsen H, Weiss HJ. Secretable storage pools in platelets. Ann Rev med 1979; 30: 119-134 2 McDonagh J, McDonagh RP, Delage jr JM, Wagner RN. Factor XIII in human plasma and platelets. J Clin Invest 1969; 48: 940-946 3 White JG. Ultrastructural studies of the gray platelet syndrome. Am J Pathol 1979; 95: 445-462 4 Nurden AT, Kunicki ThJ, Dupuis D, Soria S. Specific protein and glycoprotein deficiencies in platelets isolated from two patients with the gray platelet syndrome. Blood 1982; 59: 709-718 5 Broekman MJ, Handin RI, Cohen Ph. Distribution of fibrinogen and platelets factor 4 and XIII in subcellular fractions of human platelet. Br J Haematol 1977; 31: 51-55 6 Lopaciuk S, Lovette KM, McDonagh J, Chiang HY K, McDonagh RP. Subcellular distribution of fibrinogen and factor XIII in human blood platelets. Thromb Res 1976; 8: 453-465 7 Bouma BN, de Graaf S, Slot JW, Zimmerman ThS. Human blood platelet factor VIII-related antigen: demonstration of release by α-chymotrypsin. Thromb Res 1979; 14: 687-696 8 Sander HJ, Slot JW, Bouma BN, Bolhuis PA, Pepper DS, Sixma JJ. Immuno-cytochemical localization of fibrinogen, platelet factor 4 and β-thromboglobulin in thin frozen sections of human blood platelets. J Clin Invest 1983; 72: 1277-1287 9 Akkermann JW N, Doucet de Bruine MH M, Gorter G, de Graaf S, Holme S, Lips JP M, Nijmeijer A, Over J, Starkenburg AE, Trieschnigg AM C, vd Veen J, Vlooswijk HA A, Wester J, Sixma JJ. Evaluation of platelets tests for measurement of cell integrity. Thromb Haemostas 1978; 39: 146-157 10 Towbin H, Staechelin T, Gordon J. Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Nat Acad Sci USA 1979; 76: 4350-4357 11 McDonagh J, Wagner WG, Hamilton EG, Hindenach BR, McDonagh RP. Affinity chromatography of human plasma and platelet factor XIII. Biochim Biophys Acta 1976; 446: 345-357 12 Skrzyma C, Reisner HM, McDonagh J. Characterization of the catalytic subunit of factor XIII by radioimmunoassay. Blood 1982; 60: 1089-1095 13 Hjelm H, Hjelm K, Sjøquist A. Protein A from staphylococcus aureus. Its isolation by affinity chromatography and its use as immunosorbent for isolation of immunoglobulins FEBS Lett 1972; 28: 73-76 14 Paul B, Niewiarowski S, Varma KG, Rucinski B, Rucker S, Lange E. Human platelet basic protein associated with antiheparin and antimitogenic activities, purification and partial characterization. Proc Natl Acad Sci USA 1980; 77: 5914-5918 15 Geuze HJ, Slot JW, Tokuyasu KT, Goedemans WE M, Griffith JM. Immunocytochemical localization of amylase and chymotrypsin in the exocrine pancreatic cell with special attention to the Golgi complex. J Cell Biol 1979; 82: 697-707 16 Geuze HJ, Slot JW. The subcellular localization of immunoglobulin in mouse plasma cells as studied with immunoferritin cytochemistry on ultrathin frozen sections. Am J Anat 1980; 158: 161-169 17 Slot JW, Geuze HJ. Sizing of protein A-colloidal gold probes for immunoelectron microscopy. J Cell Biol 1981; 90: 533-536 18 Geuze HJ, Slot JW, van der LeyP A, Scheffer RC T. Use of colloidal gold particles in double labelling immunoelectron microscopy of ultrathin frozen sections. J Cell Biol 1981; 89: 653-665 19 Tokuyasu KT. A study of positive staining of ultrathin frozen sections. J Ultrastruct Res 1978; 63: 287-307 20 Davey MB, Lüscher EF. Release reactions of human platelets induced by thrombin and other agents. Biochim Biophys Acta 1968; 165: 490-506 21 Bentfeld-Barker ME, Bainton DF. Identification of primary lysosomes in human megakaryocytes and platelets. Blood 1982; 39: 472-481 22 Mosher DF, Schad PE, Kleinman HK. Crosslinking of fibronectin to collagen by blood coagulation factor XIIIa . J Clin Invest 1979; 64: 781-787 23 Cohen J, Young-Bandale L, Blankenberg TA, Siefring GE, Bruner-Lorand J. Fibrinoligase-catalyzed crosslinking of myosin from platelets and skeletal muscle. Arch Biochem Biophys 1979; 192: 100-107 24 Mui PT K, Ganguly P. Cross linking of actin and fibrin by fibrin stabilizing factor. Am J Physiol 1977; 233: H346-H369 25 Wester J, Sixma JJ, Geuze JJ, van der Veen J. Morphology of the early hemostasis in human skin wounds. Lab Invest 1978; 39: 298-311
