Fibrinogen Fragments X, Y, D and E Increase Levels of Plasma Fibrinogen and Liver mRNAs Coding for Fibrinogen Polypeptides in Rats

H M G Princen; H J Moshage; J J Emeis; H J W de Haard; W Nieuwenhuizen; S H Yap

doi:10.1055/s-0038-1661276

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1985; 53(02): 212-215
DOI: 10.1055/s-0038-1661276

Original Article

Schattauer GmbH Stuttgart

Fibrinogen Fragments X, Y, D and E Increase Levels of Plasma Fibrinogen and Liver mRNAs Coding for Fibrinogen Polypeptides in Rats

H M G Princen

¹The Gaubius Institute, Health Research Division TNO, Leiden, The Netherlands

,

H J Moshage

The Division of Gastrointestinal and Liver Disease, Dept. of Medicine, St. Radboud Hospital, University of Nijmegen, Nijmegen, The Netherlands

,

J J Emeis

¹The Gaubius Institute, Health Research Division TNO, Leiden, The Netherlands

,

H J W de Haard

The Division of Gastrointestinal and Liver Disease, Dept. of Medicine, St. Radboud Hospital, University of Nijmegen, Nijmegen, The Netherlands

,

W Nieuwenhuizen

¹The Gaubius Institute, Health Research Division TNO, Leiden, The Netherlands

,

S H Yap

The Division of Gastrointestinal and Liver Disease, Dept. of Medicine, St. Radboud Hospital, University of Nijmegen, Nijmegen, The Netherlands

› Author Affiliations

Abstract

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Summary

Previously, we demonstrated that in vivo regulation of liver fibrinogen synthesis occurs via the fibrinogen mRNA level. However, the molecular regulatory mechanism of fibrinogen synthesis is still not well understood. Fibrinogen or fibrin degradation products might play an important role in regulating fibrinogen synthesis. In our present study, we have injected rats intraperitoneally with purified homologous fragments and measured the liver content of mRNA specific coding for fibrinogen. Increased levels of fibrinogen mRNA and elevated plasma fibrinogen concentrations were observed in rats after administration of fibrinogen degradation products X, Y, _DEGTA D_cate or E. Fragment E or E’ has a less stimulatory effect than X, Y or D_cate, whereas cross-linked fibrin degradation product D dimer does not increase fibrinogen synthesis. This article reports for the first time a stimulatory effect of the high molecular weight fibrinogen degradation products on fibrinogen synthesis.

Keywords

Fibrinogen mRNA - Fibrinogen synthesis - Fibrinogen fragments - Fibrin degradation products - Isolated hepatocytes

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References

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