Thromb Haemost 1983; 50(04): 838-843
DOI: 10.1055/s-0038-1665324
Original Article
Schattauer GmbH Stuttgart

Platelet Function, Size and Yield in Whole Blood and in Platelet-Rich Plasma Prepared Using Differing Centrifugation Force and Time in Domestic and Food-Producing Animals

R M Clemmons
*   The Department of Medical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, WA, U.S.A.
,
E L Bliss
**   The Department of Preventive Medicine, College of Veterinary Medicine, University of Florida, Gainesville, WA, U.S.A.
,
M R Dorsey-Lee
*   The Department of Medical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, WA, U.S.A.
,
C L Seachord
***   The Laboratory of Comparative Thrombosis and Hemostasis, College of Veterinary Medicine, Washington State University, Pullman, WA, U.S.A.
,
K M Meyers
***   The Laboratory of Comparative Thrombosis and Hemostasis, College of Veterinary Medicine, Washington State University, Pullman, WA, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 10 March 1983

Accepted 29 September 1983

Publication Date:
18 July 2018 (online)

Summary

The effects of centrifugation force and time upon platelets function, mean platelet volume and platelet yield were compared with whole blood platelet counts and size in citrated blood samples from the bovine, canine, caprine, equine, feline, ovine and porcine species. The results were similar, for a given species, irregardless of sample volume. Bovine, caprine, feline and ovine platelet yields and mean platelet volumes were maximal when platelet-rich plasma was prepared using longer centrifugation times and lower gravitational forces. Canine, equine and porcine platelet yields and mean platelet volumes were maximal when platelet-rich plasma was prepared using shorter centrifugation times and higher gravitational forces. Platelet aggregation to adenosine diphosphate or arachidonic add was not effected by the method of platelet-rich plasma preparation in bovine, caprine, feline, ovine or pordne platelets. Equine platelet aggregation was maximal when platelet-rich plasma was prepared using longer centrifugation times and lower gravitational forces. Canine platelet aggregation, particularly arachidonic add-induced aggregation, was maximal when platelet-rich plasma was prepared using short centrifugation times and higher gravitational forces. It appeared that the effects of centrifugation parameters upon platelet yield depended upon the relative difference between platelet and red blood cell volumes.

 
  • References

  • 1 Frojmovic MM, Milton JG. Human platelet size, shape, and related functions in health and disease. Physiol Rev 1982; 62: 185-261
  • 2 Dodds JW. Second International Registry of Animal Models of Thrombosis and Hemorrhagic Disease. National Academic Press; Washington, D. C: 1981
  • 3 Meyers KM, Katz JB, Clemmons RM, Smith JB, Holmsen H. An evaluation of the arachidonate pathway of platelets from companion and food-producing animals, mink, and man. Thromb Res 1980; 20: 13-24
  • 4 Dodds JW. Platelet function in animals: species specificity. In: Platelets: A Multidisciplinary Approach. de Gaetano G, Garattini G. (eds) Raven Press; New York: 1978. pp 239-258
  • 5 Clemmons RM, Meyers KM. Acquisition and aggregation of canine platelets: basic mechanisms of function and differences because of breed origin. J Am Vet Res in press
  • 6 Calkins J, Lane KP, Losasso B, Thurber LE. Comparative study of platelet aggregation in various species. J Med 1974; 5: 292-296
  • 7 Donner L, Houstkova J. Some properties of blood platelets in animal species. Folia Haematol 1972; 98: 296-302
  • 8 Sinakos Z, Caen JP. Platelet aggregation in mammalians. A compara-tive study Thromb Haemostas 1967; 17: 99-111
  • 9 Meyers KM, Holmsen H, Seachort CL. Comparative study of dense granule constituents. Am J Physiol 1982; 243: R454-R461
  • 10 Prost-Dvojakovic RJ, Le Tohic F, Boulard CH. Study of platelet volumes and diameters in 11 mammals. In: Platelets: Recent Advan-ces in Basic Research and Clinical Aspects. Ulutin ON, Jones JW. (eds) American Elsevier Publishing Co Inc; New York: 1975. pp 30-36
  • 11 Ross DW, Ayscue L, Gulley M. Automated platelet counts: accuracy, precision, and range. Am J Clin Pathol 1980; 74: 151-156
  • 12 Evans VJ, Glasser L. Accuracy of low electronic platelet counts using platelet distribution curves. Am J Med Technol 1981; 47: 15-18
  • 13 Bessman JD. Evaluation of automated whole-blood platelet counts and particle sizing. Am J Clin Pathol 1980; 74: 157-162
  • 14 Heinemann G, Gunner H. Thrombozytenzählung in Vollblut mit einem neuen Zählgerät mit hydrodynamisch fokussierter Kammer. Blut 1980; 40: 275-283
  • 15 Mehta P, Mehta J, Pepine CJ. Influence of the normal forearm vascular bed on platelet aggregation, counts, and size. Microvas Res 1981; 21: 229-233
  • 16 Kelton JG, Neame PB, Bishop J, Ali M, Gauldie J, Hirsh J. The direct assay for platelet-associated IgG (PAIgG): lack of association between antibody level and platelet size. Blood 1979; 53: 73-80
  • 17 Nelson RB, Kehl D. Electronically determined platelet indices in thrombocytopenic patients. Cancer 1981; 48: 954-956
  • 18 McLean MR, Hause LL. Collagen-induced platelet aggregation as studied by electronic particle size analysis. Thromb Haemostas 1981; 46: 731-733
  • 19 Cortelazzo S, Barbui T, Bassan R, Dini E. Abnormal aggregation and increased size of platelets in myeloproliferative disorders. Thromb Haemostas 1980; 43: 127-130
  • 20 Haver VM, Gear AR L. Functional fractionation of platelets. J Lab Clin Med 1981; 97: 187-204
  • 21 Karpatkin S. Heterogeneity of rabbit platelets. VI. Further resolution of changes in platelet density, volume, and radioactivity following cohort labelling with 75Se-selenomethione. Br J Haematol 1978; 39: 459-469
  • 22 Rand ML, Greenberg JP, Packman MA, Mustard JF. Density subpopulations of rabbit platelets: size, protein, and sialic acid content, and specific radioactivity changes following labeling with35S- sulfate in vivo. Blood 1981; 57: 741-746