Medicinal Signaling Cells Metabolite Oral Based as a Potential Biocompatible Biomaterial Accelerating Oral Ulcer Healing (In Vitro Study)

Abstract

Objective Medicinal signaling cells metabolite (MSCM) is often considered medical waste even though it contains abundant growth factors, and advantageous micro- and macromolecules that can accelerate healing in oral ulcer.

The purpose of this experimental laboratory study was to analyze the biocompatibility and potential of MSCM, (oral based) to accelerate healing in oral ulcer (in vitro).

Materials and Methods MSCM (oral based) was obtained by mixing 10 mL of MSCM and 2% of carboxymethyl cellulose sodium. 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (or MTT assay) was obtained using human gingival somatic cell culture to examine cell viability treated with MSCM (oral based). Fourier transform infrared spectroscopy was performed to know the functional structure and composition of MSCM (oral based). To know the elemental composition of MSCM (oral based), energy-dispersive X-ray analysis was performed. Scratch test was performed to know the ability of MSCM (oral based) to increase human somatic cell proliferation.

Results MSCM (oral based) has good cell viability. MSCM (oral based) administration accelerated the proliferation of human somatic cell culture after 12-hours in vitro. MSCM (oral based) has carboxylic acids and derivatives chemical bond. MSCM (oral based) mostly contained carbon and potassium but did not contain heavy metal substances.

Conclusions MSCM (oral based) has a biocompatible and potential ability to accelerate healing in oral ulcer in vitro. It would be useful in daily clinical practice in treating traumatic oral ulcer.

Keywords

medicinal signaling cells - wound healing - biomaterial - metabolite - biocompatibility

• References

• 1 Rennick LA, Campbell PM, Naidu A, Taylor RW, Buschang PH. Effectiveness of a novel topical powder on the treatment of traumatic oral ulcers in orthodontic patients: a randomized controlled trial. Angle Orthod 2016; 86 (03) 351-357
• 2 Khan RMS, Hassan KR, Rizwan M. Prevalence and type of oral mucosal lesion in patient with fixed orthodontic appliances. Ashraf J Med Forum 2016; 27 (04) 12-16
• 3 Kvam E, Bondevik O, Gjerdet NR. Traumatic ulcers and pain in adults during orthodontic treatment. Community Dent Oral Epidemiol 1989; 17 (03) 154-157
• 4 Puspasari A, Harijanti K, Soebadi B, Hendarti HT, Radithia D, Ernawati DS. Effects of topical application of propolis extract on fibroblast growth factor-2 and fibroblast expression in the traumatic ulcers of diabetic Rattus norvegicus . J Oral Maxillofac Pathol 2018; 22 (01) 54-58
• 5 Ernawati DS, Puspa A. Expression of vascular endothelial growth factor and matrix metalloproteinase-9 in Apis mellifera Lawang propolis extract gel-treated traumatic ulcers in diabetic rats. Vet World 2018; 11 (03) 304-309
• 6 Kusumaningsih T, Subijanto MS, Indrawati R, Devijanti RR. The level of beta defensin-2 in saliva and its expression in parotid gland epithelial cells after probiotic (Lactobacillus reuteri) induction to inhibit Streptococcus mutans in caries. Eur J Dent 2016; 10 (04) 556-560
• 7 Jiang Y, Magli L, Russo M. Bacterium-dependent induction of cytokines in mononuclear cells and their pathologic consequences in vivo. Infect Immun 1999; 67 (05) 2125-2130
• 8 Descroix V, Coudert AE, Vigé A. et al. Efficacy of topical 1% lidocaine in the symptomatic treatment of pain associated with oral mucosal trauma or minor oral aphthous ulcer: a randomized, double-blind, placebo-controlled, parallel-group, single-dose study. J Orofac Pain 2011; 25 (04) 327-332
• 9 Karavana Hizarcioğlu SY, Sezer B, Güneri P. et al. Efficacy of topical benzydamine hydrochloride gel on oral mucosal ulcers: an in vivo animal study. Int J Oral Maxillofac Surg 2011; 40 (09) 973-978
• 10 Apriasari ML, Rahayu RP, Ernawati DS. Mauli banana stem extract application increased expression of NFKB in traumatic ulcer healing. Majalah Kedokteran Gigi 2018; 51 (02) 62-70
• 11 Caplan AI. Mesenchymal stem cells: time to change the name!. Stem Cells Transl Med 2017; 6 (06) 1445-1451
• 12 Somoza RA, Correa D, Caplan AI. The role of mesenchymal stem cells as medicinal signaling cells. Nature protocol and recipe for researchers. Nat Protoc 2017; 11 (01) 1
• 13 Tanabe S. Role of mesenchymal stem cells in cell life and their signaling. World J Stem Cells 2014; 6 (01) 24-32
• 14 Rantam FA, Ferdiansyah F, Purwati P. Mesenchymal Stem cell, Hematopoietic Stem Cells, and Application Model. 2nd. ed. Surabaya: Airlangga University Press; 2015
• 15 Prahasanti C, Wulandari DT, Ulfa N. Viability test of fish scale collagen (Oshpronemus gouramy) on baby hamster kidney fibroblasts-21 fibroblast cell culture. Vet World 2018; 11 (04) 506-510
• 16 Nugraha AP, Rantam FA, Ernawati DS. et al. Gingival mesenchymal stem cells from Wistar rat’s gingiva (Rattus norvegicus) isolation and characterization (in vitro study). J Int Dent Med Res 2018; 11 (02) 694-699
• 17 Nugraha AP, Narmada IB, Ernawati DS. et al. Somatic cells acceleration by platelet rich fibrin. Indian Vet J 2019; 96 (04) 30-34
• 18 Nugraha AP, Narmada IB, Ernawati DS. et al. In vitro bone sialoprotein-I expression in combined gingival stromal cells and platelet rich fibrin during osteogenic differentiation. Trop J Pharm Res 2018; 17 (12) 2341-2345
• 19 Nugraha AP, Narmada IB, Ernawati DS. et al. Bone alkaline phosphatase and osteocalcin expression of rat’s Gingival mesenchymal stem cells cultured in platelet-rich fibrin for bone remodeling (in vitro. study). Eur J Dent 2018; 12 (04) 566-573
• 20 Nugraha AP, Narmada IB, Ernawati DS. et al. Osteogenic potential of gingival stromal progenitor cells cultured in platelet rich fibrin is predicted by core-binding factor subunit-α1/Sox9 expression ratio (in vitro). F1000 Res 2018; 7: 1134
• 21 Nugraha AP, Narmada IB, Ernawati DS. et al. The Aggrecan expression post platelet rich fibrin administration in gingival medicinal signaling cells in Wistar rats (Rattus norvegicus) during the early osteogenic differentiation (in vitro). Kafkas Univ Vet Fak Derg 2019; 25 (03) 421-425
• 22 Lai RC, Yeo RWY, Tan SS, Zhang B, Yin Y, Sze NSK. Mesenchymal Stem Cell Exosomes: The Future MSC Based Therapy? Mesenchymal Stem Cell Therapy. Springer; 2013: >39-61
• 23 Carvalho MM, Teixeira FG, Reis RL, Sousa N, Salgado AJ. Mesenchymal stem cells in the umbilical cord: phenotypic characterization, secretome and applications in central nervous system regenerative medicine. Curr Stem Cell Res Ther 2011; 6 (03) 221-228
• 24 Bernardo ME, Fibbe WE. Mesenchymal stromal cells: sensors and switchers of inflammation. Cell Stem Cell 2013; 13 (04) 392-402
• 25 Caplan AI, Correa D. The MSC: an injury drugstore. Cell Stem Cell 2011; 9 (01) 11-15
• 26 Caplan AI, Sorrell JM. The MSC curtain that stops the immune system. Immunol Lett 2015; 168 (02) 136-139
• 27 Vazquez-Zapien GJ, Mata-Miranda MM, Sanchez-Monroy V, Delgado-Macuil RJ, Perez-Ishiwara DG, Rojas-Lopez M. FTIR spectroscopic and molecular analysis during differentiation of pluripotent stem cells to pancreatic cells. Stem Cells Int 2016; 2016: 6709714
• 28 Scimeca M, Bischetti S, Lamsira HK, Bonfiglio R, Bonanno E. Energy dispersive X-ray (EDX) microanalysis: a powerful tool in biomedical research and diagnosis. Eur J Histochem 2018; 62 (01) 2841
• 29 Singh R, Gautam N, Mishra A, Gupta R. Heavy metals and living systems: an overview. Indian J Pharmacol 2011; 43 (03) 246-253