PDF herunterladen
CC BY-NC-ND 4.0 · Eur J Dent 2019; 13(02): 206-212
DOI: 10.1055/s-0039-1694305
Original Article
Dental Investigation Society

Osteogenic Differentiation and Biocompatibility of Bovine Teeth Scaffold with Rat Adipose-derived Mesenchymal Stem Cells

Desi Sandra Sari
1   Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
2   Department of Periodontics, Faculty of Dentistry, Universitas Jember, Jember, Indonesia
,
Ernie Maduratna
3   Department of Periodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ferdiansyah,
Fourier Dzar Eljabbar Latief
6   Micro-CT Laboratory, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung, Indonesia
,
Satuman,
Alexander Patera Nugraha
1   Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
5   Stem Cells Research and Development Center, Universitas Airlangga, Surabaya, Indonesia
8   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ketut Sudiana
9   Department of Microscope Electron, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
10   Department of Pathology Anatomy, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Fedik Abdul Rantam
5   Stem Cells Research and Development Center, Universitas Airlangga, Surabaya, Indonesia
11   Laboratory of Virology, Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
› Institutsangaben Funding The research was funded by doctoral dissertation research, Direktorat Jenderal Penguatan Riset dan Pengembangan, The Ministry of Research, Technology and Higher Education of The Republic of Indonesia (Kemenristekdikti RI letter of appointment agreement number 058.SP2H/LT/DRPM/2018.
Weitere Informationen

Publikationsverlauf

Publikationsdatum:
16. September 2019 (online)

Auch verfügbar auf
   Lizenzen und Reprints

Abstract

Objective Adipose-derived mesenchymal stem cells (ADMSCs) have great potential for regenerative medicine. These have been combined with biomaterials such as Bovine teeth that are preferred as a periodontal regeneration material. The main purpose of this study is to evaluate and analyze a biocompatibility test and osteogenic differentiation of bovine teeth scaffold seeded with ADMSCs in vitro.

Materials and Methods A true experimental study with post-test only group design was conducted. Random sampling and Lameshow's formula were used to determine the sample. The scaffold, obtained from bovine teeth as the bone graft material, was analyzed using 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and its attachment was evaluated by scanning electron microcopy (SEM) and micro-computed tomography with ADMSCs. ADMDSCs attachment present in the bovine teeth scaffold was assessed using SEM at 1-hour, 12-hour, and 24-hour intervals.

Statistical Analysis Analysis of variance was used to analyze the MTT assay results (p < 0.05) based on normality and homogeneity test (p > 0.05).

Results The highest viability of cells (97.08%) was found at a concentration of 10% by means of an MTT test (p < 0.05). The results of three-dimensional bovine teeth scaffold showed the average particle size to be 500 µm. ADMSCs cell attachment to the scaffold bovine teeth showed a significant increase in the number of cells attached after 24 hours compared with those at 1 and 12 hours. Alizarin red staining showed an increase in ADMSC osteogenic differentiation after it was combined with bovine teeth scaffold.

Conclusion The scaffold from bovine teeth is biocompatible and accelerates osteogenic differentiation of ADMSC.

Keywords

adipose-derived mesenchymal stem cells - bovine teeth - scaffold - tissue engineering
 

  • References

  • 1 Amal SP, Yash M, Preeti N. Tissue engineering of craniofacial tissues—a review. Regen Med Tissue Eng 2013; 2 (06) 1-19
    Suche in Google Scholar
  • 2 Siddhartha VA, Shartak B, Rashmi G, Shilpa S, Uzma B. Tissue engineering in periodontics—a novel therapy. Ann Dental Res 2012; 2 (01) 01-07
    Suche in Google Scholar
  • 3 Young-Kyun K. Bone graft material using teeth. J Korean Assoc Oral Maxillofac Surg 2012; 38: 134-138
    CrossrefSuche in Google Scholar
  • 4 Teruel JdeD, Alcolea A, Hernández A, Ruiz AJ. Comparison of chemical composition of enamel and dentine in human, bovine, porcine and ovine teeth. Arch Oral Biol 2015; 60 (05) 768-775
    CrossrefPubMedSuche in Google Scholar
  • 5 de Oliveira GS, Miziara MN, Silva ER, Ferreira EL, Biulchi APF, Alves JB. Enhanced bone formation during healing process of tooth sockets filled with demineralized human dentine matrix. Aust Dent J 2013; 58 (03) 326-332
    CrossrefPubMedSuche in Google Scholar
  • 6 Amini AR, Laurencin CT, Nukavarapu SP. Bone tissue engineering: recent advances and challenges. Crit Rev Biomed Eng 2012; 40 (05) 363-408
    CrossrefPubMedSuche in Google Scholar
  • 7 Koga T, Minamizato T, Kawai Y. et al. Bone regeneration using dentin matrix depends on the degree of demineralization and particle size. PLoS One 2016; 11 (01) e0147235
    CrossrefPubMedSuche in Google Scholar
  • 8 Monthira S. Dentin as bone graft substitution. Songklanakarin Dent J 2014; 2 (01) 21-27
    Suche in Google Scholar
  • 9 Kim YK, Lee J, Um IW. et al. Tooth-derived bone graft material. J Korean Assoc Oral Maxillofac Surg 2013; 39 (03) 103-111
    CrossrefPubMedSuche in Google Scholar
  • 10 Nassif L, El Sabban M. Mesenchymal stem cells in combination with scaffolds for bone tissue engineering. Materials (Basel) 2011; 4 (10) 1793-1804
    CrossrefPubMedSuche in Google Scholar
  • 11 Baer PC, Werner L. Adipose-derived stromal/stem cells and their differentiation potential into the endothelial lineage. In: Jurgen H, Erhard H. eds. Adult and Pluripotent Stem Cells. Köln, Germany: Springer; 2014: 53-64
    CrossrefSuche in Google Scholar
  • 12 Lotfy A, Salama M, Zahran F, Jones E, Badawy A, Sobh M. Characterization of mesenchymal stem cells derived from rat bone marrow and adipose tissue: a comparative study. Int J Stem Cells 2014; 7 (02) 135-142
    CrossrefPubMedSuche in Google Scholar
  • 13 Dominici M, Le Blanc K, Mueller I. et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006; 8 (04) 315-317
    CrossrefPubMedSuche in Google Scholar
  • 14 Chiho I, Ken S. Mesenchymal Stem Cells for Regenerative Therapy: Optimization of Cell Preparation Protocols. Hindawi Publishing Corporation BioMed Research International. 2014: 1-11
    PubMedSuche in Google Scholar
  • 15 Ibrahim H, Keyvan M, Ian MB, Patrıcio JON, Luiz AS. Evaluation of Osteoconductive and Osteogenic Potential of a Dentin-Based Bone Substitute Using a Calvarial Defect Model. Hindawi Publishing Corporation International Journal of Dentistry. 2012: 1-6
    PubMedSuche in Google Scholar
  • 16 George JP, Chakravarty P, Chowdhary KY, Purushothama H, Rao JA. Attachment and differentiation of human umbilical cord stem cells on to the tooth root surface with and without the use of fibroblast growth factor-an in vitro study. Int J Stem Cells 2015; 8 (01) 90-98
    CrossrefPubMedSuche in Google Scholar
  • 17 Rada T, Gomes ME, Reis RL. A novel method for the isolation of subpopulations of rat adipose stem cells with different proliferation and osteogenic differentiation potentials. J Tissue Eng Regen Med 2011; 5 (08) 655-664
    CrossrefPubMedSuche in Google Scholar
  • 18 Kamal D, Iskandriati I, Dilogo N. et al. Comparison of culture mesenchymal stem cell derived from bone marrow or peripheral blood of rats. J Exp Integr Med 2014; 4 (01) 17-22
    CrossrefSuche in Google Scholar
  • 19 Martens W, Wolfs E, Struys T, Politis C, Bronckaers A, Lambrichts I. Expression pattern of basal markers in human dental pulp stem cells and tissue. Cells Tissues Organs 2012; 196 (06) 490-500
    CrossrefPubMedSuche in Google Scholar
  • 20 Birmingham E, Niebur GL, McHugh PE, Shaw G, Barry FP, McNamara LM. Osteogenic differentiation of mesenchymal stem cells is regulated by osteocyte and osteoblast cells in a simplified bone niche. Eur Cell Mater 2012; 23: 13-27
    CrossrefPubMedSuche in Google Scholar
  • 21 Nather YN, Hilmi N. Allograft Procurement Processing and Transplantation: A Comprehensive Guide for Tissue Bank. Singapore: World Scientific Publishing Co. Pvt Ltd.; 2010: 356-358
    Suche in Google Scholar
  • 22 Ola SM. Preparation and characterization of hydroxyapatite from bovine teeth. Nat Appl Sci 2017; 11 (08) 623-630
    Suche in Google Scholar
  • 23 David BK, Purwati, Fedik AR, Ferdiansyah. Coen DP. Healing mechanism and osteogenic capacity of bovine bone mineral-human amniotic mesenchymal stem cell and autogenous bone graft in critical size mandibular defect. J Biomed Sci Eng 2015; 8: 733-746
    CrossrefSuche in Google Scholar
  • 24 Yaling S, Jerry NR, Adrain SB, Simon B, Brend LA. Adipose-derived stem cells combined with a demineralized cancellous bone substrate for bone regeneration. Tissue Eng 2012; 18 (13) 1-9
    Suche in Google Scholar
  • 25 Jin GZ, Park JH, Wall I, Kim HW. Isolation and culture of primary rat adipose derived stem cells using porous biopolymer microcarriers. Tissue Eng Regen Med 2016; 13 (03) 242-250
    CrossrefPubMedSuche in Google Scholar
  • 26 Seyed JH, Hamed G, Ahmad G, Hamid RS, Majid GM, Elaheh M. Isolation characterization and differentiation of rat adipose tissue derived mesenchymal stem cells. Int J Pediatr 2014; 2 (Suppl. 03) 1-6
    Suche in Google Scholar
  • 27 Uccelli A, Moretta L, Pistoia V. Mesenchymal stem cells in health and disease. Nat Rev Immunol 2008; 8 (09) 726-736
    CrossrefPubMedSuche in Google Scholar
  • 28 Cao F, Liu T, Xu Y, Xu D, Feng S. Culture and properties of adipose-derived mesenchymal stem cells: characteristics in vitro and immunosuppression in vivo. Int J Clin Exp Pathol 2015; 8 (07) 7694-7709
    PubMedSuche in Google Scholar
  • 29 Kawashima N. Characterisation of dental pulp stem cells: a new horizon for tissue regeneration?. Arch Oral Biol 2012; 57 (11) 1439-1458
    CrossrefPubMedSuche in Google Scholar
  • 30 Hassan IHE, Mohamed AS, Soad AK, Omnia KRE. Adipose derived mesenchymal stem cell differentiation into adipogenic and osteogenic stem cells. Stud Stem Cells Res Ther 2011; 2 (01) 17-24
    Suche in Google Scholar
  • 31 Locke M, Windsor J, Dunbar PR. Human adipose-derived stem cells: isolation, characterization and applications in surgery. ANZ J Surg 2009; 79 (04) 235-244
    CrossrefPubMedSuche in Google Scholar
  • 32 Li TX, Yuan J, Chen Y. et al. Differentiation of mesenchymal stem cells from human umbilical cord tissue into odontoblast-like cells using the conditioned medium of tooth germ cells in vitro. BioMed Res Int 2013; 2013: 1-10
    CrossrefPubMedSuche in Google Scholar
  • 33 Sundelacruz S, Kaplan DL. Stem cell- and scaffold-based tissue engineering approaches to osteochondral regenerative medicine. Semin Cell Dev Biol 2009; 20 (06) 646-655
    CrossrefPubMedSuche in Google Scholar
  • 34 Thitiset T, Damrongsakkul S, Bunaprasert T, Leeanansaksiri W, Honsawek S. Development of collagen/demineralized bone powder scaffolds and periosteum-derived cells for bone tissue engineering application. Int J Mol Sci 2013; 14 (01) 2056-2071
    CrossrefPubMedSuche in Google Scholar
  • 35 da Cruz GA, de Toledo S, Sallum EA, de Lima AF. Morphological and chemical analysis of bone substitutes by scanning electron microscopy and microanalysis by spectroscopy of dispersion energy. Braz Dent J 2007; 18 (02) 129-133
    PubMedSuche in Google Scholar
  • 36 Chen Q, Shou P, Zheng C. et al. Fate decision of mesenchymal stem cells: adipocytes or osteoblasts?. Cell Death Differ 2016; 23 (07) 1128-1139
    CrossrefPubMedSuche in Google Scholar
  • 37 Darby I. Periodontal materials. Aust Dent J 2011; 56 (Suppl. 01) 107-118
    PubMedSuche in Google Scholar
  • 38 Hidetoshi S, Eiji N, Hiroshi S, Masatsugu S. Possible functional scaffold for periodontal regeneration. Jpn Dent Sci Rev 2013; 49: 118-130
    CrossrefSuche in Google Scholar
  • 39 Li Z, Yossy M, Yanging W, Yayou L, Seeram R. Review scaffold design and stem cells for tooth regeneration. Jpn Dent Sci Rev 2013; 9: 14-26
    Suche in Google Scholar
  • 40 Weiguang W, Guilherme C, William SA. et al. Enhancing the hydrophilicity and cell attachment of 3D printed PCL/graphene scaffold for bone tissue engineering. Materials (Basel) 2016; 9 (992) 1-10
    Suche in Google Scholar
  • 41 Supronowicz P, Gill E, Trujillo A. et al. Human adipose-derived side population stem cells cultured on demineralized bone matrix for bone tissue engineering. Tissue Eng Part A 2011; 17 (05) (06) 789-798
    PubMedSuche in Google Scholar