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CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(02): 228-235
DOI: 10.1055/s-0040-1719215
Original Article

The Effect of Anadara granosa Shell’s–Stichopus hermanni Scaffold on CD44 and IL-10 Expression to Decrease Osteoclasts in Socket Healing

Rima Parwati Sari
1   Department of Oral Biology, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
,
Syamsulina Revianti
1   Department of Oral Biology, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
,
Dwi Andriani
1   Department of Oral Biology, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
,
Widyasri Prananingrum
2   Department of Biomaterial, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
,
Retno Pudji Rahayu
3   Department of Oral Pathology and Maxilofacial, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Sri Agus Sudjarwo
4   Department of Pharmacology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
› Author Affiliations Funding This study was supported by the National Research Program Grant (120/SP2H/LT/DRPM/IV/2017) and funded by the Indonesian Ministry of Research Technology and Higher Education.
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Abstract

Objectives This article aimed to investigate the effect of Anadara granosa (AG) shell’s–Stichopus hermanni scaffold on cluster of differentiation (CD)44 and interleukin-10 (IL-10) expression to decrease osteoclasts in socket healing.

Materials and Methods Thirty male Wistar rats were divided into five groups. The lower left incisor was extracted, then given a placebo for group control (K), the treatment group was administered with scaffold from AG shells, and a treatment group with scaffold from blood cockle shell–S. hermanni with the concentration of 0.4, 0.8, and 1.6% (AGSH0.4; AGSH0.8; AGSH1.6). We made a bone graft from a combination of AGSH extract using the freeze-dried method. The socket was sutured by silk braid immediately. Third and Seventh days postextraction, animals are killed. CD44 and IL-10 expression were examined with immunohistochemistry, as well as osteoclast was examined with hematoxylin-eosin.

Statistical Analysis The data were analyzed using a one-way analysis of variance (for CD44 and osteoclast) and Kruskal–Wallis’ test (for IL-10) followed by a post hoc test in which the result of p < 0.05.

Results Scaffold from a combination of AGSH increased CD44 expression significantly, which enhanced IL-10 expression thereby decreased the number of osteoclasts in socket healing on days 3 and 7.

Conclusion Scaffold of AG shell–S. hermanni with a concentration of 0.8% was effective to enhance CD44 and IL-10 expression to decrease osteoclast in socket healing after tooth extraction.

Keywords

Anadara granosa shell - Stichopus hermanni - hyaluronic acid - CD44 - IL-10 - osteoclast - socket healing


Publication History

Article published online:
28 January 2021

© 2021. European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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