Subscribe to RSS
Please copy the URL and add it into your RSS Feed Reader.
https://www.thieme-connect.de/rss/thieme/en/10.1055-s-00042133.xml
Download PDF
CC BY 4.0 · Eur J Dent 2024; 18(02): 501-509
DOI: 10.1055/s-0043-1772699
DOI: 10.1055/s-0043-1772699
Original Article
Gingiva Mesenchymal Stem Cells Normoxic or Hypoxic Preconditioned Application Under Orthodontic Mechanical Force on Osterix, Osteopontin, and ALP Expression
Abstract
Objectives The aim of this article was to investigate Osterix, ALP, and osteopontin expression in the compression and tension sides of alveolar bone after the application of normoxic/hypoxic-preconditioned GMSCs in rabbits (Oryctolagus cuniculus) induced with OMF.
Materials and Methods Forty-eight healthy, young male rabbits were divided into four groups: [-] OMF; [+] OMF; OMF with GMSCs normoxic-preconditioned; and OMF and GMSCs hypoxic-preconditioned. The central incisor and left mandibular molar in the experimental animals were moved, the mandibular first molar was moved mesially using nickel titanium (NiTi) and stainless steel ligature wire connected to a 50 g/mm2 light force closed coil spring. Allogeneic application of normoxic or hypoxic-preconditioned GMSCs was used in as many as 106 cells in a 20 µL phosphate buffered saline single dose and injected into experimental animals' gingiva after 1 day of OTM. On days 7, 14, and 28, all experimental animals were euthanized. Osterix, ALP, and osteopontin expressions were examined by immunohistochemistry.
Results Osterix, ALP, and osteopontin expressions were significantly different after allogeneic application of hypoxic-preconditioned GMSCs than normoxic-preconditioned GMSCs in the tension and compression of the alveolar bone side during OMF (p < 0.05).
Conclusion Osterix, ALP, and osteopontin expressions were significantly more enhanced post-transplantation of GMSCs with hypoxic-preconditioning than after transplantation of normoxic-preconditioned GMSCs in rabbits (O. cuniculus) induced with OMF.
Publication History
Article published online:
23 November 2023
© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India
-
References
- 1 Narmada IB, Rubianto M, Putra ST. The role of low-intensity biostimulation laser therapy in transforming growth factor β1, bone alkaline phosphatase and osteocalcin expression during orthodontic tooth movement in Cavia porcellus. Eur J Dent 2019; 13 (01) 102-107
- 2 Narmada IB, Putri PD, Lucynda L, Triwardhani A, Ardani IGAW, Nugraha AP. Effect of caffeic acid phenethyl ester provision on fibroblast growth factor-2, matrix metalloproteinase-9 expression, osteoclast and osteoblast numbers during experimental tooth movement in Wistar rats (Rattus norvegicus). Eur J Dent 2021; 15 (02) 295-301
- 3 Meikle MC. The tissue, cellular, and molecular regulation of orthodontic tooth movement: 100 years after Carl Sandstedt. Eur J Orthod 2006; 28 (03) 221-240
- 4 Will LA. Orthodontic tooth movement: a historic prospective. Front Oral Biol 2016; 18: 46-55
- 5 Asiry MA. Biological aspects of orthodontic tooth movement: a review of literature. Saudi J Biol Sci 2018; 25 (06) 1027-1032
- 6 Tsichlaki A, Chin SY, Pandis N, Fleming PS. How long does treatment with fixed orthodontic appliances last? A systematic review. Am J Orthod Dentofacial Orthop 2016; 149 (03) 308-318
- 7 Mavreas D, Athanasiou AE. Factors affecting the duration of orthodontic treatment: a systematic review. Eur J Orthod 2008; 30 (04) 386-395
- 8 Miles P. Accelerated orthodontic treatment - what's the evidence?. Aust Dent J 2017; 62 (Suppl. 01) 63-70
- 9 Khojasteh A, Motamedian SR. Mesenchymal stem cell therapy for treatment of craniofacial bone defects: 10 years of experience. Reg Reconst Restor 2016; 1: 1-7
- 10 Mafi R, Hindocha S, Mafi P, Griffin M, Khan WS. Sources of adult mesenchymal stem cells applicable for musculoskeletal applications - a systematic review of the literature. Open Orthop J 2011; 5 (Suppl. 02) 242-248
- 11 Hass R, Kasper C, Böhm S, Jacobs R. Different populations and sources of human mesenchymal stem cells (MSC): a comparison of adult and neonatal tissue-derived MSC. Cell Commun Signal 2011; 9: 12
- 12 Motamedian SR, Tabatabaei FS, Akhlaghi F, Torshabi M, Gholamin P, Khojasteh A. Response of dental pulp stem cells to synthetic, allograft, and xenograft bone scaffolds. Int J Periodont Restor Dent 2017; 37 (01) 49-59
- 13 Gay IC, Chen S, MacDougall M. Isolation and characterization of multipotent human periodontal ligament stem cells. Orthod Craniofac Res 2007; 10 (03) 149-160
- 14 Khojasteh A, Motamedian SR, Rad MR, Shahriari MH, Nadjmi N. Polymeric vs hydroxyapatite-based scaffolds on dental pulp stem cell proliferation and differentiation. World J Stem Cells 2015; 7 (10) 1215-1221
- 15 Miura M, Gronthos S, Zhao M. et al. SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci U S A 2003; 100 (10) 5807-5812
- 16 Motamedian SR, Iranparvar P, Nahvi G, Khojasteh A. Bone tissue engineering: a literature review. Regen Reconst Restor 2016; 1: 103-120
- 17 Motamedian SR, Hosseinpour S, Ahsaie MG, Khojasteh A. Smart scaffolds in bone tissue engineering: a systematic review of literature. World J Stem Cells 2015; 7 (03) 657-668
- 18 Jafari M, Paknejad Z, Rad MR. et al. Polymeric scaffolds in tissue engineering: a literature review. J Biomed Mater Res B Appl Biomater 2017; 105 (02) 431-459
- 19 Hosseinpour S, Ghazizadeh Ahsaie M, Rezai Rad M, Baghani MT, Motamedian SR, Khojasteh A. Application of selected scaffolds for bone tissue engineering: a systematic review. Oral Maxillofac Surg 2017; 21 (02) 109-129
- 20 Safari S, Mahdian A, Motamedian SR. Applications of stem cells in orthodontics and dentofacial orthopedics: current trends and future perspectives. World J Stem Cells 2018; 10 (06) 66-77
- 21 Rody Jr WJ, King GJ, Gu G. Osteoclast recruitment to sites of compression in orthodontic tooth movement. Am J Orthod Dentofacial Orthop 2001; 120 (05) 477-489
- 22 Miyamoto T, Suda T. Differentiation and function of osteoclasts. Keio J Med 2003; 52 (01) 1-7
- 23 Tang L, Li N, Xie H, Jin Y. Characterization of mesenchymal stem cells from human normal and hyperplastic gingiva. J Cell Physiol 2011; 226 (03) 832-842
- 24 Venkatesh D, Kumar KPM, Alur JB. Gingival mesenchymal stem cells. J Oral Maxillofac Pathol 2017; 21 (02) 296-298
- 25 Haque N, Rahman MT, Abu Kasim NH, Alabsi AM. Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy. ScientificWorldJournal 2013; 2013 (2013) 632972
- 26 Nugraha AP, Kitaura H, Ohori F. et al. C–X–C receptor 7 agonist acts as a C–X–C motif chemokine ligand 12 inhibitor to ameliorate osteoclastogenesis and bone resorption. Mol Med Rep 2022; 25 (03) 78
- 27 Shields AM, Panayi GS, Corrigall VM. Resolution-associated molecular patterns (RAMP): RAMParts defending immunological homeostasis?. Clin Exp Immunol 2011; 165 (03) 292-300
- 28 Nugraha AP, Narmada IB, Sitasari PI, Inayati F, Wira R, Triwardhani A. High mobility group box 1 and heat shock protein-70 expression post (-)-epigallocatechin-3-gallate in East Java green tea methanolic extract administration during orthodontic tooth movement in Wistar rats. Pesqui Bras Odontopediatria Clin Integr 2020; 20 (e5347): 1-10
- 29 Nugraha AP, Rantam FA, Narmada IB, Ernawati DS, Ihsan IS. Gingival-derived mesenchymal stem cell from rabbit (Oryctolagus cuniculus): isolation, culture, and characterization. Eur J Dent 2021; 15 (02) 332-339
- 30 Nugraha AP, Prasetyo EP, Kuntjoro M. et al. The effect of cobalt (II) chloride in the viability percentage and the induced hypoxia inducible factor -1α of human adipose mesenchymal stem cells (HAMSCs): an in vitro study. Sys Rev Pharm. 2020; 11 (06) 308-314
- 31 Liu Q, Li M, Wang S, Xiao Z, Xiong Y, Wang G. Recent advances of Osterix transcription factor in osteoblast differentiation and bone formation. Front Cell Dev Biol 2020; 8: 601224
- 32 Kaback LA, Soung Y, Naik A. et al. Osterix/Sp7 regulates mesenchymal stem cell mediated endochondral ossification. J Cell Physiol 2008; 214 (01) 173-182
- 33 Jing J, Hinton RJ, Jing Y, Liu Y, Zhou X, Feng JQ. Osterix couples chondrogenesis and osteogenesis in post-natal condylar growth. J Dent Res 2014; 93 (10) 1014-1021
- 34 Zhao Y, Wang C, Li S. et al. Expression of Osterix in mechanical stress-induced osteogenic differentiation of periodontal ligament cells in vitro. Eur J Oral Sci 2008; 116 (03) 199-206
- 35 Chen X, Liu Y, Ding W. et al. Mechanical stretch-induced osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (hJBMMSCs) via inhibition of the NF-κB pathway. Cell Death Dis 2018; 9 (02) 207
- 36 Han J, He H. Expression and function of osteogenic genes runt-related transcription factor 2 and osterix in orthodontic tooth movement in rats. Int J Clin Exp Pathol 2015; 8 (09) 11895-11900
- 37 Sitasari PI, Narmada IB, Hamid T, Triwardhani A, Nugraha AP, Rahmawati D. East Java green tea methanolic extract can enhance RUNX2 and Osterix expression during orthodontic tooth movement in vivo. J Pharm Pharmacogn Res 2020; 8 (04) 290-298
- 38 Foster BL, Ao M, Salmon CR. et al. Osteopontin regulates dentin and alveolar bone development and mineralization. Bone 2018; 107: 196-207
- 39 Toyota A, Shinagawa R, Mano M, Tokioka K, Suda N. Regeneration in experimental alveolar bone defect using human umbilical cord mesenchymal stem cells. Cell Transplant 2021; 30: 963689720975391
- 40 Brito MV, Pérez MAA, Rodríguez FJM. Osteocalcin expression in periodontal ligament when inducing orthodontic forces. Rev Odontol Mex 2013; 17 (03) 150-153
- 41 Park HJ, Baek KH, Lee HL. et al. Hypoxia inducible factor-1α directly induces the expression of receptor activator of nuclear factor-κB ligand in periodontal ligament fibroblasts. Mol Cells 2011; 31 (06) 573-578
- 42 Ng KT, Li JP, Ng KM, Tipoe GL, Leung WK, Fung ML. Expression of hypoxia-inducible factor-1α in human periodontal tissue. J Periodontol 2011; 82 (01) 136-141
- 43 Boyette LB, Creasey OA, Guzik L, Lozito T, Tuan RS. Human bone marrow-derived mesenchymal stem cells display enhanced clonogenicity but impaired differentiation with hypoxic preconditioning. Stem Cells Transl Med 2014; 3 (02) 241-254
- 44 Dimitriou R, Tsiridis E, Giannoudis PV. Current concepts of molecular aspects of bone healing. Injury 2005; 36 (12) 1392-1404
- 45 Gu Q, Gu Y, Shi Q, Yang H. Hypoxia promotes osteogenesis of human placental-derived mesenchymal stem cells. Tohoku J Exp Med 2016; 239 (04) 287-296
- 46 Hung SP, Ho JH, Shih YR, Lo T, Lee OK. Hypoxia promotes proliferation and osteogenic differentiation potentials of human mesenchymal stem cells. J Orthop Res 2012; 30 (02) 260-266
- 47 Huang YC, Yang ZM, Jiang NG. et al. Characterization of MSCs from human placental decidua basalis in hypoxia and serum deprivation. Cell Biol Int 2010; 34 (03) 237-243
- 48 Ding H, Chen S, Yin JH. et al. Continuous hypoxia regulates the osteogenic potential of mesenchymal stem cells in a time-dependent manner. Mol Med Rep 2014; 10 (04) 2184-2190
- 49 Wang Y, Tang Z, Xue R. et al. Differential response to CoCl2-stimulated hypoxia on HIF-1α, VEGF, and MMP-2 expression in ligament cells. Mol Cell Biochem 2012; 360 (1-2): 235-242
- 50 Song ZC, Li S, Dong JC, Sun MJ, Zhang XL, Shu R. Enamel matrix proteins regulate hypoxia-induced cellular biobehavior and osteogenic differentiation in human periodontal ligament cells. Biotech Histochem 2017; 92 (08) 606-618
- 51 Han XL, Meng Y, Kang N, Lv T, Bai D. Expression of osteocalcin during surgically assisted rapid orthodontic tooth movement in beagle dogs. J Oral Maxillofac Surg 2008; 66 (12) 2467-2475
- 52 Hashimoto F, Kobayashi Y, Mataki S, Kobayashi K, Kato Y, Sakai H. Administration of osteocalcin accelerates orthodontic tooth movement induced by a closed coil spring in rats. Eur J Orthod 2001; 23 (05) 535-545
- 53 Truong LH, Kuliwaba JS, Tsangari H, Fazzalari NL. Differential gene expression of bone anabolic factors and trabecular bone architectural changes in the proximal femoral shaft of primary hip osteoarthritis patients. Arthritis Res Ther 2006; 8 (06) R188
- 54 Mitsui N, Suzuki N, Maeno M. et al. Optimal compressive force induces bone formation via increasing bone sialoprotein and prostaglandin E(2) production appropriately. Life Sci 2005; 77 (25) 3168-3182
- 55 Pavlin D, Goldman ES, Gluhak-Heinrich J, Magness M, Zadro R. Orthodontically stressed periodontium of transgenic mouse as a model for studying mechanical response in bone: the effect on the number of osteoblasts. Clin Orthod Res 2000; 3 (02) 55-66
- 56 Gul Amuk N, Kurt G, Karsli E. et al. Effects of mesenchymal stem cell transfer on orthodontically induced root resorption and orthodontic tooth movement during orthodontic arch expansion protocols: an experimental study in rats. Eur J Orthod 2020; 42 (03) 305-316