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Planta Med 1999; 65(5): 417-420
DOI: 10.1055/s-1999-14018
Papers
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

In vivo Metabolism of Aloemannan

A. Yagi1 , J. Nakamori, T. Yamada, H. Iwase, T. Tanaka, Y. Kaneo, J. Qiu2 , S. Orndorff
  • 1Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Japan
  • 2Univera Pharmaceutical Inc., Broomfield, CO, U.S.A.
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Publication History

1998

1999

Publication Date:
04 January 2007 (online)

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Abstract

The metabolism of fluoresceinyl isothiocyanate labeled aloemannan (FITC-AM) was examined by p.o. and i.v. administration in mice at a dose of 120 mg/kg. Analysis of FITC-AM in urine and feces showed that FITC-AM (MW 500 KD) was metabolized into smaller molecules that mainly accumulated in the kidneys. AM was catabolized by the human intestinal microflora to catabolites 1 and 2 with molecular weights of 30 and 10 KD, respectively. Hydrolysis of AM showed hexosamine peaks on HPAE. The findings suggest that the immunomodulation of AM may come from not only neutral polysaccharides but also contaminated hexosamine in AM.

Key words

Aloemannan - Aloe barbadensis - Asphodelaceae - fluoresceinyl isothiocyanate aloemannan - metabolism - catabolites - intestinal microflora