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Planta Med 1999; 65(8): 735-739
DOI: 10.1055/s-1999-14095
Original Paper

Georg Thieme Verlag Stuttgart · New York

Analysis of Labdane-Type Diterpenes from Cistus creticus (subsp. creticus and subsp. eriocephalus), by GC and GC-MS1

Thalia Anastasaki2 , Costas Demetzos2 , Dimitrios Perdetzoglou2 , Maria Gazouli3 , Anargyros Loukis2 , Caterina Harvala2
  • 1 A part of this work was presented as a poster at the 46th Annual Congress on Medicinal Plant Research, Vienna, 31 Aug. - 4 Sept., 1998
  • 2 School of Pharmacy, Department of Pharmacognosy, University of Athens, Greece
  • 3 Department of Bacteriology, Hellenic Pasteur Institute, Athens, Greece
Further Information

Publication History

January 14, 1999

May 16, 1999

Publication Date:
31 December 1999 (online)

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Abstract

The qualitative and quantitative analysis of labdane-type diterpenes of the hexane extracts and of the essential oils of the leaves, fruits and resin “Ladano”, of Cistus creticus subsp. creticus and Cistus creticus subsp. eriocephalus, have been carried out by GC and GC-MS analysis using two capillary chromatographic columns, i.e., HP-5MS and CP-Wax. The methanolic extract of the fruits of C. creticus subsp. creticus was examined and seven labdane diterpenes were isolated and identified by spectroscopic methods. Data on the investigation of labdane diterpenes by GC and GC-MS is limited and most of them have never been analysed by this method. The results obtained by this analysis could be useful for identifying them in crude plant extracts. Manoyl oxides were studied further for the percentage content of their isomers. The hexane extracts of the two subspecies as well as the manoyl oxide isomers isolated from the methanolic extract of the fruits of C. creticus subsp. creticus, were tested for their antimicrobial activity against Gram-positive and Gram-negative bacteria. Global numerical differences of these C. creticus subspecies, based on labdane diterpenes content in the hexane extracts as well as in the essential oils, were established by statistical methods. Phenotypic differences are discussed.

Key words

Cistus creticus subsp. - Cistaceae - labdane diterpenes - manoyl oxide isomers - GC and GC-MS analysis - antimicrobial activity