Suppression of Rat Neutrophil Reactive Oxygen Species Production and Adhesion by the Diterpenoid Lactone Andrographolide

Abstract

The present study was to examine whether andrographolide, a diterpenoid lactone isolated from the anti-inflammatory herbal medicine Andrographis paniculata (Burm. f.) Nees. (Acanthaceae), has the ability to prevent phorbol-12-myristate-13-acetate (PMA)-induced reactive oxygen species (ROS) production, as well as N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced adhesion by rat neutrophils. Results demonstrated that PMA (100 ng/ml) induced rapid accumulation of H₂O₂ and O₂ ^· in neutrophils within 30 minutes. Andrographolide (0.1 to 10 μM) pretreatment (10 min, 37 °C) significantly attenuated the accumulation of these two oxygen radical metabolites. Administration of andrographolide also significantly prevented fMLP-induced neutrophil adhesion. These data suggest that preventing ROS production and neutrophils adhesion may confer andrographolide the ability to be an anti-inflammatory drug.

Abbreviations

PMA:phorbol-12-myristate-13-acetatefMLP:N-formyl-methionyl-leucyl-phenylalanineROS:reactive oxygen species

Key words

Andrographis paniculata - Acanthaceae - andrographolide - neutrophil - phorbol-12-myristate-13-acetate - reactive oxygen species - N-formyl-methionyl-leucyl-phenylalanine - adhesion

References

• 1 Chang  H M,, But  P PH.. Pharmacology and Application of Chinese Material Medica. Vol. 1: Word Scientific, Singapore; 1987: 918-28
• 2 Yao  X J,, Wainberg  M A,, Parniak  M A.. Mechanism of inhibition of HIV-1 infection in vitro by purified extract of Prunella vulgaris. .  Virology. 1992;;  187 56-2
• 3 Puri  A,, Saxena  R,, Saxena  R P,, Saxena KC, Srivastava  V,, Tandon  J S.. Immunostimulant agents from Andrographis paniculata.  J. Natl. Prod.. 1993;;  56 995-9
• 4 Lu  X J,, Zhang  S L,, Wang  Z S.. Analysis of andrographolide compounds. I. Ion pair high performance liquid chromatographic analysis of andrographolide derivatives.  Acta Pharmaceutica Sinica. 1981;;  16 182-9
• 5 Chiou  W F,, Lin  J J,, Chen  C F.. Andrographolide suppresses the expression of inducible nitric oxide synthase in macrophage and restores the vasoconstriction in rat aorta treated with lipopolysaccharide.  Br. J. Pharmacol.. 1998;;  125 327-34
• 6 Albelda  S M,, Smith  C W,, Ward  P A.. Adhesion molecules and inflammatory injury.  FASEB J.. 1994;;  8 504-12
• 7 Williams  F M.. Role of neutrophils in reperfusion injury.  In: Immunopharmacology of Neutrophils,. Hellewell PG, Williams TJ, editors. Academic Press, San Diego, London; 1994: 245-57
• 8 Shen  Y C,, Chen  C F,, Wang  S Y,, Sung  Y J.. Impediment to calcium influx and reactive oxygen production accounts for the inhibition of neutrophil Mac-1 Up-regulation and adhesion by tetrandrine.  Mol. Pharmacol.. 1999;;  55 186-93
• 9 Shen  Y C,, Sung  Y J,, Chen  C F.. Magnolol inhibits Mac-1 (CD11b/CD18)-dependent neutrophil adhesion: relationship with its antioxidant effect.  Eur. J. Pharmacol.. 1998;;  343 79-86
• 10 Vaporciyan  A A,, Jones  M L,, Ward  P A.. Rapid analysis of leukocyte-endothelial adhesion.  J. Immunol. Methods. 1993;;  159 93-100
• 11 Wardlaw  A J,, Walsh  G M.. Neutrophil adhesion receptors. In: Immunopharmacology of Neutrophils. Hellewell PG, Williams TJ, editors. Academic Press, San Diego, London; 1994: 134-57
• 12 Casimir  C M,, Teahan  C G.. The respiratory burst of neutrophils and its deficiency. Immunopharmacology of Neutrophils. Hellewell PG, Williams TJ, editors. Academic Press, San Diego, London; 1994: 27-54
• 13 Albina  J E,, Mills  C D,, Henry  W L,, Caldwell  M D.. Temporal expression of different pathways of l-arginine metabolism in healing wounds.  J. Immunol.. 1990;;  144 3877-80
• 14 Salmon  J E,, Millard  S S,, Brogle  N L,, Kimberl  R P.. Fc gamma receptor IIIb enhances Fc gamma receptor IIb function in an oxidant-dependent and allele-sensitive manner.  J. Clin. Invest.. 1995;;  95 2877-85
• 15 Yan  S R,, Berton  G.. Regulation of Src family tyrosine kinase activities in adherent human neutrophils. Evidence that reactive oxygen intermediates produced by adherent neutrophils increase the activity of the p58c-fgr and p53/561yn tyrosine kinases.  J. Biol. Chem.. 1996;;  271 23464-71
• 16 Springer  T A,, Anderson  D C.. The importance of the Mac-1, LFA-1 glycoprotein family in monocyte and granulocyte adherence, chemotaxis, and migration into inflammatory sites: insights from an experiment of nature.  Ciba. Found. Symp.. 1986;;  118 102-26
• 17 Tonnesen  M G,, Anderson  D C,, Springer  T A,, Knedler  A,, Avdi  N,, Henson  P M.. Adherence of neutrophils to cultured human microvascular endothelial cells. Stimulation by chemotactic peptides and lipid mediators and dependence upon the Mac-1, LFA-1, p150,95 glycoprotein family.  J. Clin. Invest.. 1989;;  83 637-46
• 18 Deng  W L,, Nie  R J,, Liu  J Y.. Pharmacological studies on thirteen kinds of injections from Andrographis paniculata. I. Antipyretic, anti-inflammatory effects and toxicity.  Chin. Pharm. Bull.. 1982;;  10 38-42
• 19 Serrano  C VJ,, Mikhail  E A,, Wang  P,, Noble  B,, Kuppusamy  P,, Zweier  J L.. Superoxide and hydrogen peroxide induce CD18-mediated adhesion in the postischemic heart.  Biochim. Biophys. Acta. 1996;;  1316 191-202
• 20 Fraticelli  A,, Serrano  C VJ,, Bochner  B S,, Capogrossi  M C,, Zweier  J L.. Hydrogen peroxide and superoxide modulate leukocyte adhesion molecule expression and leukocyte endothelial adhesion.  Biochim. Biophys. Acta. 1996;;  1310 251-9