Oxoaporphine Alkaloids and Quinones from Stephania dinklagei and Evaluation of Their Antiprotozoal Activities

Abstract

Bioactivity-guided fractionation of Stephania dinklagei yielded six compounds including, two zwitterionic oxoaporphine alkaloids, N-methylliriodendronine, and 2-O,N-dimethylliriodendronine, two oxoaporphine alkaloids, liriodenine, and dicentrinone, one aporphine alkaloid, corydine, and one anthraquinone, aloe-emodin. Apart from corydine, the isolates have not been reported as constituents of S. dinklagei. N-Methylliriodendronine, and 2-O,N-dimethylliriodendronine are reported for the first time as natural products. All isolated compound were tested for antiprotozoal activity and cytotoxic activities in vitro. N-Methylliriodendronine was the most active against L. donovani amastigotes (IC₅₀ = 36.1 μM). Liriodenine showed the highest activity against Leishmania donovani, and Plasmodium falciparum with IC₅₀ values of 26.16 and 15 μM, respectively. Aloe-emodin was the only compound active (IC₅₀ = 14 μM) against T. b. brucei.

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