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Planta Med 2002; 68(1): 49-54
DOI: 10.1055/s-2002-20049
Original Paper
Natural Product Chemistry
© Georg Thieme Verlag Stuttgart · New York

Natural Products Inhibiting Candida albicans Secreted Aspartic Proteases from Tovomita krukovii

Zhizhen Zhang1 , Hala N. ElSohly1 , Melissa R. Jacob1 , David S. Pasco1, 2 , Larry A. Walker1, 3 , Alice M. Clark1, 2
  • 11 National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences,
    Thad Cochran Research Center, School of Pharmacy, University of Mississippi,
    University, Mississippi, USA
  • 22 Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, Mississippi, USA
  • 33 Department of Pharmacology, School of Pharmacy, University of Mississippi, University, Mississippi, USA
Further Information

Publication History

January 19, 2001

May 13, 2001

Publication Date:
31 January 2002 (online)

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Abstract

Assay-guided fractionation of the ethanol extract of Tovomita krukovii resulted in the identification of four new xanthones (1 - 4) and ten known compounds (5 - 14). The structures of compounds 1 - 14 were determined by spectral data to be 3,5-dihydroxy-4-methoxyxanthone (1), 1,3,5,7-tetrahydroxy-8-isoprenylxanthone (2), 1,3,5-trihydroxy-8-isoprenylxanthone (3), 1,5,7-trihydroxy-8-isoprenylxanthone (4), 1,3,7-trihydroxy-2-isoprenylxanthone (5), 1,5-dihydroxyxanthone (6), 1,6-dihydroxy-5-methoxyxanthone (7), 1,3,5-trihydroxyxanthone (8), 1,3,6-trihydroxy-5-methoxyxanthone (9), 1,6-dihydroxy-3,5-dimethoxyxanthone (10), 1,3,7-trihydroxyxanthone (11), 3-geranyl-2,4,6-trihydroxybenzophenone (12), betulinic acid (13), and 3,4-dihydroxybenzoic acid (14). Compounds 2, 3, 12 and 13 showed inhibitory effects against Candida albicans secreted aspartic proteases (SAP) with IC50 values of 15 μg/ml, 25 μg/ml, 40 μg/ml, and 6.5 μg/ml, respectively, while the other compounds were inactive. In addition, compound 12 showed activity against C. albicans, C. neoformans, S. aureus and methicillin resistant S. aureus (MRS).

Key words

Tovomita krukovii - Guttiferae - xanthones - prenylated benzophenone - betulinic acid - SAP inhibitors - antimicrobial activity

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Dr. Hala N. ElSohly

National Center for Natural Products Research

School of Pharmacy

University of Mississippi

University

MS 38677

USA

Email: helsohly@olemiss.edu

Fax: +1 662-915-7989