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Planta Med 2002; 68(2): 158-161
DOI: 10.1055/s-2002-20241
Original Paper
Analysis
© Georg Thieme Verlag Stuttgart · New York

Determination of Ginsenosides from Panax ginseng using Micellar Electrokinetic Chromatography

Ingmar Glöckl1, 3 , Markus Veit2 , Gottfried Blaschke3
  • 1Forschungsvereinigung der Arzneimittel-Hersteller e.V., Sinzig, Germany
  • 2Zentralinstitut Arzneimittelforschung GmbH, Sinzig, Germany
  • 3Institute of Pharmaceutical Chemistry, University of Münster, Münster, Germany
Further Information

Publication History

February 2, 2001

April 28, 2001

Publication Date:
22 February 2002 (online)

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Abstract

A method for determination of ginsenosides in herbal medicinal products (HMPs) was developed using micellar electrokinetic chromatography (MEKC). Within 22 minutes 7 major ginsenosides were well separated. In order to demonstrate the accuracy, precision and robustness for the main target analyte the method was exemplarily validated for the determination of Rb1 according to ICH guidelines. Compared to chromatographic analysis, several benefits of capillary electrophoresis (CE) could be demonstrated such as high separation efficiency in an aqueous buffer without any organic solvent and shorter run time per assay.

Key words

Capillary electrophoresis - micellar electrokinetic chromatography - Panax ginseng - Araliaceae - ginsenosides - validation

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Prof. Dr. G. Blaschke

Institute of Pharmaceutical Chemistry

University of Münster

Hittorfstr. 58-62


48149 Münster

Germany

Email: blaschg@uni-muenster.de

Fax: +49-251-83-32144