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Planta Med 2003; 69(5): 483-486
DOI: 10.1055/s-2003-39697
Letter
© Georg Thieme Verlag Stuttgart · New York

Separation and Determination of Coumarins in the Root Bark of Three Citrus Plants by Micellar Electrokinetic Capillary Chromatography

Shu Fang Wang2 , Yong Ju1, 3 , Xing Guo Chen2 , Zhi De Hu2
  • 1National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, P. R. China
  • 2Department of Chemistry, Lanzhou University, Lanzhou, P. R. China
  • 3The Key Laboratory of Bioorganic Phosphorus Chemistry, Ministry of Education, Department of Chemistry, School of Life Science and Engineering, Tsinghua University, Beijing, P. R. China
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Publication History

Received: August 22, 2002

Accepted: December 7, 2002

Publication Date:
12 June 2003 (online)

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Abstract

A micellar electrokinetic capillary chromatography (MEKC) method for the determination of xanthyletin and xanthoxyletin in the roots bark of three Citrus plants was developed for the first time. The electrophoresis buffer was 10 mmol/L sodium borate containing 50 mmol/L sodium dodecyl sulfate, 20 % (v/v) acetonitrile and 10 % (v/v) methanol (pH 9.8). The correlation coefficients of the calibration curves for xanthyletin and xanthoxyletin are 0.9993 and 0.9999, respectively, over the concentration ranges (16.0 - 800.0 mg/L; 8.0 - 400.0 mg/L).

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Prof. Xingguo Chen

Department of Chemistry

Lanzhou University

Lanzhou, 730000

P. R. China

Email: chenxg@lzu.edu.cn

Fax: +86-931-8912582