Polyacetylenic Compounds and Butanol Fraction from Bidens pilosa can Modulate the Differentiation of Helper T Cells and Prevent Autoimmune Diabetes in Non-Obese Diabetic Mice

Abstract

Compelling evidence suggests that infiltrating CD4⁺ type I helper T (Th1) cells in the pancreatic islets play a pivotal role in the progression of diabetes in non-obese diabetic (NOD) mice. We demonstrate in the present report that a butanol fraction of B. pilosa suppressed the development of diabetes, helped maintain levels of blood sugar and insulin in NOD mice in a dose-dependent manner and elevated the serum IgE levels regulated by Th2 cytokines in NOD mice. Moreover, the butanol fraction inhibited the differentiation of naïve helper T (Th0) cells into Th1 cells but enhanced their transition into type II helper T (Th2) cells using an in vitro T cell differentiation assay. Two polyacetylenic compounds, 2-β-D-glucopyranosyloxy-1-hydroxy-5(E)-tridecene-7,9,11-triyne and 3-β-D-glucopyranosyloxy-1-hydroxy-6(E)-tetradecene-8,10,12-triyne, identified from the butanol fraction also prevented the onset of diabetes like the butanol fraction. The latter compound showed a stronger activity for T cell differentiation than the former. In summary, the butanol fraction of B. pilosa and its polyacetylenes can prevent diabetes plausibly via suppressing the differentiation of Th0 cells into Th1 cells and promoting that of Th0 cells into Th2 cells.

Abbreviations

NOD:non-obese diabetic

IDDM:insulin-dependent diabetes mellitus

PBS:phosphate-buffered saline

PMA:phorbol 12-myristate 13-acetate

PHA:phytohemagglutinin from Phaseolus vulgaris

IFN:interferon

IL:interleukin

LT:lymphotoxin

TNF:tumor necrosis factor

HPLC:high performance liquid chromatography

UV:ultraviolet

FACS:fluorescence activated cell sorter

Th:helper T cells

Th0:naïve Th

Th1:type I Th

Th2:type II Th

i. p.:intraperitoneal

MTT:methylthiazoletetrazolium

IC₅₀:inhibitory concentration 50 %

CC₅₀:cytotoxic concentration 50 %

Key words

T helper cells - Differentiation - non-obese diabetes - autoimmune diseases - Bidens pilosa - Asteraceae

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