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Planta Med 2005; 71(1): 77-79
DOI: 10.1055/s-2005-837755
Letter
© Georg Thieme Verlag KG Stuttgart · New York

DNA Topoisomerase I Inhibitor, Ergosterol Peroxide from Penicillium oxalicum

Li-Ming Yang Kuo1 , Kuei-Yuh Chen4 , Shy-Yuan Hwang2 , Jin-Liang Chen3 , Yen-Yin Liu4 , Chia-Ching Liaw1 , Po-Ho Ye5 , Cheng-Jen Chou1 , Chien-Chang Shen1 , Yao-Haur Kuo1
  • 1National Research Institute of Chinese Medicine, Taipei, Taiwan, R.O.C.
  • 2Department of Agriculture and Forestry, Taiwan Endemic Species Research Institute, Nantou County, Taiwan, R.O.C.,
  • 3Department of Hospital and Health Care Administration, Chia Nan University of Pharmacy and Science, Tainan, Taiwan, R.O.C.
  • 4Department of Biology, Chinese Culture University, Taipei, Taiwan, R.O.C.
  • 5Institute of Pharmacology, National Yang Ming University, Taipei, Taiwan, R.O.C.
Further Information

Publication History

Received: May 4, 2004

Accepted: October 8, 2004

Publication Date:
27 January 2005 (online)

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Abstract

Recently, we found that the MeOH extract of Penicillium oxalicum showed inhibitory activity towards DNA topoisomerase I. Subsequently, ergosterol peroxide, ergosterol, palmitoleic acid, and linoleic acid were isolated from the cultured mycelia of P. oxalicum. The structural determinations were based on physical and spectral analyses. Biological evaluation revealed that ergosterol peroxide inhibited the relaxation of supercoiled DNA (pBR322) induced by DNA topoisomerase I, and also showed marginal, selective cytotoxic activity against human colon tumor cells [COLO-205 (ED50) = 8.56 μg/mL].

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Prof. Yao-Haur Kuo

National Research Institute of Chinese Medicine

Shih-Pai

Taipei 112

Taiwan

Republic of China

Phone: +886-2-28201999 ext. 7061

Fax: +886-2-28236150

Email: kuoyh@nricm.edu.tw