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Horm Metab Res 2005; 37(5): 270-274
DOI: 10.1055/s-2005-861468
Original Basic
© Georg Thieme Verlag KG Stuttgart · New York

Possible Involvement of Phosphatidylinositol 3-Kinase/Akt Pathway in Insulin-like Growth Factor-I-induced Alkaline Phosphatase Activity in Osteoblasts

T.  Noda1 , H.  Tokuda1, 2 , M.  Yoshida1 , E.  Yasuda1 , Y.  Hanai2 , S.  Takai1 , O.  Kozawa1
  • 1 Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Japan
  • 2 Department of Clinical Laboratory, National Hospital for Geriatric Medicine, National Center for Geriatrics and Gerontology, Obu, Aichi, Japan
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Publication History

Received 3 November 2004

Accepted after revision 10 March 2005

Publication Date:
22 June 2005 (online)

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Abstract

In the present study, we investigated whether Akt is involved in insulin-like growth factor-I (IGF-I)-stimulated activity of alkaline phosphatase, a marker of mature osteoblast phenotype, in osteoblast-like MC3T3-E1 cells. IGF-I induced the phosphorylation of Akt in these cells. Akt inhibitor significantly suppressed the IGF-I-stimulated alkaline phosphatase activity. The phosphorylation of Akt induced by IGF-I was reduced by the Akt inhibitor. LY294002 and wortmannin, inhibitors of phosphatidylinositol 3-kinase, significantly suppressed the IGF-I-induced alkaline phosphatase activity. The phosphorylation of Akt induced by IGF-I was markedly reduced by LY294002 and wortmannin. These results strongly suggest that phosphatidylinositol 3-kinase/Akt plays a role in the IGF-I-stimulated alkaline phosphatase activity in osteoblasts.

Key words

IGF-I - Alkaline phosphatase - Akt - Osteoblast

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Dr. Osamu Kozawa

Department of Pharmacology, Gifu University Graduate School of Medicine ·

Gifu 501-1194 · Japan

Phone: + 81 (58) 230 62 14

Fax: + 81 (58) 230 62 15

Email: okozawa@cc.gifu-u.ac.jp