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DOI: 10.1055/s-2005-864183
Phospholipase Cγ1 Inhibitory Activities of Prenylated Flavonoids Isolated from Erythrina senegalensis
Publication History
Received: October 2, 2004
Accepted: January 24, 2005
Publication Date:
20 July 2005 (online)
Abstract
Prenylated flavonoids isolated from Erythrina senegalensis were evaluated for their ability to inhibit PLCγ1 activity in vitro and the formation of inositol phosphates (IPt) in PLCγ1-overexpressing NIH3T3 fibroblast cells (NIH3T3γ1 cells). These flavonoids inhibited PLCγ1 activity in a dose-dependent manner (IC50 values ranged from 1.0 μg/mL to 35.0 μg/mL) with the exception of alpinumisoflavone (5). Also erysenegalensein D (2) and erysenegalensein N (3) showed inhibition of inositol phosphate formation in NIH3T3γ1 cells with IC50 values of 13.0 μg/mL and 10 μg/mL, respectively. Analyzing the relation of structure to activity, the number of prenyl groups and the hydroxylation of the prenyl groups were important factors for the inhibition of PLCγ1 activity and of inositol phosphate formation.
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Jong Seog Ahn
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
52 Oeun-dong
Yusong-gu
Daejon 305-333
Korea
Phone: +82-42-860-4312
Fax: +82-42-860-4595
Email: jsahn@kribb.re.kr