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Neuropediatrics 2006; 37(4): 222-228
DOI: 10.1055/s-2006-924724
Original Article

Georg Thieme Verlag KG Stuttgart · New York

Analysis of MxA, IL-4, and IRF-1 Genes in Filipino Patients with Subacute Sclerosing Panencephalitis

J. R. Pipo-Deveza1 , K. Kusuhara2 , C. L. T. Silao3 , M. B. Lukban1 , A. M. Salonga1 , B. C. Sanchez1 , R. Kira2 , M. Takemoto2 , H. Torisu2 , T. Hara2
  • 1Section of Pediatric Neurology, Department of Pediatrics and Neurosciences, University of the Philippines - Philippine General Hospital, Taft Avenue, Manila, Philippines
  • 2Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
  • 3Institute of Human Genetics, National Institutes of Health, University of the Philippines, Taft Avenue, Manila, Philippines
Further Information

Publication History

Received: November 3, 2005

Accepted after Revision: September 18, 2006

Publication Date:
20 December 2006 (online)

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Abstract

Background: Subacute sclerosing panencephalitis (SSPE) is a chronic and debilitating disease of the central nervous system caused by a latent measles virus infection. Three candidate genes, MxA, IL-4, and IRF-1 genes were shown to be associated with SSPE in Japanese patients. These genes have been suggested to play a role in the establishment of persistent viral infection in the central nervous system. Subjects and Methods: Sixty Filipino SSPE patients and 120 healthy control subjects were included in the study. Single nucleotide polymorphisms at promoter regions (IL-4-590C/T and MxA-88G/T) were screened using PCR-RFLP method. Genotyping was done for GT repeat polymorphism within intron 7 of IRF-1. Results: The TT genotype of MxA, as well as the CT genotype of IL-4, were seen a little more frequently among the SSPE patients as compared to the control subjects. The values though, did not reach statistical significance. IRF-1 analysis did not differ between the two groups. Conclusion: Our study failed to demonstrate a significant association between IL-4, MxA, or IRF-1, and SSPE in the Filipino population. Our results might be explained by a greater contribution of environmental factors such as the socio-economic and nutritional factors in the susceptibility of Filipinos to SSPE other than genetic factors.

Key words

Subacute sclerosing panencephalitis (SSPE) - IL-4 - MxA - IRF-1

References

  • 1 Center for Disease Control . SSPE Surveillance - United States.  MMWR. 1982;  43 585-588
    PubMedSearch in Google Scholar
  • 2 Gascon G. Subacute Sclerosing Panencephalitis.  Sem Pediatr Neurology. 1996;  3 260-269
    PubMedSearch in Google Scholar
  • 3 Zilber N, Rannon L, Alter M, Kahana E. Measles, measles vaccination, and risk of subacute sclerosing panencephalitis (SSPE).  Neurology. 1983;  33 1558-1564
    PubMedSearch in Google Scholar
  • 4 Salonga A, Pipo-Deveza J, Lukban M, Sanchez B. The Epidemiology of SSPE at the Philippine General Hospital, 1999 - 2001. For publication. 
  • 5 Sheppard R D, Raine C S, Bornstein M B, Udem S A. Rapid degradation restricts measles virus matrix protein expression in a subacute sclerosing panencephalitis cell line.  Proc Natl Acad Sci USA. 1986;  83 7913-7917
    CrossrefPubMedSearch in Google Scholar
  • 6 Jin L, Beard S, Hunjan R, Brown D WG, Miller E. Characterization of measles virus strains causing SSPE: A study of 11 cases.  Journal of NeuroVirology. 2002;  8 335-344
    PubMedSearch in Google Scholar
  • 7 Kai C, Yamanouchi K, Sakata H, Miyashita N, Takahashi H, Kobune F. Nucleotide sequences of the M gene of prevailing wild measles virus and a comparison with subacute sclerosing panencephalitis virus.  Virus Genes. 1996;  12 85-87
    CrossrefPubMedSearch in Google Scholar
  • 8 Vardas E, Leary P M, Yeats J. Case report and molecular analysis of subacute sclerosing panencephalitis in South African child.  J Clin Microbiol. 1999;  37 775-777
    PubMedSearch in Google Scholar
  • 9 Inoue T, Kira R, Nakao F, Ihara K, Bassuny W MM, Kusuhara K, Nihei K, Takeshita K, Hara T. Contribution of the interleukin 4 gene to susceptibility to subacute sclerosing panencephalitis.  Arch Neurol. 2002;  59 822-827
    CrossrefPubMedSearch in Google Scholar
  • 10 Torisu H, Kusuhara K, Kira R, Bassuny W M, Sakai Y, Hara T. Functional MxA promoter polymorphism associated with subacute sclerosing panencephalitis.  Neurology. 2004;  62 457-460
    CrossrefPubMedSearch in Google Scholar
  • 11 Fujinami R S, Oldstone M B. Alterations in expression of measles virus polypeptides by antibody: molecular events in antibody-induced antigenic modulation.  J Immunol. 1980;  125 78-85
    PubMedSearch in Google Scholar
  • 12 Barett P N, Koschel K, Carter M, ter Meulen V. Effect of measles virus antibodies on a measles SSPE virus persistently infected C6 rat glioma cell line.  J Gen Virol. 1985;  66 1411-1421
    PubMedSearch in Google Scholar
  • 13 Hara T, Yamashita S, Aiba H. et al . Measles virus-specific T helper 1/T helper 2-cytokine production in subacute sclerosing panencephalitis.  Journal of NeuroVirology. 2000;  6 121-126
    CrossrefPubMedSearch in Google Scholar
  • 14 Schneider-Schaulies S, Schneider-Schaulies J, Schuster A, Bayer M, Pavlovic J, ter Meulen V. Cell type-specific MxA-mediated inhibition of measles virus transcription in human brain cells.  J Virol. 1994;  68 6910-6917
    PubMedSearch in Google Scholar
  • 15 Schneider-Schaulies J, Niewiesk S, Meulen V. Measles virus in the CNS: the role of viral and host factors for the establishment and maintenance of a persistent infection.  J Virol. 1999;  5 613-622
    PubMedSearch in Google Scholar
  • 16 Cztonkowska A, Klos M, Iwinska B. Lack of relationship between the HLA system and C4 in subacute sclerosing panencephalitis.  Acta Neurol Scand. 1986;  73 304-305
    PubMedSearch in Google Scholar
  • 17 Harada F, Onisawa S, Suzuki K. HLA and C4 in subacute sclerosing panencephalitis.  Tissue Antigens. 1987;  30 73-75
    PubMedSearch in Google Scholar
  • 18 Aysun S, Ersoy F, Sanal O. HLA Antigen in subacute sclerosing panencephalitis.  Arch Neurology. 1983;  40 38-39
    PubMedSearch in Google Scholar
  • 19 Kusuhara K, Sasaki Y, Nakao F. Analysis of measles virus binding sites of the CD 46 gene in patients with subacute sclerosing panencephalitis.  The Journal of Infectious Diseases. 2000;  181 1447-1449
    CrossrefPubMedSearch in Google Scholar
  • 20 Nester M J. Use of a brief assessment examination in a study of subacute sclerosing panencephalitis.  J Child Neurol. 1996;  11 173-180
    PubMedSearch in Google Scholar
  • 21 DuRant R H, Dyken P R, Swift A V. The influence of inosiplex treatment on the neurological disability of patients with subacute sclerosing panencephalitis.  Journal of Pediatrics. 1982;  101 288-293
    PubMedSearch in Google Scholar
  • 22 Jabbour J T, Duenas D A, Modlin J. SSPE: Clinical staging, course and frequency.  Arch Neurol. 1975;  32 493
    PubMedSearch in Google Scholar
  • 23 Dyken P, Philippart M, Maertens P. The chronic progressive encephalitides of childhood.  Neurological Infections and Epidemiology. 1997;  2 145-158
    PubMedSearch in Google Scholar
  • 24 Jabbour J T, Duena D A, Sever J L. et al . Epidemiology of subacute sclerosing panencephalitis (SSPE) A Report of the SSPE Registry.  JAMA. 1997;  220 961-962
    PubMedSearch in Google Scholar
  • 25 Modlin J F, Jabbour J T, Witte J J, Halsey N A. Epidemiologic studies of measles, measles vaccine, and subacute sclerosing panencephalitis.  Pediatrics. 1977;  54 505-512
    PubMedSearch in Google Scholar
  • 26 The National Vaccine Advisory Committee . The measles epidemic. The problems, barriers, and recommendations.  JAMA. 1991;  266 1547-1552
    CrossrefPubMedSearch in Google Scholar
  • 27 Beersma M FC, Galama J MD, Van Druten H AM. et al . Subacute sclerosing panencephalitis in the Netherlands 1976 - 1990.  Int J of Epidem. 1992;  21 583-588
    PubMedSearch in Google Scholar
  • 28 Okuno Y, Nakao T, Ishida N. et al . Incidence of subacute sclerosing panencephalitis following measles and measles vaccination in Japan.  Int J Epidem. 1989;  18 684-689
    PubMedSearch in Google Scholar
  • 29 Sanders R C, Brian M, Rongap A. et al . High incidence of subacute sclerosing panencephalitis (SSPE) in young children in Papua New Guinea.  Med J Australia. 1990;  153 740
    PubMedSearch in Google Scholar
  • 30 Field Health Service Information System, National Epidemiology Center, Department of Health, Manila. Annual Report. 2003
    Search in Google Scholar

Judy Raful Pipo-Deveza

Section of Pediatric Neurology
Department of Pediatrics and Neurosciences
University of the Philippines - Philippine General Hospital

Taft Avenue

1000 Manila

Philippines

Email: judepipo@yahoo.com