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J Reconstr Microsurg 2006; 22(8): 649-654
DOI: 10.1055/s-2006-956239
Copyright © 2006 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA.

Effects of Intrathecal Administration of FK506 after Sciatic Nerve Crush Injury

Levent Sarikcioglu1 , Erdeniz Duygulu2 , Hulya Aydin3 , Elif Inanc Gurer3 , Olcay Ozkan1 , Serdar Tuzuner4
  • 1Department of Anatomy, Akdeniz University Faculty of Medicine, Antalya
  • 2Department of Orthopaedics and Traumatology, SSK Bursa Sevket Yilmaz Hospital, Bursa
  • 3Department of Neurology, Akdeniz University Faculty of Medicine, Antalya, Turkey
  • 4Department of Orthopaedics and Traumatology, Akdeniz University Faculty of Medicine, Antalya, Turkey
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Publication History

Accepted: June 26, 2006

Publication Date:
30 November 2006 (online)

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ABSTRACT

Although various administration routes of FK506 have been published, intrathecal administration of FK506 has not previously been reported in the literature. A daily dose of 0.05 mg/kg of FK506 was given (a small dose compared with those reported in the available literature). The authors used this small dose to obtain lower immunosuppression and neurotoxicity, and a higher axonal regeneration rate.

A total number of 40 female Wistar rats were used and randomly divided into four groups: control, sham, FK506-treated, and vehicle-treated. Sciatic nerve regeneration was evaluated by walking track analysis, an electrostimulation test, and light microscopic evaluation.

There was a statistically significant difference (p < 0.05) between FK506-treated and vehicle-treated groups at the end of 6 weeks according to both the walking track analysis and the electrostimulation test. Comparing the stimulus thresholds of the sham and FK506-treated group, no significant difference (p > 0,05) was observed.

Evaluation of the data revealed that FK506 had a beneficial effect on sciatic nerve regeneration.

KEYWORDS

FK506 - sciatic nerve crush - recovery - intrathecal administration

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Levent SarikciogluPh.D. 

Department of Anatomy, Akdeniz University Faculty of Medicine

Antalya, Turkey