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Horm Metab Res 1994; 26(2): 92-96
DOI: 10.1055/s-2007-1000780
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© Georg Thieme Verlag, Stuttgart · New York

Adipogenic Activities in Commercial Preparations of Fetuin

M. Bachmeier, G. Löffler
  • Institut für Biochemie, Genetik und Mikrobiologie, Lehrstuhl Biochemie III, Universität Regensburg, Regensburg, Germany
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Publication History

1993

1993

Publication Date:
14 March 2008 (online)

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Summary

Fetuin is frequently in use as a serum substitute especially during the culture of differentiating preadipocyte cell lines. We show here, that the commercially available crude Pedersen fetuin contains a factor permissive for adipose conversion. It can be separated from fetuin by dialysis at acid pH. When 3T3-L1 cells are cultured in the presence of purified Pedersen fetuin, they fail to undergo adipose conversion unless this permissive adipogenic factor is added to the culture medium. Insulin-like growth factor I (IGF-I) and growth hormone (GH) are not able to replace the factor.

Key words

3T3-L1 Predipocytes - Differentiation - Fetuin