Association of NOS3 gene with metabolic syndrome in hypertensive patients

Maria Luisa Fernandez; Rocio Ruiz; Maria Angeles Gonzalez; Reposo Ramirez-Lorca; Carmen Couto; Antonio Ramos; Reyes Gutierrez-Tous; Jose Maria Rivera; Agustin Ruiz; Luis Miguel Real; Antonio Grilo

doi:10.1160/TH04-02-0103

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2004; 92(02): 413-418
DOI: 10.1160/TH04-02-0103

Cell Signalling and Vessel Remodelling

Schattauer GmbH

Association of NOS3 gene with metabolic syndrome in hypertensive patients

Maria Luisa Fernandez

¹Servicio de Medicina Interna, Hospital de Valme, Seville, Spain

,

Rocio Ruiz

²Dpto. de Genómica Estructural, Neocodex, Seville, Spain

,

Maria Angeles Gonzalez

¹Servicio de Medicina Interna, Hospital de Valme, Seville, Spain

,

Reposo Ramirez-Lorca

²Dpto. de Genómica Estructural, Neocodex, Seville, Spain

,

Carmen Couto

³Servicio de Hematología, Hospital de Valme, Seville, Spain

,

Antonio Ramos

¹Servicio de Medicina Interna, Hospital de Valme, Seville, Spain

,

Reyes Gutierrez-Tous

³Servicio de Hematología, Hospital de Valme, Seville, Spain

,

Jose Maria Rivera

¹Servicio de Medicina Interna, Hospital de Valme, Seville, Spain

,

Agustin Ruiz

²Dpto. de Genómica Estructural, Neocodex, Seville, Spain

,

Luis Miguel Real

²Dpto. de Genómica Estructural, Neocodex, Seville, Spain

,

Antonio Grilo

¹Servicio de Medicina Interna, Hospital de Valme, Seville, Spain

› Author Affiliations

Abstract

Summary

Recent data from animal models indicate that the eNOS null mice present a phenotype that resemble the human metabolic syndrome (hypertension, insulin resistance and hypertriglyceridemia). In this work, we have studied whether NOS3 gene, previously related to endothelial dysfunction, might have a role in metabolic syndrome susceptibility in hypertensive patients. To carry out the study, we genotyped 105 hypertensive patients ≤ 60 years old with two polymorphisms of NOS3 gene: 1132 T>C and 7164 G>T (GeneBank:AF519768.1).To check the allelic frequency of these polymorphisms in our geographical area, we also genotyped 94 unselected healthy controls (control group). To perform sample genotyping, we designed a novel FRET system coupled to real time PCR. There were no differences in genotypic distribution or allelic frequency between hypertensive patients and the control group. However, we observed that 786CC genotype was significantly more frequent in hypertensive patients with metabolic syndrome than in those without the syndrome (p=0.0022). When both polymorphisms were analyzed, we identified the 786C894G as the risk haplotype for metabolic syndrome susceptibility (p=0.011). These data suggest a role of the NOS3 gene in the pathogenesis of metabolic syndrome in hypertensive patients.

Keywords

NOS3 - polymorphism - genetics - metabolic syndrome - hypertension

Full Text

References

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