Coagulation factor XIII-A

János Kappelmayer; Ágnes Simon; Éva Katona; Attila Szanto; László Nagy; Attila Kiss; Csongor Kiss; László Muszbek

doi:10.1160/TH05-03-0206

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2005; 94(02): 454-459
DOI: 10.1160/TH05-03-0206

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Coagulation factor XIII-A

A flow cytometric intracellular marker in the classification of acute myeloid leukemias

János Kappelmayer

¹Departments of Clinical Biochemistry and Molecular Pathology, University of Debrecen, Debrecen, Hungary

,

Ágnes Simon

¹Departments of Clinical Biochemistry and Molecular Pathology, University of Debrecen, Debrecen, Hungary

,

Éva Katona

²Departments of Clinical Research Center, University of Debrecen, Debrecen, Hungary

,

Attila Szanto

³Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary

,

László Nagy

³Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary

,

Attila Kiss

⁴2^nd Department of Medicine, University of Debrecen, Debrecen, Hungary

,

Csongor Kiss

⁵Department of Pediatrics, Medical and Health Science Centre, University of Debrecen, Debrecen, Hungary

,

László Muszbek

¹Departments of Clinical Biochemistry and Molecular Pathology, University of Debrecen, Debrecen, Hungary

²Departments of Clinical Research Center, University of Debrecen, Debrecen, Hungary

› Author Affiliations

Abstract

Summary

The association of coagulation factors with leukocytes have been demonstrated in several previous studies. This study was designed to study the sensitivity and specificity of factor XIII subunit A (FXIII-A) labelling in cultured myeloblastic and monoblastic cell lines and to investigate the intracytoplasmic expression of FXIII-A in de novo acute myeloid leukemia (AML) samples. Myeloblastic and a monoblastic cell lines were cultured and investigated for lineage specific maturation markers and FXIII-A expression. Furthermore, FXIII-A expression was investigated in 12 normal samples (7 bone marrow and 5 peripheral blood), 86 de novo AML samples and 6 chronic myelomonocytic leukemia (CMML) samples. In the monoblastic MonoMac6 cell line the appearance of FXIII-A preceded that of CD14 while it remained negative in the myeloblastic PLB-985 cell line throughout its maturation period. Among the AML samples the average frequency of FXIII-A positive cells in myeloblastic leukemia samples was below 10%,while in M4 and M5AML samples it was above 50% and was significantly higher than the generally used CD14 marker (p < 0.0001). In the AML M4 and M5 cases, FXIII-A proved sensitive for the identification of monoblasts.FXIII-A can be considered as a reliable intracytoplasmic marker for the monocytic and megakaryocytic series and its presence is highly predictive for mono- and megakaryocytic AML and for CMML

Keywords

Factor XIII - flow cytometry - acute myeloid leukemia classification

Full Text

References

References
1 Muszbek L, Yee VC, Hevessy Z. Blood coagulation factor XIII: structure and function. Thromb Res 1999; 94: 271-305.
2 Polgár J, Hidasi V, Muszbek L. Non-proteolytic activation of cellular protransglutaminase (placenta macrophage factor XIII). Biochem J 1990; 267: 557-60.
3 Muszbek L, Haramura G, Polgár J. Transformation of cellular factor XIII into an active zymogen transglutaminase in thrombin-stimulated platelets. Thromb Haemost 1995; 74: 702-5.
4 Buluk K. An unknown function of blood platelets. Polski Tygod Lekar 1955; 10: 191-7.
5 Kieselbach TH, Wagner RH. Demonstration of factor XIII in human megakaryocytes by fluorescent antibody technique. Ann NY Acad Sci 1972; 202: 318-28.
6 Muszbek L, Ádány R, Szegedi G. et al. Factor XIII of blood coagulation in human monocytes. Thromb Res 1985; 37: 401-10.
7 Ádány R, Belkin A, Vasilevskaja T. et al. Identification of blood coagulation Factor XIII in human peritoneal macrophages. Eur J Cell Biol 1985; 38: 171-3.
8 Henriksson P, Becker S, Lynch G. et al. Identification of intracellular factor XIII in human monocytes and macrophages. J Clin Invest 1985; 76: 528-34.
9 Ádány R, Kiss A, Muszbek L. Factor XIII: a marker of mono- and megakaryocytopoiesis. Br J Haematol 1987; 67: 167-72.
10 Kappelmayer J, Bacskó G, Kelemen E. et al. Onset and distribution of factor XIII containing cells in the mesenchyme of chorionic villi during early phase of human placentation. Placenta 1994; 15: 613-23.
11 Kappelmayer J, Bacskó G, Birinyi L. et al. Consecutive appearance of coagulation factor XIII subunit A in macrophages, megakaryocytes and liver cells during early phase of human development. Blood 1995; 86: 2191-7.
12 Kreager JA, Devine DV, Greenberg CS. Cytofluorometric identification of plasmin-sensitive factor XIIIa binding to platelets. Thromb Haemost 1988; 60: 88-93.
13 Conkling PR, Achyuthan KE, Greenberg CS. et al. Human mononuclear phagocyte transglutaminase activity cross-links fibrin. Thromb Res 1989; 55: 57-68.
14 Lorand L, Credo RB, Janus TJ. Factor XIII (fibrin stabilizing factor). Methods Enzymol 1981; 80: 333-41.
15 Schwartz ML, Pizzo SV, Hill RL. et al. Human factor XIII from plasma and platelets. J Biol Chem 1973; 248: 1395-407.
16 McDonagh J, Waggoner WG, Hamilton EG. et al. Affinity chromatography of human plasma and platelet factor XIII on organomercurial agarose. Biochim Biophyis Acta 1976; 446: 345-57.
17 Bohn H, Schwick HG. Isolierung und Charakterisierung eines fibrinstabilissierenden Faktor aus menschlichen Plazenten. Arz Forschung 1971; 21: 1432-9.
18 Katona É, Haramura G, Kárpáti L. et al. A simple quick one-step ELISA assay for the determination of complex plasma factor XIII (A₂B₂). Thromb Haemost 2000; 83: 268-73.
19 Katona É, Ajzner É, Tóth K. et al. Enzyme linked immunosorbent assay (ELISA) for the determination of blood coagulation factor XIII A-subunit in plasma and in cell lysates. J Immunol Methods 2001; 258: 127-35.
20 Stahli C, Staehelin T, Miggiano V. et al. High frequencies of antigen specific hybridomas: Dependence on immunisation parameters and prediction by spleen cell analysis. J Immunol Methods 1980; 32: 297-309.
21 Köhler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975; 256: 495-506.
22 Lacombe F, Durrieu F, Briais A. et al. Flow cytometry CD45 gating for immunophenotyping of acute myeloid leukemia. Leukemia 1997; 11: 1878-86.
23 Kappelmayer J, Gratama JW, Karászi É. et al. Flow cytometric detection of intracellular myeloperoxidase, CD3 and CD79a. Interaction between monoclonal antibody clones, fluorochromes and sample preparation protocols J Immunol Methods 2000; 242: 53-65.
24 Kradin RL, Lynch GW, Kurnick JT. et al. Factor XIII A is sythesized and expressed on the surface of U937 cells and alveolar macrophages. Blood 1987; 69: 778-85.
25 Strobl H, Scheinecker C, Csmarits B. et al. Flow cytometric analysis of intracellular CD68 molecule expression in normal and malignant haemopoiesis. Br J Haematol 1995; 90: 774-82.
26 Invernizzi R, De Fazio P, Iannone AM. et al. Immunocytochemical detection of factor XIII A-subunit in acute leukemia. Leuk Res 1992; 16: 829-36.
27 Krasinskas AM, Wasik MA, Kamoun M. at al. The usefuleness of CD64, other monocyte-associated antigens and CD45 gating in the subclassification of acute myeloid leukemias with monocytic differentiation. Am J Clin Pathol 1998; 110: 797-805.
28 Kappelmayer J, Kiss A, Karászi É. et al. Identfication of P-selectin glycoprotein ligand-1 as a useful marker in acute myeloid leukaemias. Br J Haematol 2001; 115: 903-9.
29 Adany R, Nemes Z, Muszbek L. Characterization of factor XIII containing – macrophages in lymph nodes with Hodgkin’s disease. Br J Cancer 1987; 55: 421-6.
30 Nemes Z, Thomazy V, Adany R. et al. Identification of histiocytic reticulum cells by the immunohistochemical demonstration of factor XIII (FXIII-a) in human lymph nodes. J Pathol 1986; 149: 121-32.
31 Toida M, Okumura Y, Swe Win KK. et al. Characterization of cells containing factor XIII subunit a in benign and malignant buccal lesions. Histochem J 1995; 27: 449-56.