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Thromb Haemost 2005; 94(06): 1230-1235
DOI: 10.1160/TH05-05-0344
Platelets and Blood Cells
Schattauer GmbH

Neutrophil P-selectin-glycoprotein-ligand-1 binding to platelet P-selectin enhances metalloproteinase 2 secretion and platelet-neutrophil aggregation

Haissam Abou-Saleh
1   Research Centre, Montreal Heart Institute and University of Montreal, Montreal, Quebec, Canada
,
Jean-François Théorêt
1   Research Centre, Montreal Heart Institute and University of Montreal, Montreal, Quebec, Canada
,
Daniel Yacoub
1   Research Centre, Montreal Heart Institute and University of Montreal, Montreal, Quebec, Canada
,
Yahye Merhi
1   Research Centre, Montreal Heart Institute and University of Montreal, Montreal, Quebec, Canada
› InstitutsangabenFinancial support: Supported by the Canadian Institutes of Health Research (CIHR) and the Heart and Stroke Foundation of Canada (HSFC).
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Publikationsverlauf

Received 18. Mai 2005

Accepted after resubmission 07. Oktober 2005

Publikationsdatum:
07. Dezember 2017 (online)

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Summary

Platelets and neutrophils constitute a high source of metalloproteinases (MMPs), and their interactions via P-selectin and Pselectin- glycoprotein-ligand-1 (PSGL-1) are involved in thrombosis, vascular remodelling, and restenosis. We investigated the impact of these interactions on platelet MMP-2 secretion and function in platelet and neutrophil aggregation. The secretion of MMP-2 from human platelets was significantly increased threefold after thrombin activation, and enhanced two-fold in the presence of neutrophils. Neutrophil supernatant had no effect on platelet MMP-2 secretion. While no MMP-2 was detected in the supernatant of neutrophils, a high amount of MMP-9 was released by neutrophils, and remained unchanged upon thrombin activation or in the presence of platelets. Platelet P-selectin, which increased significantly after activation, triggered platelet binding to neutrophils that was completely inhibited by P-selectin or PSGL-1 antagonists, and was reduced by 50% with a GPIIb/ IIIa antagonist. P-selectin or PSGL-1 antagonism abolished the enhanced secretion of platelet MMP-2 in the presence of neutrophils and reduced platelet-neutrophil aggregation. Platelet activation and binding to neutrophils enhance the secretion of platelet MMP-2 via an adhesive interaction between P-selectin and PSGL-1, which contribute to increase platelet-neutrophil aggregation.

Keywords

Platelets - leukocytes - cell adhesion molecules - metalloproteinases
 

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