Identification of tissue factor and platelet-derived particles on leukocytes during cardiopulmonary bypass by flow cytometry and immunoelectron microscopy

Jihwa Chung; Hidenori Suzuki; Noriyuki Tabuchi; Kengo Sato; Aya Shibamiya; Takatoshi Koyama

doi:10.1160/TH07-01-0025

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2007; 98(02): 368-374
DOI: 10.1160/TH07-01-0025

Platelets and Blood Cells

Schattauer GmbH

Identification of tissue factor and platelet-derived particles on leukocytes during cardiopulmonary bypass by flow cytometry and immunoelectron microscopy

Jihwa Chung

¹Laboratory Molecular Genetics of Hematology, Graduate School of Health Sciences, Tokyo Medical and Dental University, Tokyo, Japan

,

Hidenori Suzuki

²Laboratory for Electron Microscopy, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan

,

Noriyuki Tabuchi

³Division of Thoracic-Cardiovascular Surgery, Yokohama City Minato Red Cross Hospital, Kanagawa, Japan

,

Kengo Sato

¹Laboratory Molecular Genetics of Hematology, Graduate School of Health Sciences, Tokyo Medical and Dental University, Tokyo, Japan

,

Aya Shibamiya

¹Laboratory Molecular Genetics of Hematology, Graduate School of Health Sciences, Tokyo Medical and Dental University, Tokyo, Japan

,

Takatoshi Koyama

¹Laboratory Molecular Genetics of Hematology, Graduate School of Health Sciences, Tokyo Medical and Dental University, Tokyo, Japan

› Institutsangaben

Abstract

Summary

The extrinsic coagulation system initiated by tissue factor (TF) appears to be a major procoagulant stimulus during cardiopulmonary bypass (CPB), although the precise mechanisms remain to be revealed.We recently reported the appearance ofTF-bearing leukocytes during CPB and described their role in promoting coagulation. In this study, we visually identified the in-vivo appearance ofTF-bearing leukocytes and platelet-derived particles on leukocytes in the pericardial blood during cardiac surgery with CPB, by flow cytometry and immunoelectron microscopy. Preliminary flow cytometric experiments showed that the proportion of TF-positive or both TF- and platelet antigen CD41a-positive leukocytes was increased markedly in pericardial blood obtained during CPB,compared with the proportions in preoperative circulating blood. Immunoelectron microscopic analysis revealed that both monocytes and polymorphonuclear leukocytes in the pericardial blood express TF. On the surfaces of these cells, CD41a-positive or both CD41a- and TF-positive platelet-derived particles were observed. Platelet-derived particles include not only microparticles, but also platelets themselves. Leukocytes from preoperative circulating blood contained far fewer of these particles. Our results demonstrate the in-vivo appearance of TF-bearing platelet-derived particles on leukocytes during cardiac surgery with CPB.These findings may be important for the development of strategies to control procoagulant activities during and after cardiac surgery.

Keywords

Tissue factor (TF) - cardiopulmonary bypass (CPB) - monocytes - polymorphonuclear leukocytes (PMNs) - platelet-derived microparticles (MPs) - flow cytometry - immunoelectron microscopy

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Referenzen

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