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Thromb Haemost 2011; 106(02): 344-352
DOI: 10.1160/TH10-12-0810
Platelets and Blood Cells
Schattauer GmbH

Atorvastatin reduces thrombin generation and expression of tissue factor, P-selectin and GPIIIa on platelet-derived microparticles in patients with peripheral arterial occlusive disease

Fariborz Mobarrez
1   Karolinska Institutet Department of Clinical Sciences, Danderyd Hospital, Division of Cardiovascular Medicine, Stockholm, Sweden
,
Shu He
2   Coagulation Research, Department of Molecular Medicine & Surgery, Karolinska Institutet & Clinical Chemistry, Karolinska University Hospital, Solna, Sweden
,
Anders Bröijersen
3   Department of Medicine, Karolinska University Hospital, Huddinge, Sweden
,
Björn Wiklund
4   Department of Vascular Surgery, Karolinska University Hospital, Huddinge, Sweden
,
Aleksandra Antovic
1   Karolinska Institutet Department of Clinical Sciences, Danderyd Hospital, Division of Cardiovascular Medicine, Stockholm, Sweden
,
Jovan Antovic
2   Coagulation Research, Department of Molecular Medicine & Surgery, Karolinska Institutet & Clinical Chemistry, Karolinska University Hospital, Solna, Sweden
,
Nils Egberg
2   Coagulation Research, Department of Molecular Medicine & Surgery, Karolinska Institutet & Clinical Chemistry, Karolinska University Hospital, Solna, Sweden
,
Gun Jörneskog
5   KarolinskaInstitutet, Department of Clinical Sciences, Danderyd Hospital, Division of Internal Medicine, Stockholm, Sweden
,
Håkan Wallén
1   Karolinska Institutet Department of Clinical Sciences, Danderyd Hospital, Division of Cardiovascular Medicine, Stockholm, Sweden
› Author Affiliations
Further Information

Publication History

Received: 21 December 2010

Accepted after major revision: 29 April 2011

Publication Date:
25 November 2017 (online)

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Summary

We investigated the effects of statin treatment on platelet-derived microparticles (PMPs) and thrombin generation in atherothrombotic disease. Nineteen patients with peripheral arterial occlusive disease were randomised to eight weeks of treatment with atorvastatin or placebo in a cross-over fashion. Expression of GPIIIa (CD61), P-selectin (CD62P), tissue factor (TF, CD142) and phosphatidylserine (PS; annexin-V or lactadherin binding) was assessed on PMPs. Thrombin generation in vivo was assessed by measurement of prothrombin fragment 1+2 in plasma (F1+2) and ex vivo by using the calibrated automated thrombogram (CAT). During atorvastatin treatment, expression of TF, P-selectin and GPIIIa was significantly reduced vs. placebo (p<0.001 for all). No effect on annexin-V or lactadherin binding was seen. Thrombin generation was significantly reduced during atorvastatin as assessed by both the CAT assay (p<0.001) and by measurements of F1+2 (p<0.01). Subsequent in vitro experiments showed that when TF on microparticles (MPs) was blocked by antibodies, the initiation of thrombin generation was slightly but significantly delayed. Blocking PS on MPs using annexin-V or lactadherin resulted in almost complete inhibition of thrombin generation. In conclusion, atorvastatin reduces thrombin generation and expression of TF, GPIIIa and P-selectin on PMPs in patients with peripheral vascular disease. Microparticle-bound TF slightly enhances initiation of thrombin generation whereas negatively charged surfaces provided by MPs or lipoproteins could reinforce thrombin generation. Statins may inhibit initiation of thrombin generation partly through a microparticle dependent mechanism but the main effect is probably through reduction of lipoprotein levels.

Keywords

Atorvastatin - peripheral arterial occlusive disease - platelet-derived microparticles - thrombin generation - tissue factor
 

  • References

  • 1 Hooi JD, Kester AD, Stoffers HE. et al. Asymptomatic peripheral arterial occlusive disease predicted cardiovascular morbidity and mortality in a 7-year follow-up study. J Clin Epidemiol 2004; 57: 294-300.
    CrossrefPubMedSearch in Google Scholar
  • 2 Hackam DG, Eikelboom JW. Antithrombotic treatment for peripheral arterial disease. Heart 2007; 93: 303-308.
    CrossrefPubMedSearch in Google Scholar
  • 3 Ludman A, Venugopal V, Yellon DM. et al. Statins and cardioprotection–more than just lipid lowering?. Pharmacol Ther 2009; 122: 30-43.
    CrossrefPubMedSearch in Google Scholar
  • 4 Momsen AH, Jensen MB, Norager CB. et al. Drug therapy for improving walking distance in intermittent claudication: a systematic review and meta-analysis of robust randomised controlled studies. Eur J Vasc Endovasc Surg 2009; 38: 463-474.
    CrossrefPubMedSearch in Google Scholar
  • 5 Ray KK, Cannon CP. The potential relevance of the multiple lipid-independent (pleiotropic) effects of statins in the management of acute coronary syndromes. J Am Coll Cardiol 2005; 46: 1425-1433.
    CrossrefPubMedSearch in Google Scholar
  • 6 George FD. Microparticles in vascular diseases. Thromb Res. 2008; 122 (Suppl. 01) S55-59
    CrossrefPubMedSearch in Google Scholar
  • 7 Boulanger CM. Microparticles, vascular function and hypertension. Curr Opin Nephrol Hypertens. 2010; 19: 177-180.
    CrossrefPubMedSearch in Google Scholar
  • 8 Morel O, Toti F, Hugel B. et al. Procoagulant microparticles: disrupting the vascular homeostasis equation?. Arterioscler Thromb Vasc Biol 2006; 26: 2594-2604.
    CrossrefPubMedSearch in Google Scholar
  • 9 Morel O, Toti F, Hugel B. et al. Cellular microparticles: a disseminated storage pool of bioactive vascular effectors. Curr Opin Hematol 2004; 11: 156-164.
    CrossrefPubMedSearch in Google Scholar
  • 10 Burnier L, Fontana P, Kwak BR. et al. Cell-derived microparticles in haemostasis and vascular medicine. Thromb Haemost 2009; 101: 439-451.
    Thieme ConnectPubMedSearch in Google Scholar
  • 11 Yuana Y, Bertina RM, Osanto S. Pre-analytical and analytical issues in the analysis of blood microparticles. Thromb Haemost 2010; 105: 396-408.
    Thieme ConnectPubMedSearch in Google Scholar
  • 12 Hoffman M, Monroe DM. 3rd. A cell-based model of hemostasis. Thromb Haemost 2001; 85: 958-965.
    Thieme ConnectPubMedSearch in Google Scholar
  • 13 Perneby C, Granström E, Beck O. et al. Optimization of an enzyme immunoassay for 11-dehydro-thromboxane B(2) in urine: comparison with GC-MS. Thromb Res 1999; 96: 427-436.
    CrossrefPubMedSearch in Google Scholar
  • 14 Hemker HC, Al Dieri R, De Smedt E. et al. Thrombin generation, a function test of the haemostatic-thrombotic system. Thromb Haemost 2006; 96: 553-561.
    Thieme ConnectPubMedSearch in Google Scholar
  • 15 Mobarrez F, Antovic J, Egberg N. et al. A multicolor flow cytometric assay for measurement of platelet-derived microparticles. Thromb Res 2010; 125: e110-116.
    CrossrefPubMedSearch in Google Scholar
  • 16 Dasgupta SK, Abdel-Monem H, Niravath P. et al. Lactadherin and clearance of platelet-derived microvesicles. Blood 2009; 113: 1332-1339.
    CrossrefPubMedSearch in Google Scholar
  • 17 Takemoto M, Liao JK. Pleiotropic effects of 3-hydroxy-3-methylglutaryl coenzyme a reductase inhibitors. Arterioscler Thromb Vasc Biol 2001; 21: 1712-1719.
    CrossrefPubMedSearch in Google Scholar
  • 18 Labiós M, Martínez M, Gabriel F. et al. Effect of atorvastatin upon platelet activation in hypercholesterolemia, evaluated by flow cytometry. Thromb Res 2005; 115: 263-270.
    CrossrefPubMedSearch in Google Scholar
  • 19 Malmström RE, Settergren M, Böhm F. et al. No effect of lipid lowering on platelet activity in patients with coronary artery disease and type 2 diabetes or impaired glucose tolerance. Thromb Haemost 2009; 101: 157-164.
    Thieme ConnectPubMedSearch in Google Scholar
  • 20 Lijnen P, Echevaría-Vázquez D, Petrov V. Influence of cholesterol-lowering on plasma membrane lipids and function. Methods Find Exp Clin Pharmacol 1996; 18: 123-136.
    PubMedSearch in Google Scholar
  • 21 Vaughan CJ, Gotto AM, Jr Basson CT. The evolving role of statins in the management of atherosclerosis. J Am Coll Cardiol 2000; 35: 1-10.
    CrossrefPubMedSearch in Google Scholar
  • 22 Bach RR. Tissue factor encryption. Arterioscler Thromb Vasc Biol 2006; 26: 456-461.
    PubMedSearch in Google Scholar
  • 23 Tramontano AF, O’Leary J, Black AD. et al. Statin decreases endothelial microparticle release from human coronary artery endothelial cells: implication for the Rho-kinase pathway. Biochem Biophys Res Commun 2004; 320: 34-38.
    CrossrefPubMedSearch in Google Scholar
  • 24 Sommeijer DW, Joop K, Leyte A. et al. Pravastatin reduces fibrinogen receptor gpIIIa on platelet-derived microparticles in patients with type 2 diabetes. J Thromb Haemost 2005; 3: 1168-1171.
    CrossrefPubMedSearch in Google Scholar
  • 25 Nomura S, Shouzu A, Omoto S. et al. Effects of losartan and simvastatin on monocyte-derived microparticles in hypertensive patients with and without type 2 diabetes mellitus. Clin Appl Thromb Hemost 2004; 10: 133-141.
    CrossrefPubMedSearch in Google Scholar
  • 26 Tehrani S, Mobarrez F, Antovic A. et al. Atorvastatin has antithrombotic effects in patients with type 1 diabetes and dyslipidemia. Thromb Res 2010; 126: e225-31.
    CrossrefPubMedSearch in Google Scholar
  • 27 Lechner D, Weltermann A. Circulating tissue factor-exposing microparticles. Thromb Res 2008; 122: 47-54.
    CrossrefPubMedSearch in Google Scholar
  • 28 Diamant M, Nieuwland R, Pablo RF. et al. Elevated numbers of tissue-factor exposing microparticles correlate with components of the metabolic syndrome in uncomplicated type 2 diabetes mellitus. Circulation 2002; 106: 2442-2447.
    CrossrefPubMedSearch in Google Scholar
  • 29 Rota S, William NA, Baglin TP. et al. Atherogenic lipoproteins support assembly of the prothrombinase complex and thrombin generation: modulation by oxidation and vitamin E. Blood 1999; 91: 508-515.
    PubMedSearch in Google Scholar
  • 30 Bröijersén A, Hamsten A, Silveira A. et al. Gemfibrozil reduces thrombin generation in patients with combined hyperlipidaemia, without influencing plasma fibrinogen, fibrin gel structure or coagulation factor VII. Thromb Haemost 1996; 76: 171-176.
    Thieme ConnectPubMedSearch in Google Scholar
  • 31 Notarbartolo A, Davì G, Averna M. et al. Inhibition of thromboxane biosynthesis and platelet function by simvastatin in type IIa hypercholesterolemia. Arterioscler Thromb Vasc Biol 1995; 15: 247-251.
    CrossrefPubMedSearch in Google Scholar