Selective iNOS-inhibition does not influence apoptosis in ruptured canine cranial cruciate ligaments

D. Hofer; S. Forterre; A. Schweighauser; M. Krayer; M. Doherr; P. Schawalder; A. Zurbriggen; D. Spreng

doi:10.3415/VCOT-08-09-0078

Veterinary and Comparative Orthopaedics and Traumatology, Table of Contents

Vet Comp Orthop Traumatol 2009; 22(03): 198-203
DOI: 10.3415/VCOT-08-09-0078

Original Research

Schattauer GmbH

Selective iNOS-inhibition does not influence apoptosis in ruptured canine cranial cruciate ligaments

D. Hofer

¹Division of Small Animal Surgery and Orthopedics, Vetsuisse Faculty Berne, Department of Clinical Veterinary Medicine, University of Berne, Berne,Switzerland

,

S. Forterre

¹Division of Small Animal Surgery and Orthopedics, Vetsuisse Faculty Berne, Department of Clinical Veterinary Medicine, University of Berne, Berne,Switzerland

,

A. Schweighauser

²Division of Small Animal Medicine, Department of Clinical Veterinary Medicine, University of Berne, Berne,Switzerland

,

M. Krayer

¹Division of Small Animal Surgery and Orthopedics, Vetsuisse Faculty Berne, Department of Clinical Veterinary Medicine, University of Berne, Berne,Switzerland

,

M. Doherr

³Division of Clinical Research, Department of Clinical Veterinary Medicine, University of Berne, Berne,Switzerland

,

P. Schawalder

¹Division of Small Animal Surgery and Orthopedics, Vetsuisse Faculty Berne, Department of Clinical Veterinary Medicine, University of Berne, Berne,Switzerland

,

A. Zurbriggen

³Division of Clinical Research, Department of Clinical Veterinary Medicine, University of Berne, Berne,Switzerland

,

D. Spreng

¹Division of Small Animal Surgery and Orthopedics, Vetsuisse Faculty Berne, Department of Clinical Veterinary Medicine, University of Berne, Berne,Switzerland

› Author Affiliations

Abstract

Summary

Abnormal patterns of cell death, including increased apoptosis, can influence homeostasis of ligaments and could be involved in the pathogenesis of cranial cruciate ligament (CCL) rupture. Increased nitric oxide (NO) production has been implicated as a stimulus to increased apoptosis in articular cartilage. This study investigated apoptotic cell death in ruptured canine CCL (CCL group, n = 15), in ruptured CCL of dogs treated with oral L-N6-(1-iminoethyl)-lysine (L-NIL), a selective NO-synthetase(NOS)-inhibitor, (L-NIL group, n = 15) and compared the results with normal canine CCL (control group, n = 10).

Orally administered L-NIL at a dosage of 25 mg/m²of body surface area was effective in inhibiting NO production in the articular cartilage of dogs in the L-NIL group, but it did not significantly influence the increased quantity of apoptotic cells found in ruptured CCL specimens. The results of this study suggest that apoptosis of ligamentocytes in the canine CCL is not primarily influenced by increased NO production within the stifle joint.

Keywords

Apoptosis - caspase - cranial cruciate ligament - dog - nitric oxide

Full Text

References

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