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CC BY-NC-ND 4.0 · J Lab Physicians 2010; 2(02): 061-065
DOI: 10.4103/0974-2727.72150
Original Article

Emerging Infections: Shewanella – A Series of Five Cases

Krishna Kanchan Sharma
Department of Microbiology, Sri. Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, India
,
Usha Kalawat
Department of Microbiology, Sri. Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, India
› Author Affiliations Source of Support: Nil
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ABSTRACT

Background: Shewanella spp. are unusual cause of disease in humans; however, reports of Shewanella infections have been increasing. Shewanella is a ubiquitous organism that has been isolated from many foods, sewage, and both from fresh and salt water. Earlier it was named as Pseudomonas putrefaciens or Shewanella putrefaciens. There are several reports describing this organism causing human infections such as cellulitis, abscesses, bacteremia, wound infection, etc. It is oxidase and catalase-positive non-fermenter gram-negative rod that produces hydrogen sulfide.

Aims: The study was conducted to identify Shewanella spp., which was wrongly reported as Pseudomonas spp.

Materials and Methods: Clinical samples were cultured as per standard clinical laboratory procedure. We tested the non-lactose-fermenting colonies for oxidase positivity. Oxidase-positive colony was inoculated in triple sugar iron slant (TSI) to know the hydrogen sulfide production. Hydrogen sulfide positive colonies were further tested for citrate, urease, indole, and amino acid decarboxylation and acid and gas production from sugars.

Results: Five isolates identified as Pseudomonas spp. during preliminary testing were proved to be Shewanella spp. on further testing.

Conclusions: It will help in better understanding the epidemiology, pathogenesis and risk factors associated with these and prevention of the rare pathogenic organisms.

Keywords

Rare pathogens - non-healing ulcer - chronic ulcer - Shewanella algae infection - Shewanella putrefaciens - emerging pathogens


Publication History

Article published online:
29 January 2020

© 2010.

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  • REFERENCES

  • 1 Winn W Jr, Allen S, Janda W, Koneman E, Procop G, Schrenberger P, Woods G. Koneman’s colour Atlas and Textbook of Diagnostic Microbiology. 6th ed. Lippincott Williams and Wilkins (USA); 2006. p. 303-91.
  • 2 MacDonnell MT, Colwell RR. Phylogeny of the Vibrionaceae and recommendation for 2 new genera, Listonella and Shewanella. Syst Appl Microbiol 1985;6:171-82.
  • 3 Derby HA, Hammer HA. Bacteriology of butter. IV. Bacteriological studies on surface taint butter. Iowa Agric Exp Station Res Bull 1931;145:389-416.
  • 4 Holt HM, Søgaard P, Gahrn-Hansen B. Ear infections with Shewanella alga. A bacteriologic, clinical and epidemiologic study of 67 cases. Clin Microbiol Infect 1997;3:329-34.
  • 5 Myrug DS, Jung YS, Kang S, Song YA, Park KH, Jung S, et al. Primary Shewanella algae bacterimia mimicking Vibrio septicemia. J Koran Med Sci 2009;24:1192-4.
  • 6 Chen YS, Liu YC, Yen MY, Wang JH, Wang JH, Wann SR, et al. Skin and soft tissue manifestation of Shewanella putrefaciens infection. Clin Infect Dis 1997;25:225-9.
  • 7 Tsai TH, You HY. Necrotizing fasciitis caused by Shewanella putrefaciens in uremic patients. J Microbiol Immunol Infect 2006;39:516-8.
  • 8 NCCLS, Performance Standard for Antimicrobial Disk Susceptibility Tests. Approved Standard. 8th ed. NCCLS Document M2- A8 Wayne, Pa: NCCLS: 2003.
  • 9 Beaver PC, Jung RC, Cupp EW. Clinical Parasitology. 9th ed. Bombay: Indian Edition K M Varghese; 1984. p. 733-64.
  • 10 Ivanova EP, Flavier S, Christen R. Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov. Int J Syst Evol Microbiol 2004;54:1773-88.
  • 11 Gilardi GL. Infrequently encountered Pseudomonas species causing infection in humans. Ann Intern Med 1972;77:211-5.
  • 12 Brink AJ, van Straten A, van Rensburg AJ. Shewanella (Pseudomonas) putrefaciens bacteremia. Clin Infect Dis 1995;20:1327-32.
  • 13 Simudu UK, Kita-Tsukamoto T, Yasumoto, Yotsu M. Taxonomy of four marine bacterial strains that produce tetrodotoxin. Int J Syst Bacteriol 1990;40:331-6.
  • 14 Nozue H, Hayashi T, Hashimoto Y, Ezaki T, Hamaski K, Ohwada K,et al. Isolation and characterization of Shewanella alga from human clinical specimens and emendation of the description of S. alga Simidu et al, 1990, 335. Int J Syst Bacteriol 1992;42:628-34
  • 15 Khashe S, Janda JM. Biochemical and pathogenic properties of Shewanella alga and Shewanella putrefaciens. J Clin Microbiol 1998;36:783-7.
  • 16 Vogel BF, Jorgensen K, Christensen H, Olsen JE, Gram L. Differentiation of Shewanella putrefaciens and Shewanella alga on the basis of whole-cell protein profiles, ribotyping, phenotypic characterization, and 16S rRNA gene sequence analysis. Appl Environ Microbiol 1997;63:2189-99.
  • 17 Papanaoum K, Marshmann G, Gordon LA, Lumb R, Gordon DL. Concurrent infection due to Shewanella putrefaciens and Mycobacterium marinum acquired at the beach. Aust J Dermatol 1998;39:92-5.
  • 18 Chen SC, Lawrence RH, Packham DR, Sorrell TC. Cellulitis due to Pseudomonas putrefaciens: possible production of exotoxins. Rev Infect Dis 1991;13:642-43.
  • 19 Vandepitte J, Debois J. Pseudomonas putrefaciens as a cause of bacteremia in humans. J Clin Microbiol 1978;7:70-72.
  • 20 Bulut C, Ertem GT, Gokcek C, Tulek N, Bayar MA, Karakoc E. A rare cause of wound infection: Shewanella putrefaciens. Scand J Infect Dis 2004;36:692-94.
  • 21 Holt HM, Gahrn-Hansen B, Bruun B. Shewanella species: infections in Denmark and phenotypic characterisation. Clin Microbiol Infect 2004;10:348-9.
  • 22 Kim JH, Cooper RA, Welty WK, Harrell LJ, Zwadyk P, Klotman ME. Pseudomonas putrefaciens bacteremia. Rev Infect Dis 1989;11:97-104.