ABSTRACT
Objectives: This study was carried out to examine the effect of application of four different
disinfecting agents on the micro-shear bond strength (μ-SBS) of an etch-and-rinse
and self-etch adhesive systems. Materials and Methods: One hundred flat dentin surfaces of human molars were produced by wet grinding the
buccal surfaces. Specimens were randomly assigned to five groups according to the
disinfectant used: Group I: Control (no disinfectant); Group II: 5.25% sodium hypochlorite
based; Group III: 2% chlorhexidine based (Consepsis), Group IV: 0.1% benzalkoniumchloride
based (Tubulicid red) and Group V: 3% doxycycline based (Biopure, MTAD). Specimens
were bonded using either Adper Single Bond 2 or Clearfil S3 Bond, which were employed according to the manufacturer's instructions. Resin composite
microcylinders were bonded using Tygon® tubes for μ-SBS testing. The modes of failure were noted after visual examination
using a binocular stereomicroscope at ×25 magnification. Failures were classified
as adhesive, or mixed. μ-SBS results were analyzed using two-way ANOVA followed by
Tukey's post-hoc test. Results: Dentin disinfectants tested significantly negated the bonding of Adper Single bond
2 and the groups were ranked; Group I > Group V = Group IV > Group II = Group III,
meanwhile they enhanced significantly the μ-SBS values upon using Clearfil S3 Bond and were ranked; Group II > Group III = Group IV = Group V > Group I. Most failures
were adhesive with the Adper single bond adhesive system. Mixed modes of failure were
evident with Clearfil S3 bond. Conclusions: The disinfectants tested should not be used with Adper Single Bond 2 when applied
before the etching step, However they could be used safely prior to bonding with Clearfil
S3 Bond.
Key words:
Benzalkonium chloride - chlorhexidine - doxycycline - sodium hypochlorite