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CC BY-NC-ND 4.0 · Eur J Dent 2014; 08(03): 307-313
DOI: 10.4103/1305-7456.137631
Original Article
Dental Investigation Society

Comparison of phenotype and differentiation marker gene expression profiles in human dental pulp and bone marrow mesenchymal stem cells

Deepa Ponnaiyan
1   Department of Periodontics, S.R.M Dental College and Hospital, Ramapuram, Chennai, Tamil Nadu, India
,
Visakan Jegadeesan
2   Department of Oral and Maxillofacial Surgery, M.I.O.T Hospitals, Chennai, Tamil Nadu, India
› Author Affiliations
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Publication History

Publication Date:
25 September 2019 (online)

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ABSTRACT

Objective: Bone marrow (BM) is the most utilized and well-studied source of stem cells. Stem cells from dental tissues have provided an alternate source of mesenchymal stem cells (MSCs). Dental pulp stem cells (DPSCs) have been shown to share a similar pattern of protein expression with BMMSCs in vitro. However, differences have been noted between DPSCs and BMMSCs. This study focuses on variation in expression of stem cell and differentiation markers between DPSCs and BMMSCs. Materials and Methods: The two stem cells were isolated and compared for clonogenic potential, growth characteristics, multipotency, and stem cell marker expression. Specifically, the fatty acid binding protein 4, perilipin, alkaline phosphatase and osteonectic gene expression was analyzed by real-time polymerase chain reaction to confirm the capacity for adipogenic and osteogenic differentiation. Results: MSCs from these cell sources were similar in their morphology and immune phenotype except for the expression of CD105. Growth curves and colony formation assay revealed proliferation rate of DPSCs was significantly faster than BMMSCs (P < 0.05). DPSCs appeared less able to differentiate into adipogenic lineage, although more able to differentiate into osteogenic lineage. Conclusion: Data from the present study indicate how DPSCs are different from BMMSCs though they are a population of MSCs. DPSCs are a novel population of MSCs as observed by their unique expression of differentiation and lineage specific genes. Further microarray analysis could be used to determine, which genes are differentially regulated in BMMSCs and DPSCs to establish uniqueness of each population of MSCs.

Key words:

Bone marrow - comparative analysis - dental pulp - differentiation - mesenchymal stem cells
 

  • REFERENCES

  • 1 Friedenstein AJ, Petrakova KV, Kurolesova AI, Frolova GP. Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation 1968; 6: 230-47
    CrossrefPubMedSearch in Google Scholar
  • 2 Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H. et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 2002; 13: 4279-95
    CrossrefPubMedSearch in Google Scholar
  • 3 Toma JG, Akhavan M, Fernandes KJ, Barnabé-Heider F, Sadikot A, Kaplan DR. et al. Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 2001; 3: 778-84
    CrossrefPubMedSearch in Google Scholar
  • 4 Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo . Proc Natl Acad Sci U S A 2000; 97: 13625-30
    CrossrefPubMedSearch in Google Scholar
  • 5 Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J. et al. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 2004; 364: 149-55
    CrossrefPubMedSearch in Google Scholar
  • 6 Bosch P, Musgrave DS, Lee JY, Cummins J, Shuler T, Ghivizzani TC. et al. Osteoprogenitor cells within skeletal muscle. J Orthop Res 2000; 18: 933-44
    CrossrefPubMedSearch in Google Scholar
  • 7 Rogers I, Casper RF. Umbilical cord blood stem cells. Best Pract Res Clin Obstet Gynaecol 2004; 18: 893-908
    CrossrefPubMedSearch in Google Scholar
  • 8 Yen BL, Huang HI, Chien CC, Jui HY, Ko BS, Yao M. et al. Isolation of multipotent cells from human term placenta. Stem Cells 2005; 23: 3-9
    CrossrefPubMedSearch in Google Scholar
  • 9 Kern S, Eichler H, Stoeve J, Klüter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells 2006; 24: 1294-301
    CrossrefPubMedSearch in Google Scholar
  • 10 Chang YJ, Shih DT, Tseng CP, Hsieh TB, Lee DC, Hwang SM. Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood. Stem Cells 2006; 24: 679-85
    CrossrefPubMedSearch in Google Scholar
  • 11 Jin HJ, Park SK, Oh W, Yang YS, Kim SW, Choi SJ. Down-regulation of CD105 is associated with multi-lineage differentiation in human umbilical cord blood-derived mesenchymal stem cells. Biochem Biophys Res Commun 2009; 381: 676-81
    CrossrefPubMedSearch in Google Scholar
  • 12 Liechty KW, MacKenzie TC, Shaaban AF, Radu A, Moseley AM, Deans R. et al. Human mesenchymal stem cells engraft and demonstrate site-specific differentiation after in utero transplantation in sheep. Nat Med 2000; 6: 1282-6
    CrossrefPubMedSearch in Google Scholar
  • 13 Stenderup K, Justesen J, Clausen C, Kassem M. Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells. Bone 2003; 33: 919-26
    CrossrefPubMedSearch in Google Scholar
  • 14 Fan M, Chen W, Liu W, Du GQ, Jiang SL, Tian WC. et al. The effect of age on the efficacy of human mesenchymal stem cell transplantation after a myocardial infarction. Rejuvenation Res 2010; 13: 429-38
    CrossrefPubMedSearch in Google Scholar
  • 15 Gronthos S, Zannettino AC, Hay SJ, Shi S, Graves SE, Kortesidis A. et al. Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. J Cell Sci 2003; 116: 1827-35
    CrossrefPubMedSearch in Google Scholar
  • 16 Seo BM, Miura M, Sonoyama W, Coppe C, Stanyon R, Shi S. Recovery of stem cells from cryopreserved periodontal ligament. J Dent Res 2005; 84: 907-12
    Search in Google Scholar
  • 17 Chen SC, Marino V, Gronthos S, Bartold PM. Location of putative stem cells in human periodontal ligament. J Periodontal Res 2006; 41: 547-53
    CrossrefPubMedSearch in Google Scholar
  • 18 Sonoyama W, Liu Y, Yamaza T, Tuan RS, Wang S, Shi S. et al. Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: A pilot study. J Endod 2008; 34: 166-71
    CrossrefPubMedSearch in Google Scholar
  • 19 Shi S, Robey PG, Gronthos S. Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis. Bone 2001; 29: 532-9
    CrossrefPubMedSearch in Google Scholar
  • 20 Yamada Y, Fujimoto A, Ito A, Yoshimi R, Ueda M. Cluster analysis and gene expression profiles: A cDNA microarray system-based comparison between human dental pulp stem cells (hDPSCs) and human mesenchymal stem cells (hMSCs) for tissue engineering cell therapy. Biomaterials 2006; 27: 3766-81
    CrossrefPubMedSearch in Google Scholar
  • 21 Pierdomenico L, Bonsi L, Calvitti M, Rondelli D, Arpinati M, Chirumbolo G. et al. Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp. Transplantation 2005; 80: 836-42
    CrossrefPubMedSearch in Google Scholar
  • 22 Prockop DJ, Sekiya I, Colter DC. Isolation and characterization of rapidly self-renewing stem cells from cultures of human marrow stromal cells. Cytotherapy 2001; 3: 393-6
    CrossrefPubMedSearch in Google Scholar
  • 23 Lin NH, Gronthos S, Bartold PM. Stem cells and future periodontal regeneration. Periodontol 2000 2009; 51: 239-51
    CrossrefPubMedSearch in Google Scholar
  • 24 Janeway Jr CA, Travers P. Antigen presentation to T lymphocytes. In: Janeway CA, Travers P, Walport M, Shlomchik M. editors. Immunobiology. 6th ed.. New York: Garland Science; 2005: p. 70-7
    Search in Google Scholar
  • 25 Ninomiya Y, Sugahara-Yamashita Y, Nakachi Y, Tokuzawa Y, Okazaki Y, Nishiyama M. Development of a rapid culture method to induce adipocyte differentiation of human bone marrow-derived mesenchymal stem cells. Biochem Biophys Res Commun 2010; 394: 303-8
    CrossrefPubMedSearch in Google Scholar
  • 26 Huang GT, Yamaza T, Shea LD, Djouad F, Kuhn NZ, Tuan RS. et al. Stem/progenitor cell-mediated de novo regeneration of dental pulp with newly deposited continuous layer of dentin in an in vivo model. Tissue Eng Part A 2010; 16: 605-15
    CrossrefPubMedSearch in Google Scholar
  • 27 Odabaş S, Sayar F, Güven G, Yanikkaya-Demirel G, Pişkin E. Separation of mesenchymal stem cells with magnetic nanosorbents carrying CD105 and CD73 antibodies in flow-through and batch systems. J Chromatogr B Analyt Technol Biomed Life Sci 2008; 861: 74-80
    CrossrefPubMedSearch in Google Scholar
  • 28 Conley BA, Koleva R, Smith JD, Kacer D, Zhang D, Bernabéu C. et al. Endoglin controls cell migration and composition of focal adhesions: Function of the cytosolic domain. J Biol Chem 2004; 279: 27440-9
    CrossrefPubMedSearch in Google Scholar
  • 29 Siggins RW, Zhang P, Welsh D, Lecapitaine NJ, Nelson S. Stem cells, phenotypic inversion, and differentiation. Int J Clin Exp Med 2008; 1: 2-21
    PubMedSearch in Google Scholar
  • 30 Ponnaiyan D, Bhat KM, Bhat GS. Comparison of immuno-phenotypes of stem cells from human dental pulp and periodontal ligament. Int J Immunopathol Pharmacol 2012; 25: 127-34
    CrossrefPubMedSearch in Google Scholar
  • 31 Karahuseyinoglu S, Cinar O, Kilic E, Kara F, Akay GG, Demiralp DO. et al. Biology of stem cells in human umbilical cord stroma: In situ and in vitro surveys. Stem Cells 2007; 25: 319-31
    CrossrefPubMedSearch in Google Scholar
  • 32 Moerman EJ, Teng K, Lipschitz DA, Lecka-Czernik B. Aging activates adipogenic and suppresses osteogenic programs in mesenchymal marrow stroma/stem cells: The role of PPAR-gamma2 transcription factor and TGF-beta/BMP signaling pathways. Aging Cell 2004; 3: 379-89
    CrossrefPubMedSearch in Google Scholar
  • 33 Stein GS, Lian JB. Molecular mechanisms mediating proliferation/differentiation interrelationships during progressive development of the osteoblast phenotype. Endocr Rev 1993; 14: 424-42
    CrossrefPubMedSearch in Google Scholar
  • 34 Kruger E, Thomson WM, Konthasinghe P. Third molar outcomes from age 18 to 26: Findings from a population-based New Zealand longitudinal study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001; 92: 150-5
    CrossrefPubMedSearch in Google Scholar
  • 35 Eslaminejad MB, Vahabi S, Shariati M, Nazarian H. In vitro Growth and characterization of stem cells from human dental pulp of deciduous versus permanent teeth. J Dent (Tehran) 2010; 7: 185-95
    Search in Google Scholar