Comparative evaluation of photodynamic therapy using LASER or light emitting diode on cariogenic bacteria: An in vitro study

Liliana Guimarães Oliveira Ricatto; Luis Augusto Lopato Conrado; Cecilia Pedroso Turssi; Fabiana Mantovani Gomes França; Roberta Tarkany Basting; Flávia Lucisano Botelho Amaral

doi:10.4103/1305-7456.143634

European Journal of Dentistry, Table of Contents

CC BY-NC-ND 4.0 · Eur J Dent 2014; 08(04): 509-514
DOI: 10.4103/1305-7456.143634

Original Article

Dental Investigation Society

Comparative evaluation of photodynamic therapy using LASER or light emitting diode on cariogenic bacteria: An in vitro study

Liliana Guimarães Oliveira Ricatto

¹Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil

,

Luis Augusto Lopato Conrado

²Department of Biomedical Engineering, Unicastelo, São José dos Campos, São Paulo, Brazil

,

Cecilia Pedroso Turssi

¹Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil

,

Fabiana Mantovani Gomes França

¹Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil

,

Roberta Tarkany Basting

¹Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil

,

Flávia Lucisano Botelho Amaral

¹Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil

› Author Affiliations

Abstract

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ABSTRACT

Objective: The aim of this study was to evaluate in vitro the effect of photodynamic therapy (PDT) using LASER or light emitting diode (LED) on cariogenic bacteria (Streptococcus mutans [SM] and Lactobacillus casei [LC]) in bovine dentin. Materials and Methods: Twenty five fragments of dentin were contaminated with SM and LC strands and divided into five experimental groups according to the therapy they received (n = 5): C – control (no treatment), SCLED – no dye/LED application (94 J/cm²), SCLASER – no dye/LASER application (94 J/cm²), CCLED – dye/LED application (94 J/cm²) and CCLASER – dye/LASER application (94 J/cm²). The dye used was methylene blue at 10 mM. Dentin scrapes were harvested from each fragment and prepared for counts of colony forming units (CFU)/mL. The data were analyzed using Kruskal–Wallis, followed by Student–Newman–Keuls (⍺ =0.05). Results: Regarding SM, groups CCLASER and CCLED showed a significant reduction in CFU/mL, which was statistically superior to the SCLASER, SCLED and C groups. Regarding LC, the groups CCLASER and CCLED caused a significant reduction in CFU/mL when compared with SCLASER, which showed intermediate values. SCLED and C had a lesser effect on reducing CFU/mL, where the former showed values similar to those of SCLASER. Conclusions: In conclusion, PDT combined with LASER or LED and methylene blue had a significant antimicrobial effect on cariogenic bacteria in the dentin.

Key words:

Lactobacillus casei - photodynamic therapy - semi-conductor lasers - Streptococcus mutans

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