CC BY-NC-ND 4.0 · Eur J Dent 2016; 10(04): 469-474
DOI: 10.4103/1305-7456.195165
Original Article
Dental Investigation Society

Effect of platelet-rich plasma concentrations on the proliferation of periodontal cells: An in vitro study

Sara Tavassoli-Hojjati
1   Department of Pediatric Dentistry, Dental School, Islamic Azad University, Tehran Branch, Tehran, Iran
,
Mandana Sattari
2   Department of Immunology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
Tayebeh Ghasemi
3   Department of Oral and Maxillofacial Surgery, Tehran University of Medical Sciences, Tehran, Iran
,
Rahil Ahmadi
4   Department of Pediatric Dentistry, Dental School, Shahed University, Tehran, Iran
,
Abbas Mashayekhi
5   Department of Dermatology, Medical School, Islamic Azad University, Markazi, Iran
› Author Affiliations
Further Information

Publication History

Publication Date:
24 September 2019 (online)

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ABSTRACT

Objective: The purpose of this study was to evaluate the effect of different concentrations of platelet-rich plasma (PRP) on the proliferation of undifferentiated periodontal ligament (PDL) fibroblasts. Materials and Methods: The undifferentiated PDL fibroblasts were obtained from two healthy human premolar teeth and cultured in Dulbecco's modified Eagle's medium. Cell wells were divided into five groups. Experimental groups received 0.1%, 5%, or 50% PRP; the positive and negative control groups were cultured in fetal bovine serum (FBS) 12% and in a medium without FBS 12%, respectively. The plates were incubated at 37°C for 1, 2, 3, 4, and 7 days. PDL cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide proliferation assay. Statistical analysis of the data was accomplished using repeated measure ANOVA and Tukey's test. P < 0.05 was considered statistically significant. Results: The 5% PRP had the greatest effect on undifferentiated fibroblast proliferation, which was significant on the 3rd day. There was no significant difference between 0.1% PRP and positive control during the first 3 days. The group with 50% PRP presented significantly lower proliferation, compared to other experimental and control groups. Conclusions: It may be concluded that the growth-stimulating effect of PRP is dose dependent with the best results in low concentrations.