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CC BY-NC-ND 4.0 · Eur J Dent 2018; 12(01): 094-099
DOI: 10.4103/ejd.ejd_324_17
Original Article
Dental Investigation Society

Antimicrobial efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine, and ozonated water as irrigants in mesiobuccal root canals with severe curvature of mandibular molars

Sergio Luiz Pinheiro
1   Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil
,
Caio Cesar da Silva
1   Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil
,
Lucas Augusto da Silva
1   Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil
,
Marina P. Cicotti
1   Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil
,
Carlos Eduardo da Silveira Bueno
1   Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil
,
Carlos Eduardo Fontana
1   Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil
,
Letícia R. Pagrion
1   Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil
,
Natália P. Dalmora
1   Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil
,
Thaís T. Daque
1   Department of Endodontics, Pontifícia Universidade Católica de Campinas, Campinas, SP, Brazil
,
Francisco UF de Campos
2   Department of Endodontics, São Leopoldo Mandic, Campinas, SP, Brazil
› Author Affiliations
Further Information

Publication History

Publication Date:
13 September 2019 (online)

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ABSTRACT

Objective: The aim of this study is to evaluate the antimicrobial efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine, and ozonated water on biofilms of Enterococcus faecalis, Streptococcus mutans, and Candida albicans in mesiobuccal root canals with severe curvature of mandibular molars. Materials and Methods: This was an experimental ex vivo study in microbiologic laboratory. Sixty mesiobuccal root canals with severe curvature of mandibular molars were contaminated with standard strains of E. faecalis, S. mutans, and C. albicans. The specimens were randomly divided into four groups (n = 15) according to irrigating solution: SH: 2.5% sodium hypochlorite; CH: 2% chlorhexidine; O3: ozonated water; and control: double-distilled water. The mesiobuccal root canals of all groups were instrumented with the WaveOne Gold Primary reciprocating system. Three cycles of instrumentation with three short in-and-out brushing motions were performed: (1) in the coronal third, (2) in the middle third, and (3) in the apical third of the canal. A ProGlider file was used before the first cycle. Statistical Analysis: Statistical analysis was performed using one-way analysis of variance followed by Tukey’s multiple comparison test. Samples were collected for viable bacterial counts before and after instrumentation. Results: All groups showed significant biofilm reduction after irrigation (P < 0.01). After instrumentation, sodium hypochlorite (98.07%), chlorhexidine (98.31%), and ozonated water (98.02%) produced a significantly reduction in bacterial counts compared with double-distilled water (control, 72.98%) (P < 0.01). Conclusion: All irrigants tested in this study showed similar antimicrobial activity. Thus, ozonated water may be an option for microbial reduction in the root canal system.

Key words:

Endodontic treatment - irrigation - reciprocating system
 

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