Cytotoxicity evaluation of a new ozonized olive oil

ABSTRACT

Objective: The cytocompatibility of a new ozonized olive oil toward immortalized human gingival fibroblasts (HGFs) was evaluated and compared with two common antimicrobial agents based on chlorhexidine digluconate (CHX). Materials and Methods: The cytocompatibility of the samples was tested on immortalized HGF-1 cells by 3-(4, 5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay. The cells were incubated for 2 or 24 h with increasing dilution of ozonized olive oil or CHX agents. The percentage of viable cells was calculated relative to control cells set to 100%. Results: The ozonized olive oil is cytocompatible, and the viability values of the cells treated for 2 or 24 h with increasing concentrations of ozonized olive oil were significantly higher (P < 0.01) compared with the values obtained using CHX. Conclusions: The present data demonstrate that due to its cytocompatibility, the new ozonized olive oil could be considered an alternative antibacterial agent.

Key words:

3-(4, 5-dimethyl thiazolyl-2)-2 - 5-diphenyltetrazolium bromide test - cytotoxicity - gingival fibroblast - ozonized olive oil

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