2.11 Covalent Attachment on a Solid Support

R. M. Franzini 

Abstract

DNA oligonucleotides are polyfunctional molecules, and their various functional groups can interfere with DNA-encoded library synthesis. DNA that is attached to the support used for solid-phase synthesis has functional groups (e.g., exocyclic amines) protected. It is therefore possible to use harsher reaction conditions than would be possible with unprotected DNA, and the use of water-free solvents is also facilitated. For example, amide-bond formation on a solid support tends to be higher yielding than reactions in solution phase.

Schlüsselwörter

DNA-encoded libraries - solid-phase synthesis - on-DNA chemistry - amide-bond formation - protecting groups

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