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Scheuermann, J.  et al.: 2024 Science of Synthesis, 2023/5: DNA-Encoded Libraries DOI: 10.1055/sos-SD-241-00207
DNA-Encoded Libraries

2.10 Reversible Immobilization of DNA for Chemical Modification

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Book

Editors: Scheuermann, J. ; Li, Y. 

Authors: Barluenga, S. ; Bassi, G. ; Brunschweiger, A. ; Cai, B. ; Cazzamalli, S. ; Chheda, P. ; Cui, M. ; Cui, W. ; Fang, X. ; Farrera-Soler, L. ; Favalli, N. ; Feng, J.; Foley, T. L. ; Franzini, R. M. ; Georgiev, T. ; Gillingham, D. ; Gloger, A. ; Graham, J. D. ; Granados, A. ; Heiden, S.; Hou, W. ; Huang, Y. ; Keefe, A. D. ; Krusemark, C. J. ; Li, X. ; Li, Y. ; Lin, W. ; Litovchick, A.; Liu, G. ; Lu, X. ; Lucaroni, L. ; Ma, P. ; Migliorini, F. ; Molander, G. A. ; Neri, D. ; Nie, Q. ; Oehler, S. ; Prati, L. ; Puglioli, S. ; Reddavide, F. V. ; Satz, A. L. ; Sauter, B. ; Scheuermann, J. ; Schuman, D.; Simmons, N. ; Stanway-Gordon, H. A. ; Su, W. ; Sun, J. ; Thompson, M.; Vummidi, B. R.; Wang, X. ; Wang, Y. ; Wang, Z. ; Waring, M. J. ; Willems, S.; Winssinger, N. ; Xia, B. ; Xiong, F. ; Xu, H. ; Xu, L. ; Yang, G. ; Zhang, G. ; Zhang, Y. ; Zhou, Y. 

Title: DNA-Encoded Libraries

Print ISBN: 9783132455221; Online ISBN: 9783132437357; Book DOI: 10.1055/b000000342

1st edition © 2024. Thieme. All rights reserved.
Georg Thieme Verlag KG, Stuttgart

Subjects: Organic Chemistry

Science of Synthesis Reference Libraries



Parent publication

Title: Science of Synthesis

DOI: 10.1055/b-00000101

Series Editors: Fürstner, A. (Editor-in-Chief); Carreira, E. M.; Faul, M.; Kobayashi, S.; Koch, G.; Molander, G. A.; Nevado, C.; Trost, B. M.; You, S.-L.

Type: Multivolume Edition

Also available at
 


Abstract

Reversible immobilization of DNA molecules on anion-exchange resins allows for stringent washing after on-DNA modification reactions. Furthermore, such an approach allows reactions to be performed under water-free conditions that are otherwise incompatible with DNA-conjugation. Amide-bond formation and coupling of SnAP reagents are presented as examples of reactions on reversibly immobilized oligonucleotides for DNA-encoded library synthesis.

Key words

DNA-encoded libraries - anion-exchange resins - on-DNA chemistry - immobilization - amidation - SnAP reagents
 
  • 1 Lebl M. Curr. Opin. Drug Discov. Devel. 1999; 2: 385
  • 2 Halpin DR, Lee JA, Wrenn SJ, Harbury PB. PLoS Biol. 2004; 2: e175
  • 3 Franzini RM, Samain F, Abd Elrahman M, Mikutis G, Nauer A, Zimmermann M, Scheuermann J, Hall J, Neri D. Bioconjugate Chem. 2014; 25: 1453
  • 4 Flood DT, Asai S, Zhang X, Wang J, Yoon L, Adams ZC, Dillingham BC, Sanchez BB, Vantourout JC, Flanagan ME, Piotrowski DW, Richardson P, Green SA, Shenvi RA, Chen JS, Baran PS, Dawson PE. J. Am. Chem. Soc. 2019; 141: 9998
  • 5 Ruff Y, Martinez R, Pellé X, Nimsgern P, Fille P, Ratnikov M, Berst F. ACS Comb. Sci. 2020; 22: 120
  • 6 Flood DT, Knouse KW, Vantourout JC, Kitamura S, Sanchez BB, Sturgell EJ, Chen JS, Wolan DW, Baran PS, Dawson PE. ACS Cent. Sci. 2020; 6: 1789
  • 7 Flood DT, Zhang X, Fu X, Zhao Z, Asai S, Sanchez BB, Sturgell EJ, Vantourout JC, Richardson P, Flanagan ME, Piotrowski DW, Kölmel DK, Wan J, Tsai M.-H, Chen JS, Baran PS, Dawson PE. Angew. Chem. Int. Ed. 2020; 59: 7377
  • 8 Lin B, Lu W, Chen Z.-y, Zhang Y, Duan Y.-z, Lu X, Yan M, Zhang X.-j. Org. Lett. 2021; 23: 7381