Download PDF
Scheuermann, J.  et al.: 2024 Science of Synthesis, 2023/5: DNA-Encoded Libraries DOI: 10.1055/sos-SD-241-00219
DNA-Encoded Libraries

4.2 DEL Selection Using DNA-Programmed Affinity Labelling (DPAL)

More Information

Book

Editors: Scheuermann, J. ; Li, Y. 

Authors: Barluenga, S. ; Bassi, G. ; Brunschweiger, A. ; Cai, B. ; Cazzamalli, S. ; Chheda, P. ; Cui, M. ; Cui, W. ; Fang, X. ; Farrera-Soler, L. ; Favalli, N. ; Feng, J.; Foley, T. L. ; Franzini, R. M. ; Georgiev, T. ; Gillingham, D. ; Gloger, A. ; Graham, J. D. ; Granados, A. ; Heiden, S.; Hou, W. ; Huang, Y. ; Keefe, A. D. ; Krusemark, C. J. ; Li, X. ; Li, Y. ; Lin, W. ; Litovchick, A.; Liu, G. ; Lu, X. ; Lucaroni, L. ; Ma, P. ; Migliorini, F. ; Molander, G. A. ; Neri, D. ; Nie, Q. ; Oehler, S. ; Prati, L. ; Puglioli, S. ; Reddavide, F. V. ; Satz, A. L. ; Sauter, B. ; Scheuermann, J. ; Schuman, D.; Simmons, N. ; Stanway-Gordon, H. A. ; Su, W. ; Sun, J. ; Thompson, M.; Vummidi, B. R.; Wang, X. ; Wang, Y. ; Wang, Z. ; Waring, M. J. ; Willems, S.; Winssinger, N. ; Xia, B. ; Xiong, F. ; Xu, H. ; Xu, L. ; Yang, G. ; Zhang, G. ; Zhang, Y. ; Zhou, Y. 

Title: DNA-Encoded Libraries

Print ISBN: 9783132455221; Online ISBN: 9783132437357; Book DOI: 10.1055/b000000342

1st edition © 2024. Thieme. All rights reserved.
Georg Thieme Verlag KG, Stuttgart

Subjects: Organic Chemistry

Science of Synthesis Reference Libraries



Parent publication

Title: Science of Synthesis

DOI: 10.1055/b-00000101

Series Editors: Fürstner, A. (Editor-in-Chief); Carreira, E. M.; Faul, M.; Kobayashi, S.; Koch, G.; Molander, G. A.; Nevado, C.; Trost, B. M.; You, S.-L.

Type: Multivolume Edition

Also available at
 


Abstract

DNA-encoded libraries (DELs) have undergone significant development and are being widely adopted in drug discovery. As part of the evolution of DEL selections, the introduction of the DNA-programmed affinity labelling (DPAL) technique has extended the scope to include more-complex target systems beyond purified proteins immobilized on a solid matrix. In this review, detailed procedures for the application of DPAL-assisted DEL selections are compiled as a guide to the technique. Methods based on selections against in-solution proteins, DNA-encoded dynamic library (DEDL) selections, and selections against membrane proteins on a cell surface are included.

Key words

DNA-encoded libraries - DNA-programmed affinity labeling (DPAL) - high-throughput screening - DNA-encoded dynamic library (DEDL) - drug discovery - selection methodology
 
  • 1 Halford B. Chem. Eng. News 2017; 95: 28
  • 2 Mullard A. Nature (London) 2016; 530: 367
  • 3 Huang Y, Li X. ChemBioChem 2021; 22: 2384
  • 4 Huang Y, Li Y, Li X. Nat. Chem. 2022; 14: 129
  • 5 Song Y, Li X. Acc. Chem. Res. 2021; 54: 3491
  • 6 Li G, Liu Y, Liu Y, Chen L, Wu S, Liu Y, Li X. Angew. Chem. Int. Ed. 2013; 52: 9544
  • 7 Li G, Liu Y, Yu X, Li X. Bioconjugate Chem. 2014; 25: 1172
  • 8 Zhao P, Chen Z, Li Y, Sun D, Gao Y, Huang Y, Li X. Angew. Chem. Int. Ed. 2014; 53: 10056
  • 9 Shi B, Deng Y, Li X. ACS Comb. Sci. 2019; 21: 345
  • 10 Shi B, Deng Y, Zhao P, Li X. Bioconjugate Chem. 2017; 28: 2293
  • 11 Li G, Zheng W, Chen Z, Zhou Y, Liu Y, Yang J, Huang Y, Li X. Chem. Sci. 2015; 6: 7097
  • 12 Zhou Y, Li C, Peng J, Xie L, Meng L, Li Q, Zhang J, Li XD, Li X, Huang X, Li X. J. Am. Chem. Soc. 2018; 140: 15859
  • 13 Huang Y, Meng L, Nie Q, Zhou Y, Chen L, Yang S, Fung YME, Li X.-M, Huang C, Cao Y, Li Y, Li X. Nat. Chem. 2021; 13: 77