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Int J Angiol 2000; 9(3): 141-146
DOI: 10.1007/BF01616494
Original Articles

© Georg Thieme Verlag KG Stuttgart · New York

FR183998 protects against the increased microvascular permeability associated with ischemia-reperfusion injury in the canine lung

Y. Sunose1 , I. Takeyoshi1 , S. Ohwada1 , S. Iwazaki1 , H. Tsutsumi1 , Y. Kawashima1 , N. Oriuchi2 , K. Matsumoto3 , Y. Morishita1
  • 1Second Department of Surgery, Gunma University School of Medicine, Gunma, Japan
  • 2Department of Nuclear Medicine, Gunma University School of Medicine, Gunma, Japan
  • 3Department of Pathology, Nippon Medical School, Kawasaki, 211-8533, Japan
This paper was presented in part at the Professor Albert Senn: Young Investigator Award session, 41st Annual World Congress, International College of Angiology, Sapporo, Japan, July 1999.
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Publication Date:
24 April 2011 (online)

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Abstract

FR183998 (FR) is a Na+/H+ exchange inhibitor known to protect against cardiac ischemia-reperfusion (I/R) injury. The purpose of this study is to investigate the effects of FR on pulmonary I/R injury.

Eleven adult dogs were divided into two groups. FR (1mg/kg) was administered intravenously 5 min prior to ischemia and 5 min prior to reperfusion (FR group; n = 5), and a vehicle was injected in the same manner (Control group; n = 6). Warm ischemia was induced for 3 hr by clamping the left pulmonary artery and veins. The lung was then reperfused and animals were observed for 2 hr. Left pulmonary vascular resistance (L-PVR), cardiac output (CO), arterial oxygen pressure (PaO2), and alveolar arterial oxygen pressure difference (A-aDO2) were measured. The lung specimens were harvested for histological study, and polymorphonuclear neutrophils (PMNs) and the wet-to-dry lung weight ratio (W/D ratio) were quantified.99mTc-diethyltriaminepentaacetic acid (DTPA)-human serum albumin (HSA) scintigraphy was performed.

L-PVR, CO, PaO2, A-aDO2, and W/D ratio were maintained at significantly (p < 0.05) better levels in the FR group than in the control group. Histological findings revealed alveolar damage with interstitial edema in the control group and only slight interstitial edema in the FR group. PMN infiltration was significantly (p < 0.05) more severe in the control group than in the FR group. In the scintigrams,99mTc-DTPA-HSA was more accumulated in the left lung in the control group than in the FR group.

FR protects against the increased microvascular permeability resulting from I/R injury.