Electron-probe X-ray microanalysis of magnesium and sodium ion content in vascular smooth muscle cells from spontaneously hypertensive and normotensive rats

Abstract

Whereas in blood cells decreased magnesium concentrations and increased sodium concentrations in essential hypertension have often been described, only sparse data exist on cellular magnesium or sodium content in vascular smooth muscle cells. Therefore, in aortic smooth muscle cells from seven spontaneously hypertensive rats (SHR) of the Münster strain and seven normotensive Wistar-Kyoto rats (WKY), the intracellular magnesium and sodium content were measured by electron-probe X-ray microanalysis. Measurements were performed in aortic cryosections 3 μm thick. The magnesium ion content was 0.93 ± 0.17 g/kg dry weight in SHR vs 1.14 ± 0.12 g/kg dry weight in WKY (p < 0.05). Vascular smooth muscle sodium ion content was measured at 6.85 ± 0.59 g/kg dry weight in WKY and 12.47 ± 1.62 g/kg dry weight in SHR (p < 0.05). In conclusion, aortic smooth muscle cells from SHR are characterized by a markedly lowered intracellular magnesium ion content and increased sodium ion concentrations compared with normotensive cells. The results may be due to genetically determined disturbances in transmembrane magnesium and sodium ion transport. Cellular magnesium and sodium handling may be disturbed in SHR aortic smooth muscle as it is in hypertensive blood cells.