An Integrated Strategy to Qualitatively Differentiate Components of Raw and Processed Viticis Fructus Based on NIR, HPLC and UPLC-MS Analysis

Abstract

Viticis Fructus is a traditional Chinese herbal drug processed by various methods to achieve different clinical purposes. Thermal treatment potentially alters chemical composition, which may impact on effectiveness and toxicity. In order to interpret the constituent discrepancies of raw versus processed (stir-fried) Viticis Fructus, a multivariate detection method (NIR, HPLC, and UPLC-MS) based on metabonomics and chemometrics was developed. Firstly, synergy interval partial least squares and partial least squares-discriminant analysis were employed to screen the distinctive wavebands (4319 – 5459 cm⁻¹) based on preprocessed near-infrared spectra. Then, HPLC with principal component analysis was performed to characterize the distinction. Subsequently, a total of 49 compounds were identified by UPLC-MS, among which 42 compounds were eventually characterized as having a significant change during processing via the semiquantitative volcano plot analysis. Moreover, based on the partial least squares-discriminant analysis, 16 compounds were chosen as characteristic markers that could be in close correlation with the discriminatory near-infrared wavebands. Together, all of these characterization techniques effectively discriminated raw and processed products of Viticis Fructus. In general, our work provides an integrated way of classifying Viticis Fructus, and a strategy to explore discriminatory chemical markers for other traditional Chinese herbs, thus ensuring safety and efficacy for consumers.

Key words

Viticis Fructus - Vitex trifolia - Lamiaceae - liquid chromatography-tandem mass spectrometry - near-infrared - multivariate statistical analysis - quality control

• References

• 1 Okuyama E, Fujimori S, Yamazaki M, Deyama T. Pharmacologically active components of Viticis fructus (Vitex rotundifolia). II. The components having analgesic effects. Chem Pharm Bull 1998; 46: 655-662
• 2 National Pharmacopoeia Committee. Pharmacopoeia of the Peopleʼs Republic of China Part 1. Beijing: Chinese Medical Science and Technology Press; 2010
• 3 Ikawati Z, Wahyuono S, Maeyama K. Screening if several Indonesian medicinal plants for their inhibitory effect on histamine release from RBL-2H3 cells. J Ethnopharmacol 2001; 75: 249-256
• 4 Matsui M, Kumar-Roine S, Darius HT, Chinain M, Laurent D, Pauillac S. Characterisation of the anti-inflammatory potential of Vitex trifolia L. (Labiatae), a multipurpose plant of the Pacific traditional medicine. J Ethnopharmacol 2009; 126: 427-433
• 5 Matsui M, Adib-Conquy M, Coste A, Kumar-Roiné S, Pipy B, Laurent D, Pauillac S. Aqueous extract of Vitex trifolia L. (Labiatae) inhibits LPS-dependent regulation of inflammatory mediators in RAW 264.7 macrophages through inhibition of Nuclear Factor kappa B translocation and expression. J Ethnopharmacol 2012; 1431: 24-32
• 6 Singh V, Dayal R, Bartley JP. Chemical constituents of Vitex negundo leaves. Med Arom Plant Sci 2003; 25: 94-99
• 7 Li WX, Cui CB, Cai B, Yao XS. Labdane-type diterpenes as new cell cycle inhibitors and apoptosis inducers from Vitex trifolia L. J Asian Nat Prod Res 2005; 7: 95-105
• 8 Li WX, Cui CB, Cai B, Wang HY, Yao XS. Flavonoids from Vitex trifolia L. inhibit cell cycle progression at G2/M phase and induce apoptosis in mammalian cancer cells. J Asian Nat Prod Res 2005; 7: 615-626
• 9 Alam G, Wahyuono S, Ganjar IG, Hakim L, Timmerman H, Verpoorte R. Tracheospasmolytic activity of viteosin-A and vitexicarpin isolated from Vitex trifolia . Planta Med 2002; 68: 1047-1049
• 10 Zeng X, Fang Z, Wu Y, Zhang H. [Chemical constituents of the fruits of Vitex trifolia L]. Zhongguo Zhong Yao Za Zhi 1996; 21: 167-168 191
• 11 Hossain MM, Paul N, Sohrab MH, Rahman E, Rashid MA. Antibacterial activity of Vitex trifolia . Fitoterapia 2001; 72: 695-697
• 12 Kannathasan K, Senthikumar A, Venkatesalu V. In vitro antibacterial potential of some Vitex species against human pathogenic bacteria. Asian Pac J Trop Med 2011; 4: 645-648
• 13 Tiwari N, Luqman S, Masood N, Gupta MM. Validated high performance thin layer chromatographic method for simultaneous quantification of major iridoids in Vitex trifolia and their antioxidant studies. J Pharm Biomed Anal 2012; 61: 207-214
• 14 Armitage AK, Boswood J, Large BJ. Thioxanthines with potent brochodilator and coronary dilator properties. Br J Pharmacol Chemother 1961; 16: 59-76
• 15 Huang JY, Xu P, Zhu JG, Lou LY. Pharmacological study on anti-asthmatic effects of Vitex trifolia . J Jiangxi Univ Tradit Chin Med 2002; 4: 13-14
• 16 Guo CQ, Wang LC, Sun R. A summary of studies on Viticis Fructus. Jilin J Tradit Chin Med 1994; 5: 42
• 17 Wang D, Li QH, Zhou K. Research progress on chemistry, pharmacology and processing of Viticis Fructus. J Chin Med Pharm 2008; 36: 69-71
• 18 Tian ZK, Wang LZ, Yan GL, Yin YQ. HPLC fingerprint of Viticis Fructus. Chin Tradit Patent Med 2005; 27: 7-9
• 19 Xin HX, Hu Y, Zhang QY, Zheng HC, Qin LP. The content determination of flavonoids in Vitex trifolia L. from different districts. Pharm Care Res 2010; 10: 429-431
• 20 Guo CQ, Su DM, Cheng LF, Shen LX. GC-MS Analysis of volatile oil components in different processed products of simpleleaf shrub chastree (Vitex trifolia var. simplicifolia). Chin Tradit Herb Drug 1996; 27: 521-523
• 21 Xin HL, Hu Y, Zhang QY, Zheng HC, Qin LP. Cluster analysis on 4 medicinal Materia Medica from Vitex species by near-infrared diffuse reflectance spectrometry. Lishizhen Med Mater Med Res 2008; 19: 3037-3038
• 22 Wang LZ. Study on quality and fingerprint of Viticis Fructus. Heilongjiang J Tradit Chin Med 2003; 5: 12-14
• 23 Chen HY, Cheng WX, Feng Y, Gu K, Yang LJ, Zhang YP. Studies on flavonoids constituents of Vitex trifolia var. simplicifolia Cham. Nat Prod Res Dev 2008; 20: 582-584
• 24 Huang MY, Zhou LJ, Xie JM. A new taraxastane-type trierpene from Vitex trifolia var simplicifolia . Helv Chim Acta 2013; 96: 2040-2045
• 25 Ramachandran AG, Ramesh P, Subramanian SS. Two unusual flavones (artemisetin and 7-desmethyl aetemisetin) from the leaves of Vitex trifolia . Curr Sci 1975; 44: 214-216
• 26 Chen YS, Lin XY, Zhon LJ. Study on chemical constituents of Vitex trifolial. var. simplicifolia Cham. Nat Prod Res Dev 2011; 23: 1011-1013
• 27 Xin HL, Hu Y, Zhang QY. Study on chemical constituents of Vitext trifolia L. Acad J Second Military Med Univ 2006; 27: 1038-1040
• 28 Wu C, Zhang J, Yin ZQ. Study on chemical constituents of Vitext trifolia L. Pharm Bio Technol 2010; 17: 504-507
• 29 Tiwari N, Thakur J, Saikia D, Gupta MM. Antitubercular diterpenoids from Vitex trifolia . Phytomedicine 2013; 20: 605-610
• 30 Ramesh P, Nair AGR, Subramanian SS. Flavone glycosides of Vitex trifolia . Fitoterapia 1986; 57: 282-283