Quantification of Phenolic Compounds from Fadogia agrestis and Dietary Supplements using UHPLC-PDA-MS

Bharathi Avula; Ji-Yeong Bae; Vijayasankar Raman; Omer I. Fantoukh; Yan-Hong Wang; Ahmed G. Osman; Mei Wang; Zulfiqar Ali; Ikhlas A. Khan

doi:10.1055/a-0715-1801

Planta Medica, Table of Contents

Planta Med 2019; 85(02): 145-153
DOI: 10.1055/a-0715-1801

Natural Product Chemistry and Analytical Studies

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Quantification of Phenolic Compounds from Fadogia agrestis and Dietary Supplements using UHPLC-PDA-MS

Bharathi Avula^*

¹National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA

,

Ji-Yeong Bae^*

¹National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA

,

Vijayasankar Raman

¹National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA

,

Omer I. Fantoukh

¹National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA

²Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USA

,

Yan-Hong Wang

¹National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA

,

Ahmed G. Osman

¹National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA

,

Mei Wang

¹National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA

,

Zulfiqar Ali

¹National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA

,

Ikhlas A. Khan

¹National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA

²Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USA

› Author Affiliations

Abstract

Fadogia agrestis is used in traditional African medicine as an analgesic and for anti-inflammatory and aphrodisiac activities. An ultra-high-performance liquid chromatography method was developed for the determination of 11 chemical constituents from roots and aerial parts of F. agrestis. The separation was achieved within 7 min by using C-18 column material and a water/acetonitrile mobile phase, both containing 0.1% formic acid gradient system with a temperature of 45 °C. The method was validated for linearity, repeatability, limits of detection, and limits of quantification. The limits of detection of phenolic compounds were found to be in the range from 0.025 to 0.1 µg/mL. The wavelengths used for quantification with the photodiode array detector were 238, 254, 291, and 325 nm. Twelve of 17 dietary supplements contained phenolic compounds in the range from 0.3 to 2.7 mg/d. The phenolic compounds were not detected in five dietary supplements. Liquid chromatography-mass spectrometry coupled with electrospray ionization interface method is described for the identification and confirmation of compounds from plant samples and dietary supplements claiming to contain F. agrestis. This method involved the use of [M + H]⁺ and [M + Na]⁺ ions in the positive mode and [M − H]⁻ ions in the negative mode with extractive ion monitoring. The developed method is simple, economic, rapid, and especially suitable for quality control and chemical fingerprint analysis of F. agrestis.

Key words

Fadogia agrestis - Rubiaceae - phenolic compounds - dietary supplements - UHPLC-PDA-MS - quality control

Full Text

References

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