Habitual Light-intensity Physical Activity and ASC Methylation in a Middle-aged Population

Yuichiro Nishida; Megumi Hara; Yasuki Higaki; Naoto Taguchi; Kazuyo Nakamura; Hinako Nanri; Mikako Horita; Chisato Shimanoe; Jun Yasukata; Nobuyuki Miyoshi; Yosuke Yamada; Ken Higashimoto; Hidenobu Soejima; Keitaro Tanaka

doi:10.1055/a-0965-1374

International Journal of Sports Medicine, Table of Contents

Int J Sports Med 2019; 40(10): 670-677
DOI: 10.1055/a-0965-1374

Behavioural Sciences

Habitual Light-intensity Physical Activity and ASC Methylation in a Middle-aged Population

Yuichiro Nishida

¹Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan

,

Megumi Hara

¹Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan

,

Yasuki Higaki

²Laboratory of Exercise Physiology, Faculty of Sports and Health Science, Fukuoka University, Fukuoka, Japan

,

Naoto Taguchi

¹Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan

,

Kazuyo Nakamura

³Faculty of Nursing, Saint Maryʼs College, Kurume, Japan

,

Hinako Nanri

⁴Department of Nutrition and Metabolism, National Institute of Biomedical Innovation, Health and Nutrition, Tokyo, Japan

,

Mikako Horita

¹Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan

,

Chisato Shimanoe

⁵Clinical Research Center, Saga University Hospital, Saga, Japan

,

Jun Yasukata

²Laboratory of Exercise Physiology, Faculty of Sports and Health Science, Fukuoka University, Fukuoka, Japan

,

Nobuyuki Miyoshi

⁶Department of Childhood Care Education, Seika Women’s Junior College, Fukuoka, Japan

,

Yosuke Yamada

⁷Department of Nutritional Science, National Institute of Health and Nutrition, Tokyo, Japan

,

Ken Higashimoto

⁸Division of Molecular Genetics and Epigenetics, Department of Biomolecular Sciences, Faculty of Medicine, Saga University, Saga, Japan

,

Hidenobu Soejima

⁸Division of Molecular Genetics and Epigenetics, Department of Biomolecular Sciences, Faculty of Medicine, Saga University, Saga, Japan

,

Keitaro Tanaka

¹Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan

› Author Affiliations

Abstract

Apoptosis-associated, speck-like protein containing a caspase recruitment domain (ASC) plays an important role in inflammatory cytokine synthesis in peripheral blood mononuclear cells (PBMCs), and the expression of ASC is suppressed by increased methylation of its CpG sites. The current study investigated the longitudinal association of replacing sedentary time with light-intensity physical activity (LPA) or moderate to vigorous-intensity physical activity (MVPA) on the ASC methylation in middle-aged people. We investigated 1 238 individuals who participated in baseline and 5-year follow-up surveys of a population-based cohort study. Sedentary, LPA and MVPA time were objectively measured using accelerometers. ASC methylation in PBMCs was measured by pyrosequencing. Using a multiple linear regression and employing an isotemporal substitution model, the longitudinal associations of changes in the sedentary time, LPA and MVPA on the changes in the ASC methylation were analyzed after adjusting for potential confounders. Substituting 60 min per day of LPA for sedentary time was associated with 1.17 times (95% confidence interval 1.07, 1.27) higher ASC methylation levels (mean of 7 CpG sites, P<0.001). However, such effects were not seen for MVPA. These results suggest that substituting LPA for sedentary time may be linked with increased (favorable) ASC methylation as a potential biomarker of systemic inflammation.

Key words

DNA methylation - exercise - inflammation - isotemporal substitution

Full Text

References

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