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Exp Clin Endocrinol Diabetes 2020; 128(12): 819-826
DOI: 10.1055/a-1027-6620
Article

Short-Term Effects of Growth Hormone on Lipolysis, Glucose and Amino Acid Metabolism Assessed in Serum and Microdialysate of Healthy Young Men

Andreas Krebs
1   Department of Pediatrics and Adolescence Medicine, Faculty of Medicine, University of Freiburg, Germany
,
Andreas Baum   *
1   Department of Pediatrics and Adolescence Medicine, Faculty of Medicine, University of Freiburg, Germany
,
Jürgen Doerfer
1   Department of Pediatrics and Adolescence Medicine, Faculty of Medicine, University of Freiburg, Germany
,
Klaus Gempel   †
2   Department of Clinical Chemistry, München Klinik Schwabing, München, Germany
,
Michael Wurm   ‡
1   Department of Pediatrics and Adolescence Medicine, Faculty of Medicine, University of Freiburg, Germany
,
Corinna Brichta
1   Department of Pediatrics and Adolescence Medicine, Faculty of Medicine, University of Freiburg, Germany
,
Jörn Oliver Sass   §
1   Department of Pediatrics and Adolescence Medicine, Faculty of Medicine, University of Freiburg, Germany
,
Karl Winkler
3   Department of Clinical Chemistry, Faculty of Medicine, University of Freiburg, Germany
,
Karl Otfried Schwab
1   Department of Pediatrics and Adolescence Medicine, Faculty of Medicine, University of Freiburg, Germany
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Abstract

Objective We investigated direct effects of a therapeutic growth hormone dose on lipolysis, glucose and amino acid metabolism.

Methods This crossover microdialysis trial involved six healthy male volunteers receiving single subcutaneous injections of both growth hormone (0.035 mg/kg) and placebo (0.9% sodium chloride). The investigation comprised three test days with standard diet. The first day served for adaptation, the second and third one for determining study data during 9 night hours with or without growth hormone. Abdominal subcutaneous microdialysate and blood were continuously collected and forwarded to a separate room next door where hourly taken samples were centrifuged and frozen until analysed.

Results Growth hormone achieved the peak serum level after 3 h followed by a plateau-like course for the next 6 h. Glycerol in microdialysate started to rise 2 h following growth hormone injection achieving significance compared to placebo after 9 h (P<0.05). Serum glycerol increased 4 h after growth hormone administration achieving significance after 6 h (P<0.05). Glucose and amino acid concentrations showed neither in microdialysate nor in serum significant differences between growth hormone and placebo. Serum values of insulin and C-peptide revealed no significant difference between growth hormone and placebo.

Summary and Conclusion As the result of a high single subcutaneous dose of GH, persistent lipolysis can be shown in continuously collected microdialysate and blood, but no indication for gluconeogenesis or protein anabolism.

Key words

growth hormone - metabolic effects - pharmacokinetics - microdialysis - glycerol

* Current address: MediClin, MVZ Offenburg, Offenburg, Germany


† Current address: Laboratory Becker & Colleagues, Bamberg, Germany


‡ Current address: Hospital Barmherzige Brüder, Clinic St. Hedwig, Regensburg, Germany


§ Current address: Bonn-Rhein-Sieg University of Applied Sciences, Rheinbach, Germany




Publication History

Received: 24 July 2019
Received: 02 October 2019

Accepted: 14 October 2019

Article published online:
07 November 2019

© 2020. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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