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Planta Med 2020; 86(13/14): 967-975
DOI: 10.1055/a-1046-1022
Biological and Pharmacological Activity
Original Papers

Endophytic Microbiota Comparison of Dendrobium huoshanense Root and Stem in Different Growth Years

Shaotong Chen
1   Department of Biological and Pharmaceutical Engineering, West Anhui University, Luʼan, China
2   Department of Pharmacy, Anhui University of Chinese Medicine, Heʼfei, China
,
Jun Dai
1   Department of Biological and Pharmaceutical Engineering, West Anhui University, Luʼan, China
3   Anhui Province Traditional Chinese Medicine Resource Protection and Sustainable Utilization Engineering Laboratory, Luʼan, China
,
Xiangwen Song
1   Department of Biological and Pharmaceutical Engineering, West Anhui University, Luʼan, China
3   Anhui Province Traditional Chinese Medicine Resource Protection and Sustainable Utilization Engineering Laboratory, Luʼan, China
,
Xueping Jiang
1   Department of Biological and Pharmaceutical Engineering, West Anhui University, Luʼan, China
3   Anhui Province Traditional Chinese Medicine Resource Protection and Sustainable Utilization Engineering Laboratory, Luʼan, China
,
Qun Zhao
1   Department of Biological and Pharmaceutical Engineering, West Anhui University, Luʼan, China
3   Anhui Province Traditional Chinese Medicine Resource Protection and Sustainable Utilization Engineering Laboratory, Luʼan, China
,
Chuanbo Sun
1   Department of Biological and Pharmaceutical Engineering, West Anhui University, Luʼan, China
3   Anhui Province Traditional Chinese Medicine Resource Protection and Sustainable Utilization Engineering Laboratory, Luʼan, China
,
Cunwu Chen
1   Department of Biological and Pharmaceutical Engineering, West Anhui University, Luʼan, China
3   Anhui Province Traditional Chinese Medicine Resource Protection and Sustainable Utilization Engineering Laboratory, Luʼan, China
,
Naifu Chen
1   Department of Biological and Pharmaceutical Engineering, West Anhui University, Luʼan, China
3   Anhui Province Traditional Chinese Medicine Resource Protection and Sustainable Utilization Engineering Laboratory, Luʼan, China
,
Bangxing Han
1   Department of Biological and Pharmaceutical Engineering, West Anhui University, Luʼan, China
3   Anhui Province Traditional Chinese Medicine Resource Protection and Sustainable Utilization Engineering Laboratory, Luʼan, China
› Author AffiliationsSupported by: China Agriculture Research System CARS-21
Supported by:  The National Key Research and Development Program of China 2017YFC1700701
Supported by: Major Program of Increase or Decrease in the Central Government 2060302
Supported by: Anhui Provincial Science Fund for Distinguished Young Scholars 1808085J17
Supported by: Major Science Foundation of High Education of Anhui Province KJ2016SD61
Supported by: the Natural Science Foundation of China 81872955
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Abstract

The endophytic microbiome in medicinal plants is rich and diverse, but few studies have followed the endophytic microbiome of medicinal plants in different tissues with their growth. In this study, we examined the endophytic bacterial and fungal community structures associated with both the stem and root compartments of Dendrobium huoshanense at different growth years via high-throughput sequencing of 16S rRNA genes and nrDNA fragments of internal transcribed spacer regions. Results indicated that more diverse prokaryotic and fungal operational taxonomic units were detected in roots than in stems, and the alpha diversity of endophytic prokaryotic significantly differed among the 1-, 2-, and 3-year-old roots. The dominant bacterial phyla Proteobacteria Firmicutes, Actinobacteria, Bacteroidetes, and Acidobacteria, and fungal phyla Ascomycota, Basidiomycota, and Ascomycota were detected in the stems and roots with 3 growth years. Moreover, linear discriminant effect size analysis revealed 138 differentially abundant taxonomic clades in the bacterial level, and 197 in the fungal level in six groups. Our results provide evidence for endophytic microbiota communities depending on the tissues and growth years of D. huoshanense. The results from this study should be useful to better understand medicinal plant-microbe interactions.

Key words

Dendrobium huoshanense - endophytic microbiota - overlap - growth years

Supporting Information

    Details of sample information, the results COG functional prediction of the endophytic bacterial community and most discriminative bacterial and fungal community taxa (LEfSe analysis) of six groups are given in Supporting Information.

  • Supporting Information


Publication History

Received: 26 June 2019

Accepted after revision: 03 November 2019

Article published online:
25 November 2019

© 2020. Thieme. All rights reserved.

Georg Thieme Verlag KG
Stuttgart · New York

 

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