PDF herunterladen
Planta Med 2020; 86(03): 190-197
DOI: 10.1055/a-1068-7983
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Topical Application of 7,3′,4′-Trihydroxyisoflavone Alleviates Atopic Dermatitis-Like Symptoms in NC/Nga Mice

Sang Hun Park‡
1   Biomodulation, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea
,
Chang Hyung Lee‡
1   Biomodulation, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea
,
Ji Yun Lee
1   Biomodulation, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea
,
Hee Yang
2   Center for Food and Bioconvergence, Seoul National University, Seoul, Republic of Korea
3   Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea
,
Jong Hun Kim
4   Department of Food Science and Biotechnology, Sungshin University, Seoul, Republic of Korea
,
Jung Han Yoon Park
3   Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea
5   Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea
,
Jong-Eun Kim
6   Department of Food Science and Technology, Korea National University of Transportation, Jeungpyeong, Republic of Korea
,
Ki Won Lee
1   Biomodulation, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea
2   Center for Food and Bioconvergence, Seoul National University, Seoul, Republic of Korea
3   Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea
5   Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea
› InstitutsangabenGefördert durch: Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries 116030-3
Weitere Informationen

Publikationsverlauf

received 26. Juni 2019
revised 18. November 2019

accepted 21. November 2019

Publikationsdatum:
18. Dezember 2019 (online)

Auch verfügbar auf
   Lizenzen und Reprints

Abstract

Atopic dermatitis is a skin disease characterized by chronic inflammatory lesions, and new therapies are needed to address its rising prevalence. Soy isoflavone has been highlighted as a potential new cosmeceutical material that may have applications in atopic dermatitis care. We have developed a technique to attach an additional -OH group to the ortho position of -OH in the phenol ring using a special enzyme. By adding the -OH group to daidzein, 7,3′,4′-trihydroxyisoflavone can be generated for possible use as a cosmeceutical and functional food material. In this study, we sought to examine the anti-atopic effects of 7,3′,4′-trihydroxyisoflavone, an analog of daidzein. Topical application of 7,3′,4′-trihydroxyisoflavone reduced Dermatophagoides farina extract-induced atopic dermatitis symptoms in NC/Nga mice. Histological analysis demonstrated that 7,3′,4′-trihydroxyisoflavone suppressed D. farina extract-induced infiltration of eosinophils and mast cells into skin lesions. We also found that 7,3′,4′-trihydroxyisoflavone significantly reduces the D. farina extract-induced increases in serum IgE and macrophage-derived chemokine (CCL22) levels. We observed that 7,3′,4′-trihydroxyisoflavone suppresses atopic markers including macrophage-derived chemokine (CCL22) and thymus and activation-regulated chemokine (CCL17) in HaCaT cells. 7,3′,4′-Trihydroxyisoflavone also reduced TNF-α/IFN-γ-induced phosphorylation of ERK1/2 and JNK1/2. These results highlight several desirable properties of 7,3′,4′-trihydroxyisoflavone, which support its use as a cosmeceutical ingredient for the treatment of atopic dermatitis.

Key words

soybean - 7,3′,4′-trihydroxyisoflavone - atopic dermatitis - topical application
 

  • References

  • 1 Barbarot S, Auziere S, Gadkari A, Girolomoni G, Puig L, Simpson EL, Margolis DJ, de Bruin-Weller M, Eckert L. Epidemiology of atopic dermatitis in adults: Results from an international survey. Allergy 2018; 73: 1284-1293
    CrossrefPubMedGoogle Scholar
  • 2 Weidinger S, Novak N. Atopic dermatitis. Lancet 2016; 387: 1109-1122
    CrossrefPubMedGoogle Scholar
  • 3 Strathie Page S, Weston S, Loh R. Atopic dermatitis in children. Aust Fam Physician 2016; 45: 293-296
    PubMedGoogle Scholar
  • 4 Mayba JN, Gooderham MJ. Review of atopic dermatitis and topical therapies. J Cutan Med Surg 2017; 21: 227-236
    CrossrefPubMedGoogle Scholar
  • 5 Liu FT, Goodarzi H, Chen HY. IgE, mast cells, and eosinophils in atopic dermatitis. Clin Rev Allergy Immunol 2011; 41: 298-310
    CrossrefPubMedGoogle Scholar
  • 6 Kang H, Lee CH, Kim JR, Kwon JY, Son MJ, Kim JE, Lee KW. Theobroma cacao extract attenuates the development of Dermatophagoides farinae-induced atopic dermatitis-like symptoms in NC/Nga mice. Food Chem 2017; 216: 19-26
    CrossrefPubMedGoogle Scholar
  • 7 Kim JE, Kang YG, Park JS, Lim TG, Lee KW. Review of soybean phytochemicals and their bioactive properties relevant for skin health. J Food Nutr Res 2017; 5: 852-858
    PubMedGoogle Scholar
  • 8 Lokuruka M. Effects of processing on soybean nutrients and potential impact on consumer health: an overview. Afr J Food Agric Nutr Dev 2011; 11: 5000-5017
    PubMedGoogle Scholar
  • 9 Sarkar FH, Li Y. Soy isoflavones and cancer prevention: clinical science review. Cancer Investig 2003; 21: 744-757
    CrossrefPubMedGoogle Scholar
  • 10 Setchell KD. Phytoestrogens: the biochemistry, physiology, and implications for human health of soy isoflavones. Am J Clin Nutr 1998; 68: 1333S-1346S
    CrossrefPubMedGoogle Scholar
  • 11 Lee KW, Bode AM, Dong Z. Molecular targets of phytochemicals for cancer prevention. Nat Rev Cancer 2011; 11: 211-218
    CrossrefPubMedGoogle Scholar
  • 12 Kang NJ, Shin SH, Lee HJ, Lee KW. Polyphenols as small molecular inhibitors of signaling cascades in carcinogenesis. Pharmacol Ther 2011; 130: 310-324
    CrossrefPubMedGoogle Scholar
  • 13 Lee SH, Baek K, Lee JE, Kim BG. Using tyrosinase as a monophenol monooxygenase: A combined strategy for effective inhibition of melanin formation. Biotechnol Bioeng 2016; 113: 735-743
    CrossrefPubMedGoogle Scholar
  • 14 Tu HQ, Li XY, Tang MY, Gao JW, Xu LF, Chen ZQ, Gu H. Effects of tacrolimus on IFN-gamma signaling in keratinocytes: possible mechanisms by which tacrolimus affects IFN-gamma-dependent skin inflammation. Eur J Dermatol 2011; 21: 22-31
    PubMedGoogle Scholar
  • 15 Jung MR, Lee TH, Bang MH, Kim H, Son Y, Chung DK, Kim J. Suppression of thymus- and activation-regulated chemokine (TARC/CCL17) production by 3-O-beta-D-glucopyanosylspinasterol via blocking NF-kappaB and STAT1 signaling pathways in TNF-alpha and IFN-gamma-induced HaCaT keratinocytes. Biochem Biophys Res Commun 2012; 427: 236-241
    CrossrefPubMedGoogle Scholar
  • 16 Hongqin T, Xinyu L, Heng G, Lanfang X, Yongfang W, Shasha S. Triptolide inhibits IFN-gamma signaling via the Jak/STAT pathway in HaCaT keratinocytes. Phytother Res 2011; 25: 1678-1685
    CrossrefPubMedGoogle Scholar
  • 17 Kim JE, Son JE, Jang YJ, Lee DE, Kang NJ, Jung SK, Heo YS, Lee KW, Lee HJ. Luteolin, a novel natural inhibitor of tumor progression locus 2 serine/threonine kinase, inhibits tumor necrosis factor-alpha-induced cyclooxygenase-2 expression in JB6 mouse epidermis cells. J Pharmacol Exp Ther 2011; 338: 1013-1022
    CrossrefPubMedGoogle Scholar
  • 18 Lee CC, Dudonné S, Kim JH, Kim JS, Dubé P, Kim JE, Desjardins Y, Park JHY, Lee KW, Lee CY. A major daidzin metabolite 7,8,4′-trihydroxyisoflavone found in the plasma of soybean extract-fed rats attenuates monocyte-endothelial cell adhesion. Food Chem 2018; 240: 607-614
    CrossrefPubMedGoogle Scholar
  • 19 Choi KY, Jung E, Jung DH, An BR, Pandey BP, Yun H, Sung C, Park HY, Kim BG. Engineering of daidzein 3′-hydroxylase P450 enzyme into catalytically self-sufficient cytochrome P450. Microb Cell Fact 2012; 11: 81
    CrossrefPubMedGoogle Scholar